Molecular characterization of Aminoglycosoide and Tetracycline resistant Salmonella isolates causing new born ruminants diarrhea

Abstract

Ten isolates of Salmonella species were obtained from sporadic cases of profuse diarrhea in new born ruminants from El-Menofiya and El-Kalubia Governorates in Egypt. These isolates were as follow 7 isolates from calves (Salmonella Typhimurium, Salmonella Enteritidis,two were Salmonella Saintpaul, two were Salmonella Langeveld and Salmonella Havana) ,two isolates from lambs (Salmonella Typhimurium, Salmonella Bardo) and one isolate from goat-kids(Salmonella Enteritidis). Salmonella isolates resistance to aminoglycosoides was 0.00% for Amikacin, 30% for tobramycin and 50% for streptomycin. Susceptibility of isolates to tetracycline was 50% for doxycycline. Results showed a high incidence of aminoglycosoides resistance gene aadB in 100% of the isolates while aadA2 genes in 40% of the isolates. Incidence of tetracycline resistance genes was 0.00% for tetA(B) and 80% for tetA(A). The difference between the results of this study and those from other regions in Egypt necessitate a complete survey overall the country to make a complete and clear map of salmonella servoars, their antibiotics susceptibility and in molecular characterization of resistance determinants in each region.

Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt

“…According to Table 3, S. Typhimurium and S. Enteritidis were the most frequent serotypes identified in sheep feces. Similar results were reported in previous Egyptian studies [12,14]. S. Heidelberg which was isolated from sheep feces in the present study, has been previously isolated from human stool samples in Zimbabwe [27] and poultry workers in Egypt [28], reflecting its probable zoonotic importance.…”

“…This was in agreement with the findings of other studies conducted in Ethiopia [16,17] and Riyadh, Saudi Arabia [18]. In another study carried out in Egypt, the isolation rate of Salmonella from sheep feces (3.6%) was similar to that from goat feces (3.3%) [12]. Moreover, a higher isolation rate in goats had been recently reported in the USA (10.3%) [19].…”

“…Similar to our results from goats, S. Enteritidis had been isolated in previous studies from goat feces in Egypt [12,13]. Interestingly, in later study, none of our identified serotypes was detected in their study, except two isolates of S. Enteritidis (0.39%) that were identified in diarrheic sheep feces in Giza governorate in Egypt [13].…”

“…carriage in sheep and goats are available. Few previous studies in Egypt have documented the occurrence of Salmonella in diarrheic sheep and goats only, and/or restricted to kids and lambs [12][13][14]; however, none of them have focused on Salmonella prevalence in both diarrheic and apparently healthy sheep and goats and its relationship with Salmonella carriage in human contacts. Therefore, this study aimed to determine the occurrence of Salmonella serovars and their virulence in sheep and goats in the Ismailia and Port-Said governorates in Egypt and the associated risk to humans in the study area.…”