Molecular Characterization of Allergens of Kentucky Bluegrass Pollen

Olsen, Egil; Yang, Ming; Mohapatra, Shyam S.

doi:10.1007/978-1-4757-0235-4_6

Cited by 1 publication

(1 citation statement)

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“…The major goal of cloning allergens is to produce the recombinant proteins in unlimited amounts for use in basic as well as clinical studies. To date, only a few of these allergens have been cloned in plasmid vectors for high-level expression in E. coli, which allow synthesis of the allergenic protein either (i) as a fusion protein, i.e., linked to p-galactosidase (46) or gluthathione S-transferase (37), or (ii) as rALs by itself utilizing vectors such as pKK233 series of expression plasmids (6). Recently, both yeast (9) and baculovirus (49) expression systems have been utilized for the synthesis of certain rALs.…”

Section: Synthesis Of Ralsmentioning

confidence: 99%

In pursuit of the “holy grail”: recombinant allergens and peptides as catalysts for the allergen‐specific immunotherapy

Mohapatra

Cao

et al. 1995

Allergy

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This presentation reviews recent progress in knowledge of (i) molecular biology and immunology of allergens and (ii) the methods for the modulation of allergen-specific immune response, with special emphasis on our grass pollen allergens, particularly Pao p IX AL, used as a model. The last few years have seen tremendous progress in molecular characterization of allergens, leading to synthesis of a number of recombinant allergens. Furthermore, studies of the structure-activity relationship of allergens have led to the delineation of the epitopes of these allergens and of widespread cross-reactivities among diverse allergens. In view of the requirement for extensive pre-clinical studies, the application of the recombinant allergens and epitopes in the clinical realm has just begun. Studies in murine systems suggest that these allergens and/or their epitopic peptides downregulate allergen-specific immune responses de novo. The potential and drawbacks of the recombinant allergens and peptides for improving the existing immunotherapeutic approaches and for developing new approaches to specific immunotherapy are discussed.

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Section: Synthesis Of Ralsmentioning

confidence: 99%