Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil

Cabrine-Santos, Marlene; Moura, Renata Gregório Franco; Pedrosa, André Luiz; Correia, Dalmo; Oliveira-Silva, Márcia Benedita de

doi:10.1590/0037-8682-0305-2021

Cited by 5 publications

(24 citation statements)

References 39 publications

(64 reference statements)

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“…Frequently detected in humans across a broad geographic range, ST4 is likely a significant concern for public health [11,19,41]. In contrast, infections with ST5 have been infrequently reported and only from a small number of countries [13,42,43]. Moreover, the potentially zoonotic ST4 and ST5 have been recently reported in patients in several provinces of China, including regions surveyed in the present study [42,[44][45][46].…”

Section: Resultsmentioning

confidence: 63%

Occurrence and subtyping of Blastocystis in coypus (Myocastor coypus) in China

Liu

Wang

et al. 2022

Parasites Vectors

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Background Blastocystis is an anaerobic unicellular protist frequently detected in the gastrointestinal tracts of humans and animals worldwide. However, the prevalence and subtype distribution of Blastocystis in the coypu (Myocastor coypus) population have not been reported so far. The aim of this study was to determine the prevalence, genetic characteristics, and zoonotic potential of Blastocystis isolates detected in coypus in China. Results A total of 308 fecal samples were collected from coypus in seven regions across China and subsequently examined. Blastocystis was detected in 44 (14.3%) specimens by nested PCR amplification of the small subunit ribosomal rRNA (SSU rRNA) gene. Further DNA sequencing and phylogenetic analyses resulted in the identification of two zoonotic known subtypes, ST4 and ST5, and an unknown subtype. ST4 was the most predominant subtype observed in the samples. ST5 infections were only observed in three coypus. Factors that were associated with prevalence of Blastocystis included age, geographical region and subtype. Interestingly, this is the first report about a potentially novel subtype infecting coypus. Conclusions This is the first comprehensive report of Blastocystis in M. coypus across a wide geographic range of China. A moderate degree of genetic divergence was observed. The presence of zoonotic subtypes in farmed M. coypus suggests that these animals have the potential to transmit blastocystosis to both humans and domestic animals. These findings provide a better understanding of the genetic diversity of Blastocystis in rodents and contribute towards the establishment of efficient blastocystosis control strategies in the investigated areas. Graphical abstract

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Section: Resultsmentioning

confidence: 63%

Occurrence and subtyping of Blastocystis in coypus (Myocastor coypus) in China

Liu

Wang

et al. 2022

Parasites Vectors

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“…The eligibility of 229 full-text articles that passed the title and abstract screening phase was assessed based on preset inclusion and exclusion criteria. Finally, 36 articles [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ] were included in this systematic review.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2 shows the eighteen articles published in Q1 SJR journals [ 50 , 51 , 52 , 54 , 55 , 61 , 63 , 64 , 65 , 70 , 71 , 75 , 76 , 77 , 79 , 80 , 81 , 82 ], the fifteen articles in Q2 SJR journals [ 57 , 58 , 59 , 60 , 62 , 66 , 67 , 68 , 69 , 72 , 73 , 74 , 83 , 84 , 85 ], and the three articles found in Q3 SJR journals [ 53 , 56 , 78 ].…”

Section: Resultsmentioning

confidence: 99%

“…Based on the JBI score rating system tool, thirty-three articles were considered as high-quality scientific papers [ 50 , 51 , 52 , 54 , 55 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ] and only three were of moderate quality [ 53 , 56 , 78 ]. The studies were reported from Argentina [ 50 , 51 ], Bolivia [ 52 , 53 , 54 ], Brazil [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

Section: Resultsmentioning

confidence: 99%

“…The studies were reported from Argentina [ 50 , 51 ], Bolivia [ 52 , 53 , 54 ], Brazil [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ], Chile [ 66 ], Colombia [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ], Ecuador [ 78 ], Honduras [ 79 ], Mexico [ 80 , 81 , 82 , 83 ], Panama [ 84 ], and Peru [ 85 ]. Fifteen studies were conducted on urban citizens [ 51 , 56 , 57 , 58 , 59 , 62 , 64 , 66 , 67 , 69 , 71 , 73 , 74 , 75 , …”

Section: Resultsmentioning

confidence: 99%

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Molecular Prevalence and Subtypes Distribution of Blastocystis spp. in Humans of Latin America: A Systematic Review

Fusaro,

Bernal,

Baldiris-Ávila

et al. 2024

TropicalMed

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Blastocystis spp. are among the few enteric parasites with a prevalence that can reach up to approximately 80% in communities of developing countries. This systematic review updates and summarizes available literature on the molecular prevalence and subtype distribution of Blastocystis spp. in Latin American people. This work follows the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. The literature revised covers from 1 January 2015 to 6 October 2023 in seven different scientific databases, and the material was selected through inclusion and exclusion criteria. According to data found in the 36 selected articles, the prevalence of Blastocystis spp. in Latin America ranged between 5.8% (Bolivian rural communities) and 94.0% (Colombian general public). Generally, genomic DNA was extracted from approximately 200 mg fecal sediments using commercial kits, such as the QIAamp Stool Mini Kit (QIAGEN, Hilden, Germany) or the Norgen Stool DNA Isolation Kit (Norgen Biotek Corporation, Thorold, ON, Canada). Subtype-specific primers (such as the couple of primers BhRDr–RD5) developed from unique sequences of the SSU rRNA gene were applied to Blastocystis subtyping. Ten specific subtypes (STs) were found as well as various mixed infections, and the most circulating Blastocystis STs were in the order ST3, ST1, ST2, and ST4. The most recent data about Blastocystis spp. molecular epidemiology and the STs in communities of Latin America are limited to studies from specific countries. Novel scientific data from the other countries are required to obtain a complete picture and truly understand the distribution and prevalence of Blastocystis spp. and the STs.

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