Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland

Jakubowska, Agata; Oers, Monique M. van; Ziemnicka, J.; Lipa, Jerzy J.; Vlak, Just M.

doi:10.1016/j.jip.2005.06.010

Cited by 17 publications

(31 citation statements)

References 26 publications

(33 reference statements)

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“…Three European isolates, a Polish, a French, and an English (Oxford) isolate of Agrotis segetum nucleopolyhedrovirus (AgseNPV), were previously described [16][17][18]. Biochemical and DNA restriction endonuclease analyses of the Polish isolate, which is also designated AgseNPV-A, were found to be significantly different from those of the English and French isolates [16,17,19]. The French and English isolates are actually identical and were designated as AgseNPV-B (Oxford strain) [19].…”

Section: Introductionmentioning

confidence: 99%

The genome sequence of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) reveals a new baculovirus species within the Agrotis baculovirus complex

2014

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The genome of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) was completely sequenced and compared with whole genome sequences of the Agrotis segetum nucleopolyhedrovirus A (AgseNPV-A) and Agrotis ipsilon nucleopolyhedrovirus (AgipNPV). The AgseNPV-B genome is 148,981 bp in length and encodes 150 putative open reading frames. AgseNPV-B contains two copies of the gene viral enhancing factor (vef), making the Agrotis nucleopolyhedroviruses and A. segetum granulovirus (AgseGV) very rich in vef in comparison to other baculoviruses. Genome alignments of AgseNPV-B, AgseNPV-A and AgipNPV showed a very high genome co-linearity interspersed with variable regions, which are considered as putative sites of genomic recombination. Phylogenetic analyses revealed that all three viruses are distinct. However, AgseNPV-B is more closely related to AgipNPV suggesting that both viruses are at an early stage of phylogenetic divergence. It is proposed that AgseNPV-B belongs to a third Alphabaculovirus species of the Agrotis baculovirus complex. The Agrotis exclamationis nucleopolyhedrovirus (AgexNPV) shared high nucleotide sequence identities with AgseNPV-B, suggesting it is actually an AgseNPV-B isolate.

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Section: Introductionmentioning

confidence: 99%

The genome sequence of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) reveals a new baculovirus species within the Agrotis baculovirus complex

2014

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“…AgseNPV has a wider host range including Agrotis ipsilon, Agrotis exclamationis, Agrotis puta, Noctua comes, Peridroma saucia, Xestia sexstrigata and Xestia xanthographa (Bourner & Cory, 2004). However, the virus used to determine the host range of AgseNPV is different from the sequenced Polish AgseNPV isolate (Jakubowska et al, 2006) used in the present work and may represent a different virus species (Jakubowska et al, 2005). …”

Section: Introductionmentioning

confidence: 78%

“…AgseNPV used in this study was isolated in 1975 from A. segetum larvae collected in cabbage crops in Poland (Jakubowska et al, 2005). The virus was freshly amplified in L2 larvae of a current laboratory culture of A. segetum.…”

Section: Methodsmentioning

confidence: 99%

Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed

Jakubowska

Lynn

Herrero

et al. 2009

Journal of General Virology

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Given the high similarity in genome content and organization between Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) and Agrotis segetum nucleopolyhedrovirus (AgseNPV), as well as the high percentages of similarity found between their 30 core genes, the specificity of these NPVs was analysed for the respective insect hosts, S. exigua and A. segetum. The LD 50 for AgseNPV in second-instar A. segetum larvae was 83 occlusion bodies per larva and the LT 50 was 8.1 days. AgseNPV was orally infectious for S. exigua, but the LD 50 was 10 000-fold higher than for SeMNPV. SeMNPV was not infectious for A. segetum larvae when administered orally, but an infection was established by injection into the haemocoel. Bypassing midgut entry by intrahaemocoelic inoculation suggested that the midgut is the major barrier in A. segetum larvae for infection by SeMNPV. Delayed-early genes of SeMNPV are expressed in the midgut of A. segetum larvae after oral infections, indicating that the virus is able to enter midgut epithelial cells and that it proceeds through the first phases of the infection process. The possible mechanisms of A. segetum resistance to SeMNPV in per os infections are discussed.

