Molecular Characterization of Chinese Grape Landraces (Vitis L.) Using Microsatellite DNA Markers

Li, Beibei; Jiang, Jianfu; Fan, Xianqun; Zhang, Ying; Sun, Han; Zhang, Guohai; Liu, Chonghuai

doi:10.21273/hortsci11802-17

Cited by 16 publications

(13 citation statements)

References 27 publications

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“…is one of the most economically important fruit crops cultivated and consumed worldwide (OIV, 2018). Vitis has a rich genetic diversity; there are more than 70 species within the genus, with most of the globally important cultivars assigned to Vitis vinifera (Li et al, 2017). Wild Vitis species as well as Muscadinia rotundifolia have been used for hybrid grape breeding as new cultivars and rootstocks, many of which provide resistance to pests and diseases (Carimi et al, 2011;Eibach et al, 2007;Smith et al, 2017).…”

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confidence: 99%

Cryopreservation of 12 Vitis Species Using Apical Shoot Tips Derived from Plants Grown In Vitro

Bettoni¹,

Kretzschmar²,

Bonnart³

et al. 2019

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The availability of and easy access to diverse Vitis species are prerequisites for advances in breeding programs. Plant genebanks usually maintain collections of Vitis taxa as field collections that are vulnerable to biotic and abiotic stresses. Cryopreservation has been considered an ideal method of preserving these collections as safety back-ups in a cost-effective manner. We report a droplet vitrification method used to cryopreserve 12 Vitis species (Vitis vinifera cvs. Chardonnay and ‘Riesling, V. actinifolia, V. aestivalis, V. jacquemontii, V. flexuosa, V. palmata, V. riparia, V. rupestris, V. sylvestris, V. ficifolia, V. treleasi, and V. ×novae angeliae) using shoot tips excised from plants grown in vitro. Our results demonstrated wide applicability of this technique, with regrowth levels at least 43% for 13 genotypes representing 12 Vitis species. We demonstrated that the droplet vitrification procedure can be successfully replicated by technical staff, thus suggesting that this method is ready for implementation.

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confidence: 99%

Cryopreservation of 12 Vitis Species Using Apical Shoot Tips Derived from Plants Grown In Vitro

Bettoni¹,

Kretzschmar²,

Bonnart³

et al. 2019

horts

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“…Internationally, France, Germany, Italy, and other countries have used the nine SSR markers to establish a molecular database of grape cultivars (Maul and T€ opfer, 2015), providing an inquiry service for grape research workers. Furthermore, Li et al (2017Li et al ( , 2018 showed that the nine SSR markers can be used to identify the Chinese grape landraces and Euro-American hybrids. = the inbreeding coefficient within subpopulations relative to the total; F is = the inbreeding coefficient within individuals relative to the subpopulation; F it = the inbreeding coefficient within individuals relative to the total.…”

Section: Resultsmentioning

confidence: 99%

“…Klein et al (2018) also used high-throughput sequencing to clarify evolutionary relationships among North American Vitis species. Because SSR markers show high levels of polymorphisms, reproducibility, and codominant inheritance (Aradhya et al, 2013;Li et al, 2017), they have been widely used to study the genetic diversity of grapes (Cao et al, 2020;Guo et al, 2010), sugarcane [Saccharum officinarum (Ali et al, 2019)], wheat [Triticum aestivum (Abbasov et al, 2018)], cabbage [Brassica oleracea (El-Esawi et al, 2016)], and hazelnuts [Corylus heterophylla (Zhao et al, 2020)].…”

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confidence: 99%

Genetic Diversity and Population Structure Analysis of Chinese Wild Grape Using Simple Sequence Repeat Markers

Li¹,

Fan²,

Zhang³

et al. 2021

J. Amer. Soc. Hort. Sci.

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Chinese wild Vitis is a useful gene source for resistance to biotic and abiotic stresses, although there is little research on its genetic diversity and structure. In this study, nine simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure among 100 Vitis materials. These materials included 77 indigenous accessions representing 23 of 38 wild Vitis species/cultivars in China, 18 V. vinifera cultivars, and the five North American species V. aestivalis, V. girdiana, V. monticola, V. acerifolia, and V. riparia. The SSR loci used in this study for establishing an international database (Vitis International Variety Catalogue) revealed a total of 186 alleles in 100 Vitis accessions. The mean values for the gene diversity (GD) and polymorphism information content (PIC) per locus were 0.91 and 0.90, respectively, which indicates that the discriminatory power of the markers is high. Based on the genetic distance data, the 100 Vitis accessions were divided into five primary clusters by cluster analysis, and five populations by structure analysis; these results indicate these Chinese wild grapes were more genetically close to European grapes than to North American species. In addition, the clustering patterns of most accessions correlated with the geographic distribution. An analysis of molecular variance (AMOVA) revealed that 3.28%, 3.27%, and 93.46% of the variance occurred between populations, between individuals within populations, and between individuals within the entire population, respectively. In addition, we identified three previously undescribed accessions (Wuzhi-1, MZL-5, and MZL-6) by cluster analysis. Our results reveal a high level of genetic diversity and variability in Vitis from China, which will be helpful in the use of genetic resources in future breeding programs. In addition, our study demonstrates that SSR markers are highly suitable for further genetic diversity analyses of Chinese wild grapes.

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“…In recent years, genomic studies on grapevine cultivars have provided a significant quantity of genomic information, including evolution and domestication processes (Zhou et al, 2019), molecular markers and transferability (Li et al, 2017;Zou et al, 2020), sex determination (Massonnet et al, 2020), and population genetics (Zhou et al, 2019). Furthermore, V. vinifera cultivar genomic information has been utilized as evidence to support ancestral hexaploidization in major angiosperm phyla.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar

et al. 2021

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The Munake grape cultivar produces uniquely flavored and high-quality fruits. Despite the numerous beneficial agronomic traits of Munake, there are few genetic resources available for this cultivar. To address this knowledge gap, the entire genome was sequenced using whole-genome sequencing approaches and compared with a Vitis vinifera L. reference genome. This study describes more than 3 million single nucleotide polymorphism (SNP), 300,000 insertion and deletion (InDel), 14,000 structural variation (SV), and 80,000 simple sequence repeat (SSR) markers (one SSR per 4.23 kb), as well as their primers. Among the SSRs, 44 SSR primer pairs were randomly selected and validated by polymerase chain reaction (PCR), allowing discrimination between the different Munake cultivar genotypes. The genetic data provided allow a deeper understanding of Munake cultivar genomic sequence and contribute to better knowledge of the genetic basis behind its key agronomic traits.

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Molecular Characterization of Chinese Grape Landraces (Vitis L.) Using Microsatellite DNA Markers

Cited by 16 publications

References 27 publications

Cryopreservation of 12 Vitis Species Using Apical Shoot Tips Derived from Plants Grown In Vitro

Cryopreservation of 12 Vitis Species Using Apical Shoot Tips Derived from Plants Grown In Vitro

Genetic Diversity and Population Structure Analysis of Chinese Wild Grape Using Simple Sequence Repeat Markers

Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar

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