Molecular Characterization of Bovine Rotaviruses in Pakistan

Mukhtar, Nadia; Yaqub, Tahir; Masood, Ambreen; Javed, Hasnain; Nazir, Jawad; Aslam, Asim; Ali, Akhtar; Javed, Maryam; Nadeem, Asif; Hussain, Tanveer; Tahir, Zarfashan; Aslam, Hassaan Bin

doi:10.5812/jjm.41001

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Basera et al (2010) detected 10 (10/13, 76.9%) samples positive for VP6 antigen. Mukhtar et al (2016) reported the amplification of VP6 gene (33.3%) in 04 Presence of Human G Rotavirus Genotypes Amongst Bovine Calves and its Combination with P Genotypes diarrheic samples out of total twelve ELISA positive samples. Singh et al (2015) reported the amplification of VP6 gene in 12/ 100 (12%) fecal samples from Mathura region.…”

Section: Resultsmentioning

confidence: 97%

Presence of Human G Rotavirus Genotypes Amongst Bovine Calves and its Combination with P Genotypes

Gupta¹,

Nayak²,

Swamy³

et al. 2021

IJAR

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Background: Bovine Rotavirus is one of the most important viral etiological agent responsible for causing neonatal diarrhea incurring severe economic loss to farmers. The presence of large genome size, segmented nature and absence of proof reading activity of RNA polymerase leads to frequent reassortment and thus emergence of new G and P types with ability of interspecies transmission. Methods: During an epidemiological study (July 2016 to July 2019) 200 diarrheic fecal samples were screened for Bovine Rotavirus A using ELISA and RNA-PAGE. Further, twenty two positive samples for RVA were subjected to molecular detection for VP6, VP4 and VP7 genes. Result: Ten (20/200) and 11(22/200) percent diarrheic fecal samples were found positive using ELISA and RNA-PAGE respectively. Twenty samples found positive in ELISA were also found positive in RNA-PAGE. Amongst which 22 (100%) samples were found positive for VP6, while 15 (68.18%) samples showed amplification for VP4 and VP7 gene. All Rotavirus A positive samples were genotyped by multiplex RT-PCR assay. G1G3 was found to be most predominant (53.33%) followed by G3 (26.66%), while one sample each showed the presence of G1G5 and G3G8 (6.66%). Ten samples showed mixed genotype (66.66%). One sample was non typeable (6.66%). Among the P types, P[11] was the most predominant (73.33%), while one sample each showed the presence of P[5] and P[5]P[11] (6.66%) and 02 samples were non typeable (13.33%). The G and P genotype combination determined in 12 samples were as follows; G3P [11] found in two samples (16.66%), G3P[5] in 01(08.33%), G1G5P[11] in 01(08.33%), G1G3P[11] in 07 (58.33%), while 01 (08.33%) sample had mixed genotype G1G3P[5]P[11] combination.

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Section: Resultsmentioning

confidence: 97%

Presence of Human G Rotavirus Genotypes Amongst Bovine Calves and its Combination with P Genotypes

Gupta¹,

Nayak²,

Swamy³

et al. 2021

IJAR

View full text Add to dashboard Cite

show abstract

“…The researcher Ade et al, (2019) reported that only one fecal sample was positive for amplification of VP4 gene out of 35 LAT positive samples might be due to the presence of inhibitory substances in the fecal samples. The researcher Mukhtar et al, (2016) reported that five fecal samples showed the specific amplification for VP4 gene by RT-PCR out of 12 ELISA positive samples obtained from screening of 200 fecal samples. The present study delineated that the PCR positivity is varied might be due to inhibitory factors or mismatched with 3' end of primer binding sites in Indian rotaviral strains.…”

Section: Detection Of Bovine Rotaviruses By Rt-pcr With Group Specific Vp6 Genementioning

confidence: 99%

Molecular Detection of Group A Rotaviruses from the Bhandara and Chandrapur District of Maharashtra State

Kadam¹,

Tembhurne²,

Bonde³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Molecular Characterization of Bovine Rotaviruses in Pakistan

Cited by 2 publications

References 17 publications

Presence of Human G Rotavirus Genotypes Amongst Bovine Calves and its Combination with P Genotypes

Presence of Human G Rotavirus Genotypes Amongst Bovine Calves and its Combination with P Genotypes

Molecular Detection of Group A Rotaviruses from the Bhandara and Chandrapur District of Maharashtra State

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