Molecular characterization of a wheat -Thinopyrum ponticum partial amphiploid and its derived substitution line for resistance to stripe rust

Abstract

Stripe rust (caused by Puccinia striiformis) occurs annually in most wheat-growing areas of the world. Thinopyrum ponticum has provided novel rust resistance genes to protect wheat from this fungal disease. Wheat - Th. ponticum partial amphiploid line 7430 and a substitution line X005 developed from crosses between wheat and 7430 were resistant to stripe rust isolates from China. Genomic in situ hybridization (GISH) analysis using Pseudoroegneria spicata genomic DNA as a probe demonstrated that the partial amphiploid line 7430 contained ten J(s) and six J genome chromosomes, and line X005 had a pair of J(s)-chromosomes. Giemsa-C banding further revealed that both lines 7430 and X005 were absent of wheat chromosomes 6B. The EST based PCR confirmed that the introduced J(s) chromosomes belonging to linkage group 6, indicating that line X005 was a 6J(s)/6B substitution line. Both resistance observation and sequence characterized amplified region (SCAR) markers displayed that the introduced chromosomes 6J(s) were responsible for the stripe rust resistances. Therefore, lines 7430 and X005 can be used as a donor in wheat breeding for stripe rust resistance.

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

“…Further, PCR markers based on conserved genetic regions show high levels of collinearity among the cereal genomes, such as rice, Brachypodium, and wheat [Ishikawa et al, 2007]. We previously used PLUG markers to identify chromosomal introgressions from Thinopyrum intermedium [Hu et al, 2011a], T. ponticum [Hu et al, 2011b], and Secale africanum [Lei et al, 2012] and its subsequent assignment to the corresponding linkage group. In the present study, the wheat chromosome group-2 PLUG markers detected polymorphic fragments specific to the D. breviaristatum chromosome in D11-5 ( fig.…”

Identification of a novel wheat-Thinopyrum ponticumaddition line revealed with cytology, SSR, EST-SSR, EST-STS and PLUG markers

“…ponticum and 1-27, and the result of BLAST also supported that it belongs to the group 7. Similarly, Hu et al (2011) sequenced the amplification of the EST-STS marker MAG2114 on specific bands from wheat-Thinopyrum ponticum substitution line X005, and sequence analysis confirmed that the introduced Th. ponticum chromosomes belonged to homologous group 6.…”

“…ponticum, where there is a lack of molecular biology information, the transfer of wheat molecular markers to the species is a feasible method for genetic analysis (Dobrovolskaya et al 2009). Over the last two decades, SSR markers have been used extensively as a effective tool for the genetic analysis of alien chromosomes in various species, such as Thinopyrum ponticum (Hu et al 2011), Thinopyrum intermedium (Lin et al 2007), Triticum timopheevii (Uhrin et al 2012), Leymus mollis (Zhao et al 2013) and Secale cereale (Tang et al 2008).…”

“…Further development of wheat-Th. ponticum introgression lines diverse parental germplasm sources may possess valuable genes or alleles, which could increase the potential of successful breeding in wheat (Hu et al 2011;Lei et al 2011).…”

Characterization of New Wheat-<b><i>Dasypyrum breviaristatum</i></b> Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

“…In order to locate the breakpoints of the translocation chromosomes, 3AL-specific primers were designed based on the GSP web platform for the target regions. PCR was performed as described by Hu et al [2011b].…”