Molecular characterization of Aedes aegypti (L.) (Diptera: Culicidae) of Easter Island based on analysis of the mitochondrial ND4 gene

Núñez, Claudia; González, Christian R.; Obreque, Victor; Riquelme, Brenda; Reyes, Carolina; Rojas, Mabel López

doi:10.1016/j.rbe.2016.03.001

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Consequently, many studies have utilized mtDNA to investigate the population genetics and phylogeography of Ae. aegypti around the world (Bosio et al, 2005;Herrera et al, 2006;Paduan and Ribolla, 2008;Twerdochlib et al, 2012;Núñez et al, 2016;Sousa et al, 2017;Dharmarathne et al, 2021). Among them, mitochondrial cytochrome oxidase subunit 1 (COI) gene, and mitochondrial NADH dehydrogenase subunit 4 (ND4) gene are the most widely used mtDNA markers.…”

Section: Abstract: Aedes Aegypti Mitochondrial Nd4 Multiple Invasions...mentioning

confidence: 99%

Population genetic and phylogeographic relationships of the dengue vector <em>Aedes aegypti</em> inferred from three localities of Sri Lanka

Nirmani

Madushani

Perera

et al. 2022

Sri Lankan J. Biol.

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Aedes aegypti is the major epidemic vector of dengue outbreaks in the world. Here, a 359 bp region of the mitochondrial NADH hydrogenase subunit 4 (ND4) gene was taken as the candidate locus for study purposes from 27 Aedes aegypti mosquito larvae collected from an inland mountain area (Badulla), and two distantly located coastal areas (Hambantota and Trincomalee) in Sri Lanka. Eleven parsimonious informative sites were observed, and 7 haplotypes were identified from the 27 samples. The coastal populations displayed higher genetic diversity compared to those from the inland mountain-contained region. The phylogenetic analyses of Ae. aegypti from Badulla, Hambantota and Trincomalee including published Sri Lankan and global samples (a total of 106 sequences) revealed two separate clades: a basal clade and a derived clade. Eighty percent of the samples found from Badulla, were of the basal haplotype (H1) clustered together with West African (Senegal) samples in close proximity to the outgroup. On the contrary, Hambantota and Trincomalee samples were found dispersed in both the basal and derived clades clustering closely with samples from Mexico, Brazil, Kenya, USA and Myanmar indicating their close genetic relationship to many New World, East African and Asian varieties. Taken together, our study proposes that colonization of Ae aegypti in Sri Lanka occurred through multiple founder events (ancient multiple invasions in coastal sites and a more recent West African invasion to the inland mountain-demarcated Badulla region) and continue to exist in mutational drift equilibrium following the settlement event.

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Section: Abstract: Aedes Aegypti Mitochondrial Nd4 Multiple Invasions...mentioning

confidence: 99%

Population genetic and phylogeographic relationships of the dengue vector <em>Aedes aegypti</em> inferred from three localities of Sri Lanka

Nirmani

Madushani

Perera

et al. 2022

Sri Lankan J. Biol.

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“…The mitochondrial gene ND4, which codifies the subunit 4 of the NADH dehydrogenase enzyme, along with the cytochrome c oxidase subunit 1 -COI, and internal transcribed spacer -ITS2, have been considered efficient molecular markers to study the genetic variability of natural populations of Aedes populations (Al Ali et al, 2016;Khater et al, 2021;Escobar et al, 2022). ND4 has been used in multiple populations from Brazil (Bracco et al, 2007;Paduan and Ribolla, 2008;Lima Júnior and Scarpassa, 2009;Twerdochlib et al, 2012), Mexico (Gorrochotegui-Escalante et al, 2002), Peru (Costa-Da-Silva et al, 2005Yáñez et al, 2013), Venezuela (Herrera et al, 2008;Urdaneta-Marquez et al, 2008), Bolivia (Paupy et al, 2012), Colombia (Caldera et al, 2013;Aguirre-Obando et al, 2015), and Chile (Núñez et al, 2016). Therefore, this study aimed to recognize the PYs resistance considering the frequency of kdr V1016I and F1534C mutations, as well as to understand the genetic variation of the natural populations of A. aegypti from Paraná -BR according to the analysis of ND4 mitochondrial gene that helps improve decisions towards the implementation of vector management and control strategies.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and Kdr mutations of natural Aedes (Stegomyia) aegypti (Diptera: Culicidae) populations of Brazil

Ferreira,

Aguirre-Obando,

Twerdochlib

et al. 2023

Rev. Bras. entomol.

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Aedes (Stegomyia) aegypti is an important vector of dengue, yellow fever, chikungunya and Zika virus. It is well known that resistance monitoring and genetic diversity data help designing the vector control programs. This study aimed to evaluate resistance to pyrethroids (PYs) through the frequency of kdr mutations Val1016IIe and F1534C, and the genetic variation of the mitochondrial gene ND4 in six natural populations of A. aegypti from Paraná -Brazil. Adults were obtained from eggs collected from Alvorada do Sul, Marilena, Maringá, Nova Londrina, Paranavaí and São Carlos do Ivaí. From these adults, 345 were used to identify the 1016 and 1534 sites, and 120 were used to perform the ND4 gene analysis. The studied populations from Paraná showed PYs resistance, low gene flow and genetic diversity. Additionally, a relationship was observed among the haplotypes of populations from the Amazon and Southeastern Brazil, Peru, Mexico, and North America.

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Molecular characterization of Aedes aegypti (L.) (Diptera: Culicidae) of Easter Island based on analysis of the mitochondrial ND4 gene

Cited by 2 publications

References 12 publications

Population genetic and phylogeographic relationships of the dengue vector <em>Aedes aegypti</em> inferred from three localities of Sri Lanka

Population genetic and phylogeographic relationships of the dengue vector <em>Aedes aegypti</em> inferred from three localities of Sri Lanka

Genetic diversity and Kdr mutations of natural Aedes (Stegomyia) aegypti (Diptera: Culicidae) populations of Brazil

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