Molecular Characterization of Betasatellite Associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java

Abstract

Ageratum conyzoides is one of the common weeds in Indonesia. The presence of weed can decrease plant yield by resource competition and its role as alternative hosts for pests and diseases, especially begomoviruses transmitted by whitefly. The aim of this research is to detect and characterize the begomovirus-beta satellite in A. conyzoides. A. conyzoides showed severe yellowing symptoms were collected from Magelang, Central Java, Indonesia. Total DNA was extracted and analyzed using PCR method for begomovirus and beta satellite. Begomovirus detection was performed with universal primer Krusty-Homer, resulted a 500 bp DNA fragment. Betasatelit detection performed with specific primer β01/β02, resulted a 1300 bp DNA fragment, indicated presence of a beta satellite associated with the begomovirus. The sequence of begomovirus showed 95% similarity with Tomato Leaf Curl Java Virus (ToLCJaV). The sequence of beta satellites showed 85% similarity with Tomato Leaf Curl Betasatellite (ToLCB). Characterization of beta satellite showed three main characters of beta satellite, TAATATTAC stem-loop structure, Adenine-rich region, and 118 amino acids of putative ORF BC1.

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

“…However, the typical symptom of yellow curly leaves was found in these plant samples (Figure 3). The DNA fragments in the two islands are similar to the Begomovirus DNA in chili plants in Yogyakarta and Central Java (580 bp) (Kandito et al 2019). The size of DNA fragments from chili Begomovirus PCR results in several areas in Indonesia has been previously reported, such as in Java and Sumatra, at 400 bp and in Bali at 912 bp (Fadhila et al 2022;Selangga et al 2019).…”

“…Leguminosae and Solanaceae are the most common Begomovirus hosts (Wilisiani et al 2014;Wang et al 2020;Kwak et al 2022). Weed babadotan (Ageratum conyzoides) (Kandito et al 2019), (Physalis sp) (Lopez-lopez 2014) and Verbesina encelioides, Calotropis procera, Mimosa pudica is the most common alternative host in chili cultivation (Prajapat et al 2014).…”

“…Additionally, Ghosh et al (2019) and Pinheiro-Lima et al (2020) described the Begomovirus host, which is primarily dicotyledonous plants and is spread by whitefly insects (Bemisia tabaci). The signs of a Begomovirus infection on chili plants have been documented in several prior reports as mosaic leaves and yellow color (Kesumawati et al 2020), leaf vein clearing (Kandito et al 2019), thickened leaves (Koeda et al 2018), curly and wrinkled leaves (Noha et al 2014), shortening of the internodes and petioles (Shingote et al 2022) and stunted plants (Kwak et al 2022).…”

“…Ageratum conyzoides and Physalis sp. is the most common alternative host in chili cultivation (Kandito et al 2019;Lopez-Lopez 2014).…”

Begomovirus Associated with Yellow Mosaic Symptom on Eggplant Plant in Bengkulu

“…Daun muda bergejala diambil sebanyak lima lembar, selanjutnya daun dimasukkan ke dalam kantung plastik dan disimpan pada suhu -20 ℃ sebelum diekstraksi DNA totalnya. (Kon et al 2006(Kon et al dan 2007Kandito et al 2019Kandito et al , 2020Kandito et al , 2021. Analisis sikuen DNA dengan program BLAST menunjukkan Begomovirus isolat terung dari Bengkulu memiliki similaritas yang tinggi, sekitar 99% terhadap Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) asal Indonesia, Malaysia dan Thailand (Tabel 1).…”