Molecular characterization of cell death induced by a compatible interaction between Fusarium oxysporum f. sp. linii and flax (Linum usitatissimum) cells

Hano, Christophe; Addi, Mohamed; Fliniaux, Ophélie; Bensaddek, Lamine; Duverger, Eric; Mesnard, François; Lamblin, Frédéric; Lainé, Éric

doi:10.1016/j.plaphy.2008.02.004

Cited by 59 publications

(46 citation statements)

References 51 publications

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“…The presence of these putative cis-acting elements appeared to be consistent with the observed enhanced LuPLR1 gene expression in response to a fungal elicitor in flax cell suspension (Hano et al 2006b(Hano et al , 2008Attoumbre et al 2006). Moreover, Torres et al (2005) have also demonstrated an insecticidal activity of SECO, the PLR product.…”

Section: Luplr1 In Silico and Functional Analysessupporting

confidence: 73%

Abscisic acid regulates pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol accumulation in developing flax (Linum usitatissimum L.) seeds

Renouard

Corbin

López

et al. 2011

Planta

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Secoisolariciresinol diglucoside (SDG), the main phytoestrogenic lignan of Linum usitatissimum, is accumulated in the seed coat of flax during its development and pinoresinol-lariciresinol reductase (PLR) is a key enzyme in flax for its synthesis. The promoter of LuPLR1, a flax gene encoding a pinoresinol lariciresinol reductase, contains putative regulatory boxes related to transcription activation by abscisic acid (ABA). Gel mobility shift experiments evidenced an interaction of nuclear proteins extracted from immature flax seed coat with a putative cis-acting element involved in ABA response. As ABA regulates a number of physiological events during seed development and maturation we have investigated its involvement in the regulation of this lignan synthesis by different means. ABA and SDG accumulation time courses in the seed as well as LuPLR1 expression were first determined in natural conditions. These results showed that ABA timing and localization of accumulation in the flax seed coat could be correlated with the LuPLR1 gene expression and SDG biosynthesis. Experimental modulations of ABA levels were performed by exogenous application of ABA or fluridone, an inhibitor of ABA synthesis. When submitted to exogenous ABA, immature seeds synthesized 3-times more SDG, whereas synthesis of SDG was reduced in immature seeds treated with fluridone. Similarly, the expression of LuPLR1 gene in the seed coat was up-regulated by exogenous ABA and down-regulated when fluridone was applied. These results demonstrate that SDG biosynthesis in the flax seed coat is positively controlled by ABA through the transcriptional regulation of LuPLR1 gene.

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Section: Luplr1 In Silico and Functional Analysessupporting

confidence: 73%

Abscisic acid regulates pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol accumulation in developing flax (Linum usitatissimum L.) seeds

Renouard

Corbin

López

et al. 2011

Planta

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“…BY-2 cells that were collected 48 h after treatment were examined under bright field and fluorescence microscopy [26].…”

Section: Cell Wall Autofluorescencementioning

confidence: 99%

“…For the coloration of lignin, BY-2 cells were collected and treated with 2.5 % (w/v) phloroglucinol in 6 M HCl for 30 min [26]. A total of 0.5 g of dried cells was used for the lignin content measurement as described by Liu et al [28].…”

Section: Lignin Determinationmentioning

confidence: 99%

Dry mycelium of Penicillium chrysogenum activates defense responses and restricts the spread of Tobacco Mosaic Virus in tobacco

Zhong

Peng

Chen

et al. 2015

Physiological and Molecular Plant Pathology

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“…Medium (or apoplast) alkalization induced respiratory burst oxidase homolog (RBOH) activation, probably as a result of induced depolarization of the plasma membrane and subsequent K + /H + exchange, followed by Ca 2 + influx/ Cl 2 efflux (109, 137, 163). Transient extracellular alkalinization is an essential factor in induction of the signal transduction pathway during induction of the defense response and plant programmed cell death (PCD) (34, 63,132,134). In this connection, changes in external pH rapidly alter plant gene expression and modulate host responses, similarly to elicitors (79).…”

Section: Host Response To Ambient Phmentioning

confidence: 99%

Virulence Regulation of Phytopathogenic Fungi by pH

Alkan

Espeso

Prusky³

2013

Antioxidants & Redox Signaling

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Significance: Postharvest pathogens can start its attack process immediately after spores land on wounded tissue, whereas other pathogens can forcibly breach the unripe fruit cuticle and then remain quiescent for months until fruit ripens and then cause major losses. Recent Advances: Postharvest fungal pathogens activate their development by secreting organic acids or ammonia that acidify or alkalinize the host ambient surroundings. Critical Issues: These fungal pH modulations of host environment regulate an arsenal of enzymes to increase fungal pathogenicity. This arsenal includes genes and processes that compromise host defenses, contribute to intracellular signaling, produce cell wall-degrading enzymes, regulate specific transporters, induce redox protectant systems, and generate factors needed by the pathogen to effectively cope with the hostile environment found within the host. Further, evidence is accumulating that the secreted molecules (organic acids and ammonia) are multifunctional and together with effect of the ambient pH, they activate virulence factors and simultaneously hijack the plant defense response and induce program cell death to further enhance their necrotrophic attack. Future Directions: Global studies of the effect of secreted molecules on fruit pathogen interaction, will determine the importance of these molecules on quiescence release and the initiation of fungal colonization leading to fruit and vegetable losses.

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Molecular characterization of cell death induced by a compatible interaction between Fusarium oxysporum f. sp. linii and flax (Linum usitatissimum) cells

Cited by 59 publications

References 51 publications

Abscisic acid regulates pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol accumulation in developing flax (Linum usitatissimum L.) seeds

Abscisic acid regulates pinoresinol–lariciresinol reductase gene expression and secoisolariciresinol accumulation in developing flax (Linum usitatissimum L.) seeds

Dry mycelium of Penicillium chrysogenum activates defense responses and restricts the spread of Tobacco Mosaic Virus in tobacco

Virulence Regulation of Phytopathogenic Fungi by pH

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