Molecular characterization of an ABA insensitive 5 orthologue in Brassica oleracea

Zhou, Xiaona; Yuan, Feifei; Wang, Mengyao; Guo, Aizhen; Zhang, Yanfeng; Xie, Chang Gen

doi:10.1016/j.bbrc.2012.12.023

Cited by 30 publications

(39 citation statements)

References 26 publications

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“…Additionally, application of ABA, drought, salt and osmotic stress activated BolABI5 expression and indicated its role in abiotic stress adaptation. Similar to AtABI5, BolABI5 activates expression of target genes through binding to ABRE elements present in their promoters (Zhou et al, 2013). BolABI5 possesses the ability to interact with BolOST1, a homolog of SnRK2.6/OST1.…”

Section: Function Of Abi5 Orthologs In Dicot and Monocot Plants Durinmentioning

confidence: 99%

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

2016

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ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the regulation of the expression of genes that contain the ABSCISIC ACID RESPONSE ELEMENT (ABRE) motif within their promoter region. The regulated targets include stress adaptation genes, e.g., LEA proteins. However, the expression and activation of ABI5 is not only dependent on the core ABA signaling. Many transcription factors such as ABI3, ABI4, MYB7 and WRKYs play either a positive or a negative role in the regulation of ABI5 expression. Additionally, the stability and activity of ABI5 are also regulated by other proteins through post-translational modifications such as phosphorylation, ubiquitination, sumoylation and S-nitrosylation. Moreover, ABI5 also acts as an ABA and other phytohormone signaling integrator. Components of auxin, cytokinin, gibberellic acid, jasmonate and brassinosteroid signaling and metabolism pathways were shown to take part in ABI5 regulation and/or to be regulated by ABI5. Monocot orthologs of AtABI5 have been identified. Although their roles in the molecular and physiological adaptations during abiotic stress have been elucidated, knowledge about their detailed action still remains elusive. Here, we describe the recent advances in understanding the action of ABI5 in early developmental processes and the adaptation of plants to unfavorable environmental conditions. We also focus on ABI5 relation to other phytohormones in the abiotic stress response of plants.

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Section: Function Of Abi5 Orthologs In Dicot and Monocot Plants Durinmentioning

confidence: 99%

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

2016

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“…Group A bZIP proteins contain three conserved N-terminal (C1-C3) domains and one conserved C-terminal (C4) domain, each of which harbors putative phosphorylation sites (Furihata et al, 2006;Fujita et al, 2009;Zhou et al, 2013). The three conserved motifs in the N-terminal domains of ABI5 and ABSCISIC ACID-RESPONSIVE ELEMENT BINDING PROTEIN1 are phosphorylated in response to ABA (Lopez-Molina et al, 2002;Furihata et al, 2006).…”

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confidence: 99%

SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

et al. 2015

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Abscisic acid (ABA) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4, two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast (Saccharomyces cerevisiae), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5. PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA-responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA-insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis (Arabidopsis thaliana).

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“…For the oomycete Phytophthora infestans, Picdc14 appears to be functionally equivalent to cdc14 of S. cerevisiae based on its complementation of the cdc14-1 ts mutation (Ah Fong and Judelson 2003). In addition, Brassica oleracea ABI5 can rescue the ABA-insensitive phenotype of abi5-1 during seed germination (Zhou et al 2013). Overall, the strictly biotrophic parasitism of rust fungi depend on the host's living tissue for their development, proliferation and reproduction (Feau et al 2007), which has resulted in the lack of a stable transformation system for M. larici-populina.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a Hog1-type MAPK, MlpHog1, from Melampsora larici-populina

et al. 2016

Phytoparasitica

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The obligate biotrophic fungus Melampsora larici-populina (Mlp) causes devastating poplar foliar rust disease. In pathogens, the Hog1-type mitogen-activated protein kinase (MAPK) gene is involved in both pathogenesis and the response to various stresses, including osmotic stress. However, there are few reports on its role in M. larici-populina. In the present study, we identified and characterized the Hog1-type MAPK gene, MlpHog1, in M. larici-populina. The MlpHog1 protein consists of 356 amino acids and contains a conserved TGY motif. The function of MlpHog1 was probed by mutant complementation studies. Expression of MlpHog1 could partially complement the osmoregulation, sexual reproduction and pathogenicity defects of the Fusarium graminearum Hog1 mutant and largely rescued the response of the mutant to cytoplasm membrane and oxidative stresses. These observations suggest that MlpHog1 may play a role in the infectious growth of M. larici-populina and the response of the fungus to various environmental stresses.

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Molecular characterization of an ABA insensitive 5 orthologue in Brassica oleracea

Cited by 30 publications

References 26 publications

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

The Role and Regulation of ABI5 (ABA-Insensitive 5) in Plant Development, Abiotic Stress Responses and Phytohormone Crosstalk

SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5

Molecular characterization of a Hog1-type MAPK, MlpHog1, from Melampsora larici-populina

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