Molecular characterization of chikungunya virus isolates from two localized outbreaks during 2014-2019 in Kerala, India

Balakrishnan, Anukumar; Devi, Thounaojam Asia; Koshy, Jijo; Nikhil, N T; Sugunan, A P

doi:10.1007/s00705-021-05186-9

Cited by 3 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, both studies analyzed anti-CHIKV IgG seroprevalence in populations that had been facing an outbreak. This silent CHIKV circulation has already been described in areas that have experienced epidemics after this scenario, in which asymptomatic cases were detected through the presence of anti-CHIKV IgG in a substantially higher number than symptomatic acute cases [18,[53][54][55][56]. These data can represent a warning to the health authorities of SJdRP and other places in Brazil and in the world where CHIKV has been introduced.…”

Section: Discussionsupporting

confidence: 55%

Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015–2019

Zini,

Ávila,

Cezarotti

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Background Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand the lack of CHIKV epidemics in São José do Rio Preto (SJdRP), São Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion in a prospective study of asymptomatic individuals and detecting anti-CHIKV IgM in individuals suspected of dengue infection, as well as CHIKV presence in Aedes mosquitoes. The opportunity to assess two different groups (symptomatic and asymptomatic) exposed at the same geographic region aimed to broaden the possibility of identifying the viral circulation, which had been previously considered absent. Methodology/principal findings Based on a prospective population study model and demographic characteristics (sex and age), we analyzed the anti-CHIKV IgG seroconversion rate in 341 subjects by ELISA over four years. The seroprevalence increased from 0.35% in the first year to 2.3% after 3 years of follow-up. Additionally, we investigated 497 samples from a blood panel collected from dengue-suspected individuals during the 2019 dengue outbreak in SJdRP. In total, 4.4% were positive for anti-CHIKV IgM, and 8.6% were positive for IgG. To exclude alphavirus cross-reactivity, we evaluated the presence of anti-Mayaro virus (MAYV) IgG by ELISA, and the positivity rate was 0.3% in the population study and 0.8% in the blood panel samples. In CHIKV and MAYV plaque reduction neutralization tests (PRNTs), the positivity rate for CHIKV-neutralizing antibodies in these ELISA-positive samples was 46.7%, while no MAYV-neutralizing antibodies were detected. Genomic sequencing and phylogenetic analysis revealed CHIKV genotype ECSA in São José do Rio Preto, SP. Finally, mosquitoes collected to complement human surveillance revealed CHIKV positivity of 2.76% of A. aegypti and 9.09% of A. albopictus (although it was far less abundant than A. aegypti) by RT–qPCR. Conclusions/significance Our data suggest cryptic CHIKV circulation in SJdRP detected by continual active surveillance. These low levels, but increasing, of viral circulation highlight the possibility of CHIKV outbreaks, as there is a large naïve population. Improved knowledge of the epidemiological situation might aid in outbreaks prevention.

show abstract

Section: Discussionsupporting

confidence: 55%

Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015–2019

Zini,

Ávila,

Cezarotti

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

“…Then, the wide expansion of IOL sub-lineage E1-K211E/E2-V264A occurred after the virus acquired the mutation of E1-I317V associated with the later outbreaks. The IOL with these three mutations clustered into the distinct clade that shared a tMRCA in 2012, while the earliest detection of this clade virus was around October 2014 in Kerala, the southern state of India [39]. However, the individual branches, including Singaporean strains [21] and Indian strains [40] observed between E1-K211E/E2-V264A and E1-K211E/E2-V264A/E1-I317V clusters indicated inadequate sequence sampling, especially during the inter-epidemic (or inter-outbreak) period during which IOL variants were possibly diverse.…”

Section: Discussionmentioning

confidence: 99%

Spread of a Novel Indian Ocean Lineage Carrying E1-K211E/E2-V264A of Chikungunya Virus East/Central/South African Genotype across the Indian Subcontinent, Southeast Asia, and Eastern Africa

et al. 2022

View full text Add to dashboard Cite

The Indian Ocean Lineage (IOL) of the chikungunya virus (CHIKV) East/Central/South African (ECSA) genotype, which originated in Kenya, spread to the Indian ocean and the Indian subcontinent, and then expanded through Southeast Asia in the previous decade. It carried an adaptive mutation E1-A226V, which enhances CHIKV replication in Aedes albopictus. However, the IOL CHIKV of the most recent outbreaks during 2016–2020 in India, Pakistan, Bangladesh, the Maldives, Myanmar, Thailand, and Kenya lacked E1-A226V but carried E1-K211E and E2-V264A. Recent CHIKV genome sequences of the Maldives and Thailand were determined, and their phylogenetic relationships were further investigated together with IOL sequences reported in 2004–2020 in the database. The results showed that the ancestral IOLs diverged to a sub-lineage E1-K211E/E2-V264A, probably in India around 2008, and caused sporadic outbreaks in India during 2010–2015 and in Kenya in 2016. The massive expansion of this new sub-lineage occurred after the acquisition of E1-I317V in other neighboring and remote regions in 2014–2020. Additionally, the phylogenetic tree indicated that independent clades formed according to the geographical regions and introduction timing. The present results using all available partial or full sequences of the recent CHIKVs emphasized the dynamics of the IOL sub-lineages in the Indian subcontinent, Southeast Asia, and Eastern Africa.

show abstract

Unveiling Actin Cytoskeleton Role in Mediating Chikungunya-Associated Arthritis: An Integrative Proteome–Metabolome Study

Mahin,

Chikmagalur Ravindra,

Ramesh

et al. 2024

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Molecular characterization of chikungunya virus isolates from two localized outbreaks during 2014-2019 in Kerala, India

Cited by 3 publications

References 16 publications

Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015–2019

Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015–2019

Spread of a Novel Indian Ocean Lineage Carrying E1-K211E/E2-V264A of Chikungunya Virus East/Central/South African Genotype across the Indian Subcontinent, Southeast Asia, and Eastern Africa

Unveiling Actin Cytoskeleton Role in Mediating Chikungunya-Associated Arthritis: An Integrative Proteome–Metabolome Study

Contact Info

Product

Resources

About