Molecular characterization of bromeliads from northeast Brazil

Abstract

ABSTRACT. Bromeliaceae is an important botany family that includes many species with economic value; demand for members of this family is increasing. However, illegal collection frequently occurs, drastically reducing the species populations; thus, it is necessary to collect and store Bromeliaceae genetic material. In this study, we identified and quantified genetic variability of the Bromeliad family using dominant markers to create the first Germplasm Bank in the northeast region of Brazil. Molecular tools were used to characterize the collected accessions. The combination of 11 inter-simple sequence repeats and 13 random amplified polymorphic DNA markers were used to detect the genetic variability of wild bromeliad accessions.

Caracterización morfológica y variabilidad genética en genotipos de los géneros Vriesea y Guzmania (Bromeliaceae) con potencial ornamental

“…La diversidad genética en la familia Bormeliaceae ha sido previamente estudiada a través del uso de marcadores moleculares. Vieira et al (2014) identificaron la variabilidad genética cuantificada de la familia Bromeliaceae utilizando marcadores dominantes para crear el primer banco de germoplasma en la región noreste de Brasil. Con el objetico anterior se utilizaron 13 marcadores de ADN polimórficos amplificados aleatoriamente para detectar la variabilidad genética de las accesiones de bromelias silvestres.…”

“…En la familia Bromeliaceae, son escasos los estudios donde se utilicen marcadores morfológicos para evaluar diversidad genética. La mayoría de los estudios genéticos han sido enfocados en el uso de técnicas moleculares como RAPD (Vieira et al, 2014) y microsatélites (Neri et al, 2017).…”

Genetic diversity and fragmentation of Aspidosperma quebracho-blanco (Apocynaceae) natural habitats, conservation issues in Chaco forest and savanna biomes

“…Thirty-three ISSR primers were tested, 30 of which were developed by the University of British Columbia Biotechnology Laboratory (UBCBL), named UBC primers (NAPS, 2019), and three others by Al-Otaibi (2008), named Primers Numbers 7, 12 and 16 (Table 2). (Ribeiro et al, 2013;Vieira et al, 2014). Amplified products were visualized on SyberGold™ stained 1.5% agarose gel (Invitrogen ©, Thermo Fisher Scientific, Waltham, Massachusetts) and 1X TBE under a 120mA direct current.…”