Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from Penicillium citrinum strain RCEF7060

Huang, Yipeng; Wang, Ping; Wu, Hao; Yang, Cheng; Huang, Bo

doi:10.1007/s00705-022-05671-9

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…The proposed genus Unirnavirus and the proposed family Unirnaviridae were suggested to be established in 2015 [44] and in 2017 [45], respectively. Later, several mycoviruses discovered in different fungal species also supported the establishment of Unirnaviridae, such as FcV1 infecting F. culmorum [43] and Penicillium citrinum non-segmented dsRNA virus 1 infecting Penicillium citrinum [46]. In previous studies, it was reported that the members of the proposed family Unirnaviridae were related to monopartite viruses with a similar genome organization; these belong to the family Amalgaviridae or the proposed genus Ustivirus [42,43,46].…”

Section: Discussionmentioning

confidence: 91%

“…Later, several mycoviruses discovered in different fungal species also supported the establishment of Unirnaviridae, such as FcV1 infecting F. culmorum [43] and Penicillium citrinum non-segmented dsRNA virus 1 infecting Penicillium citrinum [46]. In previous studies, it was reported that the members of the proposed family Unirnaviridae were related to monopartite viruses with a similar genome organization; these belong to the family Amalgaviridae or the proposed genus Ustivirus [42,43,46]. In this study, phylogenetic analysis showed that FoV1-FON formed a well-supported independent clade together with members of the genus Unirnavirus within Unirnaviridae, and presented a distant relation with amalgaviruses or ustiviruses.…”

Section: Discussionmentioning

confidence: 98%

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A Novel Strain of Fusarium oxysporum Virus 1 Isolated from Fusarium oxysporum f. sp. niveum Strain X-GS16 Influences Phenotypes of F. oxysporum Strain HB-TS-YT-1hyg

Hua,

Zhang,

Xia

et al. 2024

JoF

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A novel strain of Fusarium oxysporum virus 1 (FoV1) was identified from the Fusarium oxysporum f. sp. niveum strain X-GS16 and designated as Fusarium oxysporum virus 1-FON (FoV1-FON). The full genome of FoV1-FON is 2902 bp in length and contains two non-overlapping open reading frames (ORFs), ORF1 and ORF2, encoding a protein with an unknown function (containing a typical −1 slippery motif G_GAU_UUU at the 3′-end) and a putative RNA-dependent RNA polymerase (RdRp), respectively. BLASTx search against the National Center for the Biotechnology Information (NCBI) non-redundant database showed that FoV1-FON had the highest identity (97.46%) with FoV1. Phylogenetic analysis further confirmed that FoV1-FON clustered with FoV1 in the proposed genus Unirnavirus. FoV1-FON could vertically transmit via spores. Moreover, FoV1-FON was transmitted horizontally from the F. oxysporum f. sp. niveum strain X-GS16 to the F. oxysporum strain HB-TS-YT-1hyg. This resulted in the acquisition of the F. oxysporum strain HB-TS-YT-1hyg-V carrying FoV1-FON. No significant differences were observed in the sporulation and dry weight of mycelial biomass between HB-TS-YT-1hyg and HB-TS-YT-1hyg-V. FoV1-FON infection significantly increased the mycelial growth of HB-TS-YT-1hyg, but decreased its virulence to potato tubers and sensitivity to difenoconazole, prochloraz, and pydiflumetofen. To our knowledge, this is the first report of hypovirulence and reduced sensitivity to difenoconazole, prochloraz, and pydiflumetofen in F. oxysporum due to FoV1-FON infection.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%

A Novel Strain of Fusarium oxysporum Virus 1 Isolated from Fusarium oxysporum f. sp. niveum Strain X-GS16 Influences Phenotypes of F. oxysporum Strain HB-TS-YT-1hyg

Hua,

Zhang,

Xia

et al. 2024

JoF

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“…Despite the identi cation of mycoviruses in various Penicillium species [13][14][15][16], it wasn't until 2003 that genome-related cases were reported [17]. With advancements in high-throughput sequencing, over 15 mycoviruses associated with Penicillium infections have been identi ed, including chrysovirus, partitivirus, polymycovirus, totivirus, and unclassi ed viruses [18][19][20][21][22]. Notably, ve partitiviruses within the genus Gammapartitivirus have been identi ed in Penicillium, namely Penicillium aurantiogriseum partitivirus 1 (PaPV1), Penicillium brevicompactum virus 1 (PbPV1), Penicillium digitatum gammapartitivirus 1 (PdGV1), PsV-F, and PsV-S [18, 20,23].…”