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“…In addition, both viruses were shown histologically to infect the same insect tissues of A. segetum larvae (17). However, in that study, it was not distinguished whether the virus isolate used was AgseNPV-A or AgseNPV-B, since this nomenclature was later introduced (10). It is not known if there is a reciprocal influence of AgseGV and AgseNPV-B in coinfected larvae and, if so, which kind of effect occurs and if this effect is of a functional and economic benefit when used as a pest control agent.…”

mentioning

confidence: 97%

“…Two different alphabaculovirus isolates, Agrotis segetum nucleopolyhedrovirus A (AgseNPV-A; also called the Polish isolate) and A. segetum nucleopolyhedrovirus B (AgseNPV-B; also termed the Oxford isolate) (9)(10)(11), as well as the betabaculovirus Agrotis segetum granulovirus (AgseGV) (12), were isolated and characterized from A. segetum larvae. AgseGV was tested extensively as a biocontrol agent for the control of A. segetum in the field (13)(14)(15)(16), and AgseNPV-B has shown its potential as a biocontrol agent under laboratory conditions (13).…”

mentioning

confidence: 99%

Mortality of Cutworm Larvae Is Not Enhanced by Agrotis segetum Granulovirus and Agrotis segetum Nucleopolyhedrovirus B Coinfection Relative to Single Infection by Either Virus

Wennmann

Köhler

Alletti

et al. 2015

Appl Environ Microbiol

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Mixed infections of insect larvae with different baculoviruses are occasionally found. They are of interest from an evolutionary as well as from a practical point of view when baculoviruses are applied as biocontrol agents. Here, we report mixed-infection studies of neonate larvae of the common cutworm, Agrotis segetum, with two baculoviruses, Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV). By applying quantitative PCR (qPCR) analysis, coinfections of individual larvae were demonstrated, and occlusion body (OB) production within singly infected and coinfected larvae was determined in individual larvae. Mixtures of viruses did not lead to changes in mortality rates compared with rates of singlevirus treatments, indicating an independent action within host larvae under our experimental conditions. AgseNPV-B-infected larvae showed an increase in OB production during 2 weeks of infection, whereas the number of AgseGV OBs did not change from the first week to the second week. Fewer OBs of both viruses were produced in coinfections than in singly infected larvae, suggesting a competition of the two viruses for larval resources. Hence, no functional or economic advantage could be inferred from larval mortality and OB production from mixed infections of A. segetum larvae with AgseNPV-B and AgseGV.A large number of baculoviruses isolated from the larvae of species of the insect orders Lepidoptera, Diptera, and Hymenoptera have been described (1). According to their occlusion body (OB) morphology, baculoviruses are distinguished into two morphological groups: the granuloviruses (GVs) with a single virion in an ovocylindrical OB (granule) and the nucleopolyhedroviruses (NPVs) with a few to many virions in a polyhedral OB (polyhedron) (2). Taxonomically, the Baculoviridae family is subdivided into four genera, the Alpha-, Beta-, Gamma-, and Deltabaculovirus genera (2, 3). Alpha-and betabaculoviruses comprise lepidopteran-specific NPVs and GVs, respectively. Many lepidopteran species are susceptible to baculoviruses from different species. Even simultaneous infections, so-called coinfections or mixed infections, of two NPVs (4), two GVs (5), and NPVs and GVs (6, 7) have been observed.Larvae of the turnip moth, Agrotis segetum (Denis & Schiffermüller) (Lepidoptera: Noctuidae), so-called common cutworms, are important agricultural pests (8). Two different alphabaculovirus isolates, Agrotis segetum nucleopolyhedrovirus A (AgseNPV-A; also called the Polish isolate) and A. segetum nucleopolyhedrovirus B (AgseNPV-B; also termed the Oxford isolate) (9-11), as well as the betabaculovirus Agrotis segetum granulovirus (AgseGV) (12), were isolated and characterized from A. segetum larvae. AgseGV was tested extensively as a biocontrol agent for the control of A. segetum in the field (13-16), and AgseNPV-B has shown its potential as a biocontrol agent under laboratory conditions (13).Both viruses were found to infect A. segetum larvae simultaneously (17), but little is known about possible in...

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Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland

Cited by 17 publications

References 26 publications

The genome sequence of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) reveals a new baculovirus species within the Agrotis baculovirus complex

The genome sequence of Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) reveals a new baculovirus species within the Agrotis baculovirus complex

Host-range expansion of Spodoptera exigua multiple nucleopolyhedrovirus to Agrotis segetum larvae when the midgut is bypassed

Mortality of Cutworm Larvae Is Not Enhanced by Agrotis segetum Granulovirus and Agrotis segetum Nucleopolyhedrovirus B Coinfection Relative to Single Infection by Either Virus

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