Molecular characterization of Aedes, Culex, Anopheles, and Armigeres vector mosquitoes inferred by mitochondrial cytochrome oxidase I gene sequence analysis

Anoopkumar, A. N.; Puthur, Sreedev; Rebello, Sharrel; Aneesh, Embalil Mathachan

doi:10.2478/s11756-019-00231-0

Cited by 18 publications

(12 citation statements)

References 40 publications

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“…5,5,23,25,26 Altogether, the present status of vector control research urges us to find out cost-effective, environmentfriendly, target-specific and biodegradable natural insecticides against Culex quinquefasciatus mosquitoes. 8,24 Previously, 15 reported on the entomocidal potential of five different plants such as Achyranthes aspera, Chenopodium murale, Trianthema portulacastrum, Convulvulus arvensis, and Tribulus terrestris against Culex quinquefasciatus. In agreement with their findings, 20 reported the extensive larvicidal potential of Plectranthus amboinicus with an LC50 of 147.40 mg/L.…”

Section: Discussionmentioning

confidence: 99%

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Rajan

2021

JCD

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Introduction: Plant-based knowledge has been used for generations for personal protection from various mosquito species. The notion of applying such traditional perspectives in vector control research has received extensive attention in recent years. Unlike other common patterns, the present investigation has tried to explore the augmented production of reactive oxygen species (ROS) in response to Stachytarpheta jamaicensis exposure with special inference on larvicidal potential, mode of action of phytochemical compounds, and oxidative stress. Methods: The larvicidal potential was determined as per the WHO protocol. Ultraviolet-visible spectroscopy was used to determine the excessive production of ROS. GC-MS was employed to characterise the phytochemical constituents. The statistical analysis was done by using SPSS version 24.0.0. Result: The acetone extract has been found to exhibit a maximum range of toxicity in terms of larvicidal potential and reactive oxygen species formation. Among the 40 phytochemical elements characterised, Cyclopropane, 1,1,2,2-Tetramethyl; Phenyl-Acetonitrile; Pyranone; Tetradecene; Neophytadiene; Mome Inositol; Monocrotaline; and Squalene may be responsible for the augmented production of ROS in the Culex quinquefasciatus. Conclusion: The phytochemical elements in Stachytarpheta jamaicensis displayed extensive toxicity and inhibited the normal development of Culex quinquefasciatus mosquitoes by augmented production of reactive oxygen species, indicating its prominent role in oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Rajan

2021

JCD

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“…Taking this into account, it is necessary to use complementary tools that allow a correct identification of individuals. Among these tools it is recommended to use molecular techniques such as DNA barcoding (Cook et al, 2005;Anoopkumar et al, 2019). In this study was described the precision of taxonomic keys and DNA barcoding to classify individuals of the genus Aedes.…”

Section: Discussionmentioning

confidence: 99%

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

et al. 2020

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The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus Aedes and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with Aedes albopictus initially determined as Aedes aegypti based on morphology (n = 13). Molecular identification showed the simultaneous presence of A. aegypti (n = 111) and A. albopictus (n = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for A. aegypti and 39.7% (23/58) for A. albopictus, which was followed by CHIKV with an infection rate of 1.8% for A. aegypti (2/111) and 6.9% for A. albopictus (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of A. aegypti (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying Aedes species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.

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“…They also transmit parasites and protozoa such as Dirofilaria spp. and Plasmodium spp., respectively, and perhaps even bacteria, Rickettsia felis [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Despite decades of extensive research, there are considerable gaps in our current knowledge of the taxonomy of mosquitoes [7,8]. This is of concern, as accurate identification of mosquito species is crucial for the development of optimal control programs and limiting the risk of mosquito-borne diseases [4]. This is becoming more important with expanding urbanization, agriculture, and tourism leading to increased numbers of people invading the natural habitats and breeding sites of mosquitoes [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes

et al. 2022

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Background Accurate identification of mosquito species is essential for the development and optimization of strategies to control mosquitoes and mosquito-borne diseases. Problems with the morphological identification of mosquito species have led to the use of molecular identification techniques, in particular the Folmer cytochrome c oxidase subunit I (COI) PCR system (FCOS), originally designed to identify a range of other invertebrates. Methods As there can be difficulties identifying mosquitoes using FCOS, we re-evaluated the FCOS primers and developed a new COI-based SYBR PCR (the Auburn COI system—AUCOS) to improve the molecular identification of mosquitoes. Sequence data in GenBank for 33 species from 10 genera of mosquitoes were used to develop our AUCOS primers. Two molecular assays (AUCOS, FCOS) and morphological identification were carried out on mosquitoes collected from the field in Auburn, Alabama (USA) and on Saint Kitts. Results With a convenience sample of individual mosquitoes comprising 19 species from six genera in Saint Kitts (n = 77) and Auburn (n = 48), our AUCOS provided higher-quality sequence data than FCOS. It also proved more sensitive than FCOS, successfully amplifying 67.5% (85/126) as opposed to 16.7% (21/126) of the samples. The species determined by morphology, or genus with damaged samples, matched that as determined by AUCOS for 84.9% (62/73) of the samples. Morphological classification was confirmed by FCOS with 81.0% (17/21) of samples producing utilizable sequences. While both FCOS and AUCOS correctly identified all the Aedes, Anopheles, Deinocerites, and Uranotaenia species in the study, identification of Culex species was less successful with both methods: 50.0% (3/6) by FCOS and 35.7% (5/14) by AUCOS. Conclusions The AUCOS DNA barcoding system for mosquito species described in this study is superior to the existing FCOS for the identification of mosquito species. As AUCOS and FCOS amplify the same variable region of the COI, the large amount of existing data on GenBank can be used to identify mosquito species with sequences produced by either PCR. Graphical Abstract

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Molecular characterization of Aedes, Culex, Anopheles, and Armigeres vector mosquitoes inferred by mitochondrial cytochrome oxidase I gene sequence analysis

Cited by 18 publications

References 40 publications

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Activation of the Oxidative Stress in Culexquinquefasciatus by the Augmented Productionof Reactive Oxygen Species (ROS) in responseto Stachytarpheta jamaicensis Exposure

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes

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