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confidence: 99%

Complete genome sequence of a novel gammapartitivirus from Penicillium oxalicum RCEF7482

Sun,

Wang,

et al. 2024

Arch Virol

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Penicillium oxalicum, an essential biocontrol fungus in China, has been a subject of extensive study due to its role in combating various pathogenic fungi. Despite the prevalence of mycoviruses with doublestranded (ds) RNA genomes in lamentous fungi, no instances of mycovirus infection have been reported in P. oxalicum. In this investigation, we present the identi cation and characterization of a novel dsRNA virus isolated from P. oxalicum, designated as "Penicillium oxalicum partitivirus 1" (PoPV1). The genome of PoPV1 consists of two dsRNA segments , dsRNA1 (1,770 bp) and dsRNA2 (1,584 bp), each hosting a single open reading frame (ORF): ORF1 and ORF2. Comparative analysis revealed that PoPV1's RdRp and CP sequences share the highest identity (89.18% and 73.97%, respectively) with Penicillium aurantiogriseum partitivirus 1 (PaPV1). Motif analysis based on RdRp amino acid sequences places PoPV1 in the genus Gammapartitivirus within the familyPartitiviridae, with a distinctive motif VI (R/KV/ILGDD). Phylogenetic analysis further establishes PoPV1's close relationship with PaPV1, forming a unique clade within gammapartitiviruses. Consequently, we propose that Penicillium oxalicum partitivirus 1 represents a novel species in the genus Gammapartitivirus, marking the rst reported case of dsRNA in P. oxalicum. Full Text Mycoviruses, also known as fungal viruses, are widely distributed among various fungi, showcasing diverse genome types, including double-stranded (ds) RNA, positive-sense (+) single-stranded (ss) RNA, negative-sense (−) ssRNA, or ssDNA. The International Committee on Taxonomy of Viruses (ICTV) has meticulously classi ed mycoviruses into 30 taxa and two unclassi ed genera [1], highlighting their genomic diversity and signi cance in the fungal kingdom.

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A novel gammapartitivirus from the Penicillium oxalicum RCEF7482

Huang,

Sun,

Wang

et al. 2023

Preprint

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Penicillium oxalicum, an essential biocontrol fungus in China, has been a subject of extensive study due to its role in combating various pathogenic fungi. Despite the prevalence of mycoviruses with double-stranded (ds) RNA genomes in filamentous fungi, no instances of mycovirus infection have been reported in P. oxalicum. In this investigation, we present the identification and characterization of a novel dsRNA virus isolated from P. oxalicum, designated as "Penicillium oxalicum partitivirus 1" (PoPV1). The genome of PoPV1 consists of two dsRNA segments, dsRNA1 (1,770 bp) and dsRNA2 (1,584 bp), each hosting a single open reading frame (ORF): ORF1 and ORF2. Comparative analysis revealed that PoPV1's RdRp and CP sequences share the highest identity (89.18% and 73.97%, respectively) with Penicillium aurantiogriseum partitivirus 1 (PaPV1). Motif analysis based on RdRp amino acid sequences places PoPV1 in the genus Gammapartitivirus within the familyPartitiviridae, with a distinctive motif VI (R/KV/ILGDD). Phylogenetic analysis further establishes PoPV1's close relationship with PaPV1, forming a unique clade within gammapartitiviruses. Consequently, we propose that Penicillium oxalicum partitivirus 1 represents a novel species in the genus Gammapartitivirus, marking the first reported case of dsRNA in P. oxalicum.

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Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from Penicillium citrinum strain RCEF7060

Cited by 3 publications

References 18 publications

A Novel Strain of Fusarium oxysporum Virus 1 Isolated from Fusarium oxysporum f. sp. niveum Strain X-GS16 Influences Phenotypes of F. oxysporum Strain HB-TS-YT-1hyg

A Novel Strain of Fusarium oxysporum Virus 1 Isolated from Fusarium oxysporum f. sp. niveum Strain X-GS16 Influences Phenotypes of F. oxysporum Strain HB-TS-YT-1hyg

Complete genome sequence of a novel gammapartitivirus from Penicillium oxalicum RCEF7482

A novel gammapartitivirus from the Penicillium oxalicum RCEF7482

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