Molecular Characterization of Apple stem grooving virus Isolated from Talaromyces flavus

Shim, Hye Kyung; Hwang, Kyu Hyon; Shim, Chang Ki; Son, Su Wan; Kim, Dong Giun; Choi, Yong Mun; Chung, Young Jae; Kim, Dae Hyun; Jee, Hyeong Jin; Lee, Suk Chan

doi:10.5423/ppj.2006.22.3.260

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Apple stem grooving virus (ASGV) is a member of the genus Capillovirus and belongs to the family Betaflexiviridae ( 1 ). The virus has a polyadenylated positive-sense single-stranded RNA genome consisting of two overlapping open reading frames (ORFs) that encode a polyprotein and a movement protein (MP) ( 2 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequences of Two Apple Stem Grooving Viruses in Cnidium officinale in Korea

Lee

Kim

et al. 2023

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Complete Genome Sequences of Two Apple Stem Grooving Viruses in Cnidium officinale in Korea

Lee

Kim

et al. 2023

Microbiol Resour Announc

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“…Total RNA was extracted from apple plantlet leaves and RNA quality and concentration were determined following the methodology of Kim et al (2019). The RNA samples were first tested for ASGV presence using RT-PCR with previously used diagnostic primers (Shim et al, 2006). First-strand cDNA was synthesized using 2 μg total RNA, 0.5 μg random primer, 10 mM dNTP, RNase inhibitor (TaKaRa, Shinga, Japan), M-MLV reverse transcriptase (Promega, Madison, WI, USA) in 20 μl volume.…”

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confidence: 99%

Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR

et al. 2022

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Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees.

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Complete sequence of an Apple stem grooving virus (ASGV) isolate from China

et al. 2012

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The complete genome sequence of a Chinese isolate of Apple stem grooving virus (ASGV) was determined to be 6,495 nucleotides long, single-stranded, plus-sense RNA. The viral RNA has two overlapping open reading frames (ORFs): ORF1 and ORF2. Compared with the genome sequences of ASGV isolates available in GenBank, the nucleotide identities ranged from 80.1 to 86.3 %. The amino acid identities of proteins encoded by ORF1 and ORF2 ranged from 79.5 to 86.1 % and 82.0 to 85.9 %, respectively.

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Molecular Characterization of Apple stem grooving virus Isolated from Talaromyces flavus

Cited by 4 publications

References 17 publications

Complete Genome Sequences of Two Apple Stem Grooving Viruses in Cnidium officinale in Korea

Complete Genome Sequences of Two Apple Stem Grooving Viruses in Cnidium officinale in Korea

Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR

Complete sequence of an Apple stem grooving virus (ASGV) isolate from China

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