Molecular characterization of Anaplasma marginale based on the msp1a and msp1b genes

Junsiri, Witchuta; Watthanadirek, Amaya; Poolsawat, Napassorn; Minsakorn, Sutthida; Nooroong, Pornpiroon; Jittapalapong, Sathaporn; Chawengkirttikul, Runglawan; Anuracpreeda, Panat

doi:10.1016/j.vetmic.2021.109236

Cited by 8 publications

(2 citation statements)

References 45 publications

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“…The prevalence of anaplasmosis in dairy cattle in Khon Kaen province by molecular detection was 12.73% (49/385) with a 95% CI of 0.51–1.94 for A. marginale based on the msp4 gene. In addition to msp1a , msp1b , msp2 , and msp5 [ 23 , 31 , 32 ], the msp4 gene was used in this study as a good marker for providing sufficient variation to create a large-scale phylogeographic pattern [ 15 ]. It is useful for epidemiological analysis of anaplasmosis [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand

Arjentinia,

Keomoungkhoun,

Thamrongyoswittayakul

et al. 2024

Vet World

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Background and Aim: Bovine anaplasmosis (BA) is one of the most important diseases of ruminants worldwide, causing significant economic losses in the livestock industry due to the high morbidity and mortality in susceptible cattle herds. Anaplasma marginale is the main causative agent of BA occurring worldwide in tropical and subtropical regions. This study aimed to investigate the first molecular detection and genetic diversity of A. marginale in dairy cattle in Khon Kaen Province, Thailand. Materials and Methods: Blood samples were collected from 385 lactating cows from 40 dairy farms in five districts of Khon Kaen, regardless of age and health status. To detect A. marginale, all DNA preparations were used for molecular diagnosis using a single polymerase chain reaction with the msp4 gene target. A phylogenetic tree was constructed from the msp4 gene sequences using molecular genetic characterization. Genetic diversity was calculated as haplotype diversity, haplotype number, number of nucleotide differences, nucleotide diversity, and average number of nucleotide differences. Results: The overall prevalence of A. marginale was 12.72% (49/385). The highest prevalence (17.19%) was found in Ubolratana district, followed by Muang, Kranuan, Khao Suan Kwang, and Nam Phong districts (14.94%, 14.74%, 13.79%, and 3.70%, respectively). Phylogenetic analysis showed that A. marginale was closely related to isolates from Australia (98.96%), China (99.68%), Spain (99.74%), and the USA (99.63%). Conclusion: The molecular prevalence of BA in dairy cattle is the first to be observed in this area, and the genetic variability with separated clusters shown in the msp4 gene of A. marginale revealed species variation in dairy cattle. This significant genetic diversity contributes to the understanding of the diversity of A. marginale and will be important for the control and prevention of A. marginale in dairy cattle. Keywords: Anaplasma marginale, bovine anaplasmosis, dairy cattle, molecular prevalence.

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Section: Discussionmentioning

confidence: 99%

First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand

Arjentinia,

Keomoungkhoun,

Thamrongyoswittayakul

et al. 2024

Vet World

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“…The cattle blood samples in this work were obtained from previous studies 32 , 51 . They are collected from different areas in the northern and northeastern parts of Thailand.…”

Section: Methodsmentioning

confidence: 99%

RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection

Sutipatanasomboon,

Wongsantichon,

Sakdee

et al. 2024

Sci Rep

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Anaplasma marginale infection is one of the most common tick-borne diseases, causing a substantial loss in the beef and dairy production industries. Once infected, the pathogen remains in the cattle for life, allowing the parasites to spread to healthy animals. Since clinical manifestations of anaplasmosis occur late in the disease, a sensitive, accurate, and affordable pathogen identification is crucial in preventing and controlling the infection. To this end, we developed an RPA-CRISPR/Cas12a assay specific to A. marginale infection in bovines targeting the msp4 gene. Our assay is performed at one moderately high temperature, producing fluorescent signals or positive readout of a lateral flow dipstick, which is as sensitive as conventional PCR-based DNA amplification. This RPA-CRISPR/Cas12a assay can detect as few as 4 copies/μl of Anaplasma using msp4 marker without cross-reactivity to other common bovine pathogens. Lyophilized components of the assay can be stored at room temperature for an extended period, indicating its potential for field diagnosis and low-resource settings of anaplasmosis in bovines.

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Ehrlichia canis: Molecular characterization and genetic diversity based on the p28 and trp36 genes

Poolsawat

Noorong

Junsiri

et al. 2023

Research in Veterinary Science

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Molecular characterization of Anaplasma marginale based on the msp1a and msp1b genes

Cited by 8 publications

References 45 publications

First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand

First report on the molecular detection and genetic diversity of Anaplasma marginale in healthy dairy cattle in Khon Kaen province, Thailand

RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection

Ehrlichia canis: Molecular characterization and genetic diversity based on the p28 and trp36 genes

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