Molecular characterization of an exopolysaccharide from a probiotic Lactobacillus plantarum MTCC 9510 and its efficacy to improve the texture of starchy food

Abstract

The weight average molecular weight (Mw), number average molecular weight (Mn) and size average molecular weight (Mz) of an exopolysaccharide from probiotic Lactobacillus plantarum MTCC 9510 was found to be 2.68×10 5 Da, 2.55×10 5 Da and 2.83×10 5 Da, respectively by Gel permeation Chromatography employing the third order polynomial model. The polydispersity index (Mw/Mn) of the polysaccharide was obtained as 1.05. The exopolysaccharide and the starch-exopolysaccharide hydrocolloid exhibited a non-Newtonian and pseudo-plastic behaviour with improvement in the texture of starch containing food by preventing syneresis.

Lactobacillus plantarumMTCC 9510 supplementation protects from chronic unpredictable and sleep deprivation-induced behaviour, biochemical and selected gut microbial aberrations in mice

“…). Furthermore, this strain produces an exopolysaccharide (Ismail and Nampoothiri ), which is well characterized (Ismail and Nampoothiri ) and exhibits anticancer property (Ismail and Nampoothiri ).…”

“…The rational for selecting L. plantarum MTCC 9510 for this study is due to its probiotic attributes which are well established (Aswathy et al 2008). Furthermore, this strain produces an exopolysaccharide (Ismail and Nampoothiri 2010), which is well characterized (Ismail and Nampoothiri 2013) and exhibits anticancer property (Ismail and Nampoothiri 2014).…”

Isolation, identification, and potential probiotic characterization of one Lactococcus from Kefir grain

“…After the broth was centrifuged at 10,000×g for 30 min, a certain volume of supernatant was taken to dialysis through 10 kDa membrane against distilled water at 4 o C for 72 h with 2-4 changes per day. EPS production of the strain was determined by phenolsulphuric acid method (11) until no single sugar was detected in distilled water.…”

OPTIMAL CONDITIONS FOR EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus plantarum T10

“…In the study of Ismail and Nampoothiri [11], the initial cell density of 10 9 CFU/mL was the best condition for L.plantarum MTCC 9510 to synthesize EPS. In our study, the appropriate initial cell density was at 10 6 CFU/mL.…”

“…Li Zhang et al [1] found that L. plantarum C88 was able to produce 69 mg/L EPS in MRS medium supplemented with 2 % glucose. In other study, lactose was chosen to investigate the EPS production of L. plantarum MTCC 9510 [11].…”