Molecular characterization of bovine tuberculosis strains in two slaughterhouses in Morocco

Abstract

BackgroundBovine tuberculosis (BTB) is caused by Mycobacterium bovis, which belongs to the Mycobacterium tuberculosis complex. Mycobacterium bovis have been described to be responsible of most cases of bovine tuberculosis. Although M. tuberculosis, M. africanum and non-complex mycobacteria were isolated from cattle.In Morocco, so far, no molecular studies were conducted to characterize the strains responsible of BTB. The present study aims to characterize M. bovis in Morocco.The present study was conducted in slaughterhouses in Rabat and El Jadida. Samples were collected from 327 slaughtered animals with visible lesions suggesting BTB.ResultsA total of 225 isolates yielded cultures, 95% (n = 215) of them were acid-fast (AF). Sixty eight per cent of the AF positive samples were confirmed as tuberculous mycobacteria (n = 147), 99% of these (n = 146) having RD9 and among the latter, 98% (n = 143) positive while 2% (n = 3) negative for RD4A total of 134 samples were analyzed by spoligotyping of which 14 were in cluster and with 41 different spoligotypes, ten of them were new patterns (23%). The most prevalent spoligotypes were SB0121, SB0265, and SB0120, and were already identified in many other countries, such as Algeria, Spain, Tunisia, the United States and Argentina.ConclusionThe shared borders between Algeria and Morocco, in addition to the previous importation of cattle from Europe and the US could explain the similarities found in M. bovis spoligotypes. On the other hand, the desert of Morocco could be considered as an efficient barrier preventing the introduction of BTB to Morocco from West Central and East Africa. Our findings suggest a low level endemic transmission of BTB similar to other African countries. However, more research is needed for further knowledge about the transmission patterns of BTB in Morocco.

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

“…This DNA extraction method has been reported by our laboratory and others (Lamine‐Khemiri et al, ; Shimizu et al, ; Yahyaoui‐Azami et al, ; Zumárraga et al, ).…”

“…tory and others(Lamine-Khemiri et al, 2013;Shimizu et al, 2014;Yahyaoui-Azami et al, 2017;Zumárraga et al, 2013).…”

A first insight into genetic diversity of Mycobacterium bovis isolated from extrapulmonary tuberculosis patients in South Tunisia assessed by spoligotyping and MIRU VNTR

“…The most detected spoligotype in our study was SB0120 (26.9%) which is also the most common in humans in Italy (63.8%) [31], France (69%) [32], Germany (20%) [33] compared to 1% in the United Kingdom [34]. SB0120 is also detected in cattle in some European countries like France [35], Italy [30], Portugal [30, 36], Spain [37], and Germany [33] as well as in neighboring countries (Algeria and Morocco) [38, 39].…”

“…bovis cases in France [32], in Spain (10.5% to 13.1%) [40; 41], in London, in the southeast of England (6%) [29], in UK (2%) [34] and in Italy (2%) [31]. SB0121 and SB0134 were previously detected in animal isolates all over the world [30] including neighboring countries such as Algeria [38] and Morocco [39]. Interestingly, this is the first study to identify SB2025 and SB1200 in human M .…”

Comparison of Demographic, Laboratory Parameters and Clinical Outcomes Among Tuberculosis Patients With and Without Hypertension in Kanpur at Tertiary Care Teaching Hospital

“…Tuberculosis is an infectious disease and caused by bacillus Mycobacterium tuberculosis (MTB) which is a part of a complex of organisms including Mycobacterium bovis (reservoir cattle) and Mycobacterium africanum (reservoir human). It typically affects the lungs (pulmonary TB) but can also affect other sites (extrapulmonary TB) 1 . Mycobacterium tuberculosis is an obligate, pathogenic, rod-shaped and non-motile organism belonging to the family Mycobacteriaceace.…”