Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2

Kim, Tae Yun; Chung, Eun Joo; Sohn, Woon Mok; Hong, Sung Hong; Yong, Tai Soon

doi:10.1007/s00436-011-2546-6

Cited by 6 publications

(4 citation statements)

References 23 publications

(24 reference statements)

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“…TSPs in trematodes have an important role in the development, maturation and stability of the tegument and are involved in the immune evasion of schistosomes [27,28]. Recently, many studies have focused on some members of the TSPs family as a vaccine candidate against schistosomiasis [20,28], alveolar echinococcosis [29,30], filariasis [31] and clonorchiasis [32].…”

Section: Introductionmentioning

confidence: 99%

Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

et al. 2020

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Background: Cystic echinococcosis, caused by the cestode Echinococcus granulosus, is a neglected tropical disease with remarkable morbidity in humans and a problem of worldwide economic importance in livestock industry. Understanding the molecular basis of the parasite growth and development is essential for the disease diagnosis, management and control. The tetraspanin (TSP) family of proteins are transmembrane proteins with a role in many physiological processes of eukaryotic organisms. TSPs present in the tegumental surface of platyhelminths play pivotal roles in host-parasite interaction. However, little is known about the role of TSPs in growth and development in the Platyhelminthes. To understand the role of TSP1 in the growth and development of E. granulosus we investigated the effect of EgTSP1-specific long dsRNA in different in vitro stages of the parasite. Methods: Different stages of E. granulosus, protoscoleces and strobilated worms, were cultivated In vitro in di-phasic media. Using long dsRNA and two delivery methods, i.e. electroporation and electro-soaking, EgTSP1 silencing was performed with an EgTSP1-specific dsRNA. The TSP1 expression profile was assessed as well as the biological and ultrastructural properties of the parasites. Results: After three days of dsRNA treatment, EgTSP1 expression was significantly reduced in both stages of E. granulosus as compared to irrelevant/unrelated dsRNA and untreated controls. Silencing expression of EgTSP1 in different stages of E. granulosus resulted in reduced viability and body contractions, inhibition of protoscoleces evagination and distinctive tegumental changes. Ultrastructural morphology of the strobilated worms treated with EgTSP1-specific dsRNA was indicative of the microtriches impairments and vacuolated tegument compared to the control helminths. Conclusions: Results of the present study suggest that EgTSP1 plays important structural roles in tegument configuration in E. granulosus. EgTSP1 is proved to be a potential target for the development of vaccines and RNAi-based drugs.

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Section: Introductionmentioning

confidence: 99%

Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

et al. 2020

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“…In parasites, recent studies show that tetraspanins are associated with the development, maturation and stability of the tegument of trematodes, and are involved in the immune evasion of schistosome [ 12 – 16 ]. Of note, some members of the tetraspanin family have been targeted as candidate vaccines against schistosomiasis [ 17 – 20 ], clonorchiasis [ 21 ], alveolar echinococcosis [ 22 – 25 ], and filariasis [ 26 ]. For example, inoculation with the LELs of two tetraspanins of S. mansoni (Sm-TSP-1 and Sm-TSP-2) was confirmed to significantly decrease the adult worm burdens and liver egg burdens in experimentally infected mice [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus

