Molecular characterization of a new whitefly-transmissible bipartite geminivirus infecting tomato in Panama.

Engel, Margit; Fernández, Orencio; Jeske, Holger; Frischmuth, Thomas

doi:10.1099/0022-1317-79-10-2313

Cited by 30 publications

(31 citation statements)

References 25 publications

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“…, Tomato yellow mosaic virus (ToYMV) was described as an occasional pathogen of potato (S. tuberosum) in the state of Aragua, Venezuela. Unfortunately, ToYMV is the most important neotropical begomovirus of tomatoes in the Caribbean Basin, where it affects tomato production in Guadeloupe, Martinique, Trinidad and Tobago, Puerto Rico and the Dominican Republic in the Antilles Urbino et al 2004), Panama (Engel et al 1998), Colombia (Morales 2006a), Venezuela (Nava et al 2006), and other Latin American regions considered part of the Caribbean Basin. Unfortunately, ToYMV is the most important neotropical begomovirus of tomatoes in the Caribbean Basin, where it affects tomato production in Guadeloupe, Martinique, Trinidad and Tobago, Puerto Rico and the Dominican Republic in the Antilles Urbino et al 2004), Panama (Engel et al 1998), Colombia (Morales 2006a), Venezuela (Nava et al 2006), and other Latin American regions considered part of the Caribbean Basin.…”

Section: South Americamentioning

confidence: 99%

“…The first begomovirus affecting tomato production in this region was described in 1998 (Engel et al 1998) as Tomato leaf curl virus, but was later recognized as a strain of ToYMV. The first begomovirus affecting tomato production in this region was described in 1998 (Engel et al 1998) as Tomato leaf curl virus, but was later recognized as a strain of ToYMV.…”

Section: Central Americamentioning

confidence: 99%

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Bemisia: Bionomics and Management of a Global Pest

Brown

2010

143

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No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Cover illustration: Whitefly -USDA-ARS, Scott BauerPrinted on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) This book is dedicated to Dr. Jacquelyn (Jackie) L. Blackmer , a coauthor of Chapter 5 and a renowned entomologist in the field of insect behavior, insect-plant interactions, and insect dispersal and migration. During postdoctoral work at The University of Arizona and her most recent tenure with the United States Department of Agriculture, Agricultural Research Service in Arizona she made numerous advances in understanding the behavior, physiology and ecology of Bemisia