Molecular Characterization of a Non–<i>Babesia divergens</i>Organism Causing Zoonotic Babesiosis in Europe

Herwaldt, Barbara L.; Cacciò, Simone M.; Gherlinzoni, Filíppo; Aspöck, Horst; Slemenda, Susan B.; Piccaluga, Pierpaolo; Martinelli, Giovanni; Edelhofer, Renate; Hollenstein, Ursula; Poletti, Giovanni; Pampiglione, S; Löschenberger, Karin; Tura, S; Pieniążek, Norman J.

doi:10.3201/eid0908.020748

Cited by 293 publications

(309 citation statements)

References 25 publications

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“…In contrast, Babesia sp. EU1 which has repeatedly been identified in game or in ticks in Europe (Casati et al, 2006;Duh et al, 2005a;Duh et al, 2005b;Hilpertshauser et al, 2006 and this work) and which also has been reported in three splenectomized human patients (Haselbarth et al, 2007;Herwaldt et al, 2003), differs by 3% in this gene sequence. The analyses of part of the 28S rRNA gene and of the more variable locus, the rDNA ITS, revealed again a high similarity, but the isolates from cattle could clearly be differentiated from those of free-ranging ruminants (Fig.…”

Section: Discussionmentioning

confidence: 87%

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Schmid

Deplazes

Hoby

et al. 2008

Veterinary Parasitology

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Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation Schmid, N Schmid, N. Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation. 2008 Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin -an epidemiological and molecular genetic investigation AbstractIn 2005 and 2006, three adult female chamois (Rupicapra r. rupicapra) were found dead with signs of acute babesial infection in the eastern Swiss Alps. PCR on DNA extracted from blood or spleen of the carcasses revealed sequence identity of the amplified part of the 18S rRNA gene with GenBank entries attributed to Babesia divergens of cattle origin or B. capreoli of wild ruminant origin which have never been described before in this region. Examination of 424 blood samples from 314 head of cattle from this area by IFAT, microscopy and PCR provided no evidence for babesial infection. Six of 887 ticks collected from cattle were PCR-positive, and sequencing revealed Babesia sp. genotype EU1 in five and B. divergens/B. capreoli in one of them. A Babesia isolate of chamois, two isolates of roe deer from the same region and one isolate of a roe deer from the north-western Swiss Alps were genetically compared with two Swiss B. divergens isolates of cattle origin by analysing the genomic rDNA locus. Whereas the near full length sequences of the 18S rRNA gene were virtually identical among all six isolates (>99.4% identity), distinct differences between the two isolates from cattle on the one hand and the four isolates from free-ranging ruminants on the other hand were observed in the sequences of the internal transcribed spacers 1 and 2 (ITS1, ITS2) and part of the 28S rRNA gene. These results indicate that, albeit genetically very closely related, these babesial organisms from cattle and from free-ranging ruminants indeed are distinguishable organisms with different host specificities, and they support the use of the discrete species name B. capreoli for the B. divergens-like organisms from chamois and roe deer. were virtually identical among all six isolates (>99.4% identity), distinct differences between the two isolates from cattle on the one hand and the four isolates from freeranging ruminants on the other hand were observed in the sequences of the internal transcribed spacers 1 and 2 (ITS1, ITS2) and part of the 28S rRNA gene. These results indicate that, albeit genetically very closely related, these babesial organisms from cattle and from free-ranging ruminants indeed are distinguishable organisms with different host specificities, and they support the use of the discrete species name B. capreoli for the B. ...

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Section: Discussionmentioning

confidence: 87%

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Schmid

Deplazes

Hoby

et al. 2008

Veterinary Parasitology

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“…In Europe, Babesia infections are caused mainly by Babesia divergens (142). The majority of cases described occurred in splenectomized patients (166).…”

Section: Other Parasitic Infectionsmentioning

confidence: 99%

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

et al. 2008

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SUMMARY In recent years, the increasing number of donors from different regions of the world is providing a new challenge for the management and selection of suitable donors. This is a worldwide problem in most countries with transplantation programs, especially due to the increase in immigration and international travel. This paper elaborates recommendations regarding the selection criteria for donors from foreign countries who could potentially transmit tropical or geographically restricted infections to solid-organ transplant recipients. For this purpose, an extensive review of the medical literature focusing on viral, fungal, and parasitic infections that could be transmitted during transplantation from donors who have lived or traveled in countries where these infections are endemic has been performed, with special emphasis on tropical and imported infections. The review also includes cases described in the literature as well as risks of transmission during transplantation, microbiological tests available, and recommendations for each infection. A table listing different infectious agents with their geographic distributions and specific recommendations is included.

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“…Babesia sp. EU1 is closely related to Babesia odocoilei (a parasite of white-tailed deer) and based on a 31-nucleotide difference over the complete 18S rRNA gene sequence has been placed phylogenetically in a sister group with B. divergens (Herwaldt et al 2003). Detection of Babesia sp.…”

Section: Introductionmentioning

confidence: 99%

“…A novel species recently described in Europe is Babesia sp. EU1, proposed as Babesia venatorum (Herwaldt et al 2003). Roe deer are thought to be the natural host of this potentially zoonotic species.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium

Lempereur

Lebrun²,

Cuvelier³

et al. 2011

Parasitol Res

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Anaplasmosis and babesiosis are major tick-borne diseases with a high economic impact but are also a public health concern. Blood samples collected in the spring, summer, and autumn of 2010 from 65 cows in seven different farms in Belgium were monitored with an indirect immunofluorescence antibody test to assess seroprevalence against these pathogens. Seroprevalences to Babesia spp. were measured as 10.7%, 20%, and 12.3% in spring, summer, and autumn, respectively, whereas seroprevalences to Anaplasma phagocytophilum were 30.8%, 77%, and 56.9%, respectively. A total of 805 Ixodes ricinus ticks were collected at the same time from both cattle (feeding ticks) and grazed pastures (questing ticks). The infection level of ticks, assessed by PCR assay, for Babesia spp. DNA was 14.6% and 7.9% in feeding and questing ticks, respectively, whereas 21.7% and 3% of feeding and questing ticks were found be positive for A. phagocytophilum cDNA. Fifty-five PCR-positive samples were identified by sequencing as Babesia sp. EU1, of which five from feeding ticks were positive for both A. phagocytophilum and Babesia sp. EU1. The high density of wild cervids in the study area could explain these observations, as deer are considered to be the main hosts for adults of I. ricinus. However, the absence of Babesia divergens both in feeding and questing ticks is surprising, as the study area is known to be endemic for cattle babesiosis. Increasing cervid populations and comorbidity could play an import role in the epidemiology of these tickborne diseases.

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Molecular Characterization of a Non–Babesia divergensOrganism Causing Zoonotic Babesiosis in Europe

Cited by 293 publications

References 25 publications

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Babesia divergens-like organisms from free-ranging chamois (Rupicapra r. rupicapra) and roe deer (Capreolus c. capreolus) are distinct from B. divergens of cattle origin – An epidemiological and molecular genetic investigation

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

Longitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium

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