Song

Xie

et al. 2015

Parasites Vectors

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BackgroundCystic echinococcosis (hydatid disease), caused by the tapeworm Echinococcus granulosus (class Cestoda; family Taeniidae), is a neglected tropical disease that results in morbidity and mortality in millions of humans, as well as in huge economic losses in the livestock industry globally. Proteins from the tetraspanin family in parasites have recently become regarded as crucial molecules in interaction with hosts in parasitism and are therefore suitable for the development of vaccines and diagnostic agents. However, no information is available to date on E. granulosus tetraspanin.MethodsIn this study, a uroplakin-I-like tetraspanin (Eg-TSP1) of E. granulosus was cloned and expressed in E. coli. The immunolocalization of Eg-TSP1 in different life stages of E. granulosus was determined using specific polyclonal antibody. The antibody and cytokine profiles of mice that immunized with recombinant Eg-TSP1 (rEg-TSP1) were measured for the immunogenicity analysis of this protein. Additionally, we use RNA interference method to explore the biological function of Eg-TSP1 in larva of E. granulosus.ResultsImmunofluorescence analysis showed that endogenous Eg-TSP1 mainly localized in the tegument of larvae and adults. Significantly elevated levels of antibodies IgG1 and IgG2a and of cytokines IFN-γ and IL-12 were observed in the sera of mice after immunization with rEg-TSP1, suggesting a typical T helper (Th)1-mediated immune response elicited by rEg-TSP1. On further probing the role of Eg-TSP1 in E. granulosus by RNA interference, we found that a thinner tegmental distal cytoplasm was induced in protoscoleces treated with siRNA-132 compared to controls.ConclusionsThis is the first report characterizing a tetraspanin from the tapeworm E. granulosus. Our results suggest that Eg-TSP1 is associated with biogenesis of the tegument and maintenance of structural integrity of E. granulosus and could therefore be a candidate intervention target for control of hydatid disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0926-y) contains supplementary material, which is available to authorized users.

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“…Two tetraspanins (TSP1 and TSP2) were identified in the outer surface of S. mansoni and S. japonicum and function in tegument renewal ( Tran et al, 2010 ), and these have been found to be promising vaccine candidates to protect against schistosomiasis ( Tran et al, 2010 ; Chen et al, 2016 ). Tetraspanin 2 was previously found in C. sinensis ( Kim et al, 2011 ), but the function of tetraspanins in C. sinensis remains unknown. Annexin is another important protein identified in the C. sinensis tegument.…”

Section: Discussionmentioning

confidence: 99%

Identification and Analysis of the Tegument Protein and Excretory-Secretory Products of the Carcinogenic Liver Fluke Clonorchis sinensis

Shi

Liang

et al. 2020

Front. Microbiol.

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Liver fluke proteins, including excretory-secretory products (ESPs) and tegument proteins, are critical for the pathogenesis, nutrient metabolism, etiology and immune response of liver cancer. To understand the functions of various proteins in Clonorchis sinensis physiology and human clonorchiasis, the ESPs and tegument proteins of C. sinensis were identified. Supernatants containing ESPs from adult C. sinensis after culture for 6 h were harvested and concentrated. The tegument was detached using a freeze/thaw method and successively extracted using various extraction buffers. The outer surface proteins of C. sinensis were labeled with biotin, and the biotinylated proteins were purified. The ESP, tegument and labeled outer surface proteins were identified and analyzed by high-resolution LC-MS/MS. The identified proteins were compared with those of other flukes, and the protein functions associated with pathogenesis, carcinogenesis and potential vaccine antigens and drug targets were predicted and analyzed. A total of 175 proteins were identified after the 6-h culture of C. sinensis ESPs. A total of 352 tegument proteins were identified through sequential solubilization of the isolated teguments, and a subset of these proteins were localized to the surface membrane of the tegument by labeling with biotin. Thirty identified proteins, including annexins, actin and tetraspanins, were identified as potential immunomodulators and promising vaccine antigens. Interestingly, among the 352 tegument proteins, as many as 155 were enzymes, and most were oxidoreductases, hydrolases or transferases. A comparison of the outer surface proteins of C. sinensis with those of other flukes indicated that flukes have some common outer surface proteins, such as actin, tetraspanin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and annexin. Granulin, thioredoxin peroxiredoxin, carbonyl reductase 1 and cystatin were identified in the C. sinensis proteome and predicted to be related to liver

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Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2

Cited by 6 publications

References 23 publications

Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus

Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus

Identification and Analysis of the Tegument Protein and Excretory-Secretory Products of the Carcinogenic Liver Fluke Clonorchis sinensis

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