Molecular characterization of Boeremia strasseri the causal agent of black stems and rhizomes rot of peppermint

Abstract

The molecular characterization of Boeremia strasseri (Moesz) Gruyter et Verkley (basionym Phoma strasseri Moesz) isolates from peppermint crops grown in south-eastern and central Poland was studied using the Random Amplified Polymorphic DNA (RAPD) -PCR technique. Tests were performed using randomly selected primers. DNA profiles obtained using five primers proved to be useful in determining the genetic variability among B. strasseri genotypes. Molecular analysis of four loci: (i) rDNA internal transcribed spacer region (ITS1, 5.8S, ITS2); (ii) LSU (partial large subunit DNA 28S); (iii) tub 2 (gene region of βtubulin); (iv) act (gene region of gamma-actin) proved that the actin gene is the most suitable DNA barcode for the accurate and rapid identification B. strasseri species.

Effects of lime nitrogen on the fungi community of tobacco soil and its correlation analysis

“…In addition, it is worth noting that the relative abundance of Boeremia in the lime nitrogen group and the lime nitrogen with microbial biocontrol agent group was higher than that of the control group at maturity period. Research showed that Boeremia exigua was the pathogen of fruit spot of okra [47] and Boeremia strasseri could induce black stem and rhizomes rot of peppermint [48]. During this period, AFe had a significantly negative correlation with Boeremia, while AK and protease activity had a significantly positive correlation with Boeremia.…”

Phoma on Medicinal and Aromatic Plants

“…Melouk and Horner (1973) pointed to the role of β-glucosidase and pectinolytic enzymes in the process of B. strasseri pathogenesis, and they showed that the optimal pH for the activity of β-glucosidase is within the range from 4.5 to 5.5. Zimowska and Targoński (2015) proved that pectinolytic enzymes of B. strasseri show the greatest activity at the temperature above 30 °C, which together with the eurythermal character of the fungus suggests that increased occurrence of black rot of stems and rhizomes should be taken into consideration during hot periods of vegetation (Fig. 3.2).…”

“…In both processes the most important barriers to overcome are pectin compounds building the cell walls and the middle lamella (Gummadi and ). The enzymes produced by B. strasseri which are responsible for tissue maceration of mint stems and rhizomes mainly concerned β-glucosidase, the enzyme which catalyzes the reactions of cellobiose decomposition to glucose (Zimowska and Targoński 2015). Melouk and Horner (1973) pointed to the role of β-glucosidase and pectinolytic enzymes in the process of B. strasseri pathogenesis, and they showed that the optimal pH for the activity of β-glucosidase is within the range from 4.5 to 5.5.…”

The Genus Phoma: What We Know and What We Need to Know?

“…Internal transcribed spacer regions 1 and 2 and 5.8S nrDNA (ITS), partial large subunit 28S nrDNA region (LSU) and β-tubulin gene (TUB) (Woudenberg et al 2009;Aveskamp et al 2009aAveskamp et al , 2010 in combination with morphological characteristics have been routinely used to resolve phylogenetic relationships in Phoma. RNA polymerase II gene (RPB2) has also been used for several species (Chilvers et al 2009), while actin (ACT) (Aveskamp et al 2009b;de Gruyter et al 2012;Zimowska et al 2018), chitin synthase 1 (CHS-1) (de Gruyter et al 2012) region and the translation elongation factor gene (TEF1) (Irinyi et al 2009) have been applied for Boeremia exigua (Desm.) Aveskamp, Gruyter & Verkley, Boeremia strasseri (Moesz) Gruyter et Verkley and Leptosphaeria doliolum (Pers.)…”