Molecular characterization of blaESBL–harboring conjugative plasmids identified in multi-drug resistant Escherichia coli isolated from food-producing animals and healthy humans

Juan, Wang; Stephan, Roger; Karczmarczyk, Maria; Yan, Qiongqiong; Hächler, Herbert; Fanning, Séamus

doi:10.3389/fmicb.2013.00188

Cited by 69 publications

(70 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is in agreement with data from previous studies, which demonstrated that bla CTX-M-1 is one of the most prevalent ESBL genes detected in Enterobacteriaceae of broiler origin (45)(46)(47)(48). Also in line with studies performed on broiler farms, the most common replicons encountered in this study were IncI1 and IncFIB (49,50). Moreover, we have found five isolates with the same ESBL gene harbored in two different plasmids.…”

Section: Discussionsupporting

confidence: 82%

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Solà-Ginés

González-López

Cameron-Veas

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

bFlies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence of Escherichia coli strains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the resistant population was performed by different methods: MIC determination, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and phylotyping. The presence of extended-spectrum beta-lactamase (ESBL) genes, their plasmid location, and the mobile genetic elements involved in their mobilization were studied. Additionally, the presence of 35 genes associated with virulence was evaluated. Out of 682 flies captured, 42 yielded ESBL-producing E. coli. Of these isolates, 23 contained bla CTX-M-1 , 18 contained bla CTX-M-14 , and 1 contained bla CTX-M-9 . ESBL genes were associated mainly with the presence of the IncI1 and IncFIB replicons. Additionally, all the strains were multiresistant, and five of them also harbored qnrS. Identical PFGE profiles were found for E. coli isolates obtained from flies at different sampling times, indicating a persistence of the same clones in the farm environment over months. According to their virulence genes, 81% of the isolates were considered avian-pathogenic E. coli (APEC) and 29% were considered extraintestinal pathogenic E. coli (ExPEC). The entrance of flies into broiler houses constitutes a considerable risk for colonization of broilers with multidrug-resistant E. coli. ESBLs in flies reflect the contamination status of the farm environment. Additionally, this study demonstrates the potential contribution of flies to the dissemination of virulence and resistance genes into different ecological niches.

show abstract

Section: Discussionsupporting

confidence: 82%

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Solà-Ginés

González-López

Cameron-Veas

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The virulence and resistant genes found in APEC have been detected on IncFIB plasmids, such as pAPEC-1 (Mellata et al, 2012). All plasmid replicons reported in the current study have been previously detected in E. coli isolates sourced from the environment, other animals and humans (Bortolaia et al, 2010;Garcia-Fernandez et al, 2008;Kluytmans et al, 2013;Lynne et al, 2012;Seni et al, 2016;Wang et al, 2013a;Woodford et al, 2007).…”

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicmentioning

confidence: 78%

“…The IncI1, IncFIB, IncY and IncN plasmids have been identified in poultry previously and were associated with MDR and ESBLs producing E. coli (Bortolaia et al, 2010;Garcia-Fernandez et al, 2008;Kluytmans et al, 2013;Lynne et al, 2012;Seni et al, 2016;Wang et al, 2013a). One of the ESC resistant isolates contained IncY and IncI1 plasmid types, while the other contained IncFIA, IncFIB and IncFrep plasmids.…”

Section: Discussionmentioning

confidence: 99%

“…The frequency of APEC related VGs among E. coli strains vary and VG profile may be influenced by several factors such as geographical locations, season, bird immune status, variation in the sampling sites, different husbandry and vaccination protocols (Johnson et al, 2008b;Qabajah et al, 2014;Wang et al, 2013a). Therefore, APEC VG markers specific to Australian conditions need to be defined in order to better identify APEC and reduce the economic losses associated with the disease in the poultry industry.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

show abstract

“…This establishes a transferability of 28.57% which is almost in concordance with results (30%-80%) of other similar studies. 7,[21][22][23] These two strains and another one (from HiMedia Kit) were further experimented to document the effects of Acriflavine.…”

Section: Discussionmentioning

confidence: 99%

In-Vitro Study on Potential Efficacy of Acriflavine in the Prevention of Conjugational Transfer of Drug Resistance in Bacteria

Das¹,

Chakraborty²,

Ray³

2017

jemds

View full text Add to dashboard Cite

BACKGROUNDAcquisition of new resistance in bacteria is brought into effect by a battery of gene transfer systems, among which conjugation accounts for bulk of horizontal gene transfer. Acriflavine is a DNA-intercalating agent with plasmid curing properties. Since it selectively inhibits plasmid replication, it might have a role in interfering with conjugation.The aim of this study was to observe for any in-vitro inhibitory effect of Acriflavine on horizontal transfer of resistance factors.

show abstract

Molecular characterization of blaESBL–harboring conjugative plasmids identified in multi-drug resistant Escherichia coli isolated from food-producing animals and healthy humans

Cited by 69 publications

References 46 publications

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

In-Vitro Study on Potential Efficacy of Acriflavine in the Prevention of Conjugational Transfer of Drug Resistance in Bacteria

Contact Info

Product

Resources

About