Molecular characterization of capsid protein gene of potato virus X from Pakistan

A Brief Review for Identification and Detection of Potato Viruses

“…Primers having 20 nucleotides require increase of 2°C for every addition of A or T and 4°C for G or C. RT-PCR was applied in detecting PVY, PLRV, PVX, PVA, PVS and PVM (Nie et al, 2008;Nosheen et al, 2013). RT-PCR has been applied for cloning, molecular detection and sequence analysis of CP gene of PVY and PVX (Jamal et al, 2012). Very expensive costly molecular biology grade consumable and costly equipment (thermocycler) are necessary of PCR and these techniques are prone to render false positives due to its extremely sensitivity coupled with the ease of contamination by contaminated reagents, gloves, hair, skin, aerosols, commercial preparations of TaqDNA polymerase, or even autoclaved material containing target sequences (Dwyer and Saksena, 1992).…”

“…Complementary DNA (cDNA) libraries of coat protein gene have been prepared by using the genomic RNAs of PLRV, PVX and PVY by coat protein (CP) gene specific sense and antisense primes. PVX has cloned and sequenced CP gene (613 bp) form Pakistani isolate (HE577130) and nucleotide evidence showed its maximum genetic similarity with USSR isolates of PVX (Jamal et al, 2012) while this was first report of PVX form Pakistan. Abbas et al, (2014) has reported recombinant strain of PVY on the basic of biological, serological and nucleotide evidence of CP gene from two Pakistani isolates (JQ425622 and JQ518266).…”

Different Techniques for Diagnostic of Potato Viruses: A Brief Review

“…Complementary DNA (cDNA) libraries of coat protein gene have been prepared by using the genomic RNAs of PLRV, PVX and PVY by coat protein (CP) gene specific sense and antisense primers. Jamal et al (2012) has cloned and sequenced CP gene (613 bp) of PVX from Batool, S., Aurangzeb, W., Zainab, R., & Menghwar, S. (2016). Different techniques for diagnostic of potato viruses : a brief review .…”