Molecular characterization of calicivirus strains detected in outbreaks of gastroenteritis in Argentina

Gomes, Karina A.; Stupka, Juan Andrés; Gómez, Jorge; Parra, Gabriel I.

doi:10.1002/jmv.20989

Cited by 35 publications

(27 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The GII.16 and GII.17 strains were found circulating simultaneously during a Rotavirus-A G9 gastroenteritis outbreak that occurred in 2005 in AC (North Region) , Tort et al 2010. These genotypes were also found circulating in South Korea, 2007, Argentina, 2005, and Thailand, 2006 (Gomes et al 2008, Chung et al 2010, Kittigul et al 2010. In Brazil, GII.12 was observed circulating in three different regions: Northeast, Southeast and South.…”

Section: Discussionmentioning

confidence: 95%

Genetic diversity of noroviruses in Brazil

Fioretti

Ferreira

Victoria

et al. 2011

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 95%

Genetic diversity of noroviruses in Brazil

Fioretti

Ferreira

Victoria

et al. 2011

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Norovirus GII.17 is of clinical relevance as it has been implicated in nosocomial NoV infection (Sukhrie et al 2011) and chronic NoV infection in a kidney transplant patient (Schorn et al 2010). The genotype has also been widely reported in children with gastroenteritis in Central and South America (Bucardo et al 2009;Ferreira et al 2012;Gomes et al 2008), Korea (Park et al 2011) and Thailand (Kittigul et al 2010). …”

Section: Discussionmentioning

confidence: 99%

Norovirus GII.17 Predominates in Selected Surface Water Sources in Kenya

et al. 2014

View full text Add to dashboard Cite

In this study the prevalence and genotypes of NoVs in selected water sources from rural, urban and refugee settings in Kenya was investigated. Ten liters each of river, household and borehole water were collected in rural (Mboone river), urban (Nairobi and Mutoine river), and refugee (Dadaab refugee camp) settings. Noroviruses were recovered from the water samples by a glass wool adsorption-elution technique and/or PEG/NaCl precipitation.Nucleic acid was extracted using the automated MagNA Pure platform. Noroviruses were detected with singleplex real-time reverse transcription-polymerase chain reaction assays and characterised by nucleotide sequence analysis. Noroviruses were detected in 63% (25/40) of the selected water samples comprising GII (42.5%), GI (2.5%) and mixed GI/GII (17.5%) positive samples. The prevalence of NoVs in the Mutoine river (urban area) was higher than in the Mboone river (rural area) (P=0.0013). Norovirus GI.1, GI.3, GI.9, GII.4, GII.6, GII.12, GII.16 and GII.17 were identified, with GII.17 accounting for 76% (16/21) of the typed strains. The NoV GII.17 predominance differs to other studies in Africa and further surveillance of NoVs in clinical and environmental settings is required to clarify/elucidate this observation. As information regarding NoVs in Kenyan water sources is limited this report provides valuable new data on NoV genotypes circulating in environmental water sources and the surrounding communities in Kenya.

show abstract

“…Evidence based on recombinants generated from a small, ssDNA virus, maize streak virus, demonstrated that fragments of genetic material only function optimally if they reside within genomes similar to those in which they evolved (Martin et al, 2005 , 2006). Later, GIIb recombinants were identified in a wide range of countries, indicating their widespread distribution across continents (Bruggink & Marshall, 2009;Fukuda et al, 2008;Gomes et al, 2007). It is possible that recombination was able to give rise to novel NoV strains capable of epidemic spread in human In vitro MNV recombination populations.…”

Section: Discussionmentioning

confidence: 99%

Experimental evidence of recombination in murine noroviruses

Mathijs

Muylkens

Mauroy

et al. 2010

Journal of General Virology

View full text Add to dashboard Cite

Based on sequencing data, norovirus (NoV) recombinants have been described, but no experimental evidence of recombination in NoVs has been documented. Using the murine norovirus (MNV) model, we investigated the occurrence of genetic recombination between two co-infecting wild-type MNV isolates in RAW cells. The design of a PCR-based genotyping tool allowed accurate discrimination between the parental genomes and the detection of a viable recombinant MNV (Rec MNV) in the progeny viruses. Genetic analysis of Rec MNV identified a homologous-recombination event located at the ORF1-ORF2 overlap. Rec MNV exhibited distinct growth curves and produced smaller plaques than the wild-type MNV in RAW cells. Here, we demonstrate experimentally that MNV undergoes homologous recombination at the previously described recombination hot spot for NoVs, suggesting that the MNV model might be suitable for in vitro studies of NoV recombination. Moreover, the results show that exchange of genetic material between NoVs can generate viruses with distinct biological properties from the parental viruses. INTRODUCTIONNoroviruses (NoVs) are an important cause of acute gastroenteritis in humans worldwide. Since the first description of NoVs in humans in 1968, NoV infections have also been detected in domestic and captive wild animals (Scipioni et al., 2008). The genus Norovirus belongs to the family Caliciviridae. NoVs are non-enveloped viruses with a single-stranded, positive-sense, polyadenylated RNA genome composed of around 7500 nt. Three overlapping ORFs encode the non-structural (ORF1) and structural (ORF2 and ORF3) viral proteins. The ORF1-encoded polyprotein is cleaved further by the viral proteinase into six mature products with the gene order N-term, NTPase, p18-20/22, genome-linked virus protein (VPg), proteinase and polymerase (Sosnovtsev et al., 2006). NoVs are divided into five genogroups (GI-V) based on their genomic composition (Zheng et al., 2006). Human NoVs are classified into GI, GII and GIV, whereas bovine and murine NoVs (MNVs) cluster respectively into GIII and GV. Other NoVs detected in animals constitute distinct genotypes in GII and GIV: porcine NoVs belong to GII and NoVs detected in a lion cub and young dogs cluster into GIV (Martella et al., 2007(Martella et al., , 2008.Little is known about human NoV biology, due to the lack of a regular cell-culture system or small-animal model for human NoVs. MNVs constitute a substitute for the in vitro study of human and other animal NoVs , as it is possible for them to be grown in murine macrophages and dendritic cell lines. Viruses can evolve rapidly due to small-scale mutations and recombination. Genetic recombination enables the creation of new combinations of genetic materials, generating more dramatic genomic changes than point mutations. This phenomenon has been described for a large number of RNA viruses (Lai, 1992). Predictive recombination tools together with similarity plots between putative recombinant genomes and the suspected parental genomes have suggested rec...

show abstract

Molecular characterization of calicivirus strains detected in outbreaks of gastroenteritis in Argentina

Cited by 35 publications

References 49 publications

Genetic diversity of noroviruses in Brazil

Genetic diversity of noroviruses in Brazil

Norovirus GII.17 Predominates in Selected Surface Water Sources in Kenya

Experimental evidence of recombination in murine noroviruses

Contact Info

Product

Resources

About