Molecular characterization of a novel virga-like virus associated with wheat

Abstract

In this work, we report the detection of a novel single strand RNA virus from wheat tentatively named as Triticum aestivum-associated virga-like virus 1 (TaAVLV1). Further characterization revealed that the complete genome of TaAVLV1 can be divided into two segments, RNA1 and RNA2. Each fragment excluding their respective polyA tails has 3530 and 3466 nt in length and contains only one open reading frame (ORF). The ORF in RNA1 encodes an RNA-dependent RNA polymerase (RdRp), while the other in RNA2 encodes a putative protein carrying MET and HEL domains. Phylogenetic analysis showed that the RdRp of TaAVLV1 was closely related to the RdRp protein members of the unclassi ed virga-like virus group in the family Virgaviridae. Therefore, we have identi ed TaAVLV1 as a putative novel virga-like virus belonging to the family Virgaviridae.

Seven new mycoviruses identified from isolated ascomycetous macrofungi

“…According to the 2020 ICTV taxonomy report, Virgaviridae contains seven genera including Furovirus, Hordeivirus, Goravirus, Pecluvirus, Pomovirus, Tobamovirus, and Tobravirus . In recent years, several virga-like viruses have been discovered from transcriptomic or metatranscriptomic sequencing without confirmed hosts, which were recognized as not belonging to any of the above known genera; therefore, they were assigned as unclassified Virgaviridae species ( Thekke-Veetil et al., 2020 ; Quintanilha-Peixoto et al., 2021 ; Chiapello et al., 2020 ; Li et al., 2022a ). In the present phylogenetic tree, these unclassified Virgaviridae species were included in one 54 % supported cluster ( Fig.…”

Discovery of Two Novel Viruses of the Willow-Carrot Aphid, Cavariella aegopodii

“…Meanwhile, Virga viruses generally have three characteristics, including the length of the genome being classified as ssRNA (+), the length being about 6300–13,000 nt, and the genome encoding three ORFs [ 10 , 28 ]. Iflaviruses are a type of insect virus that lack an outer envelope.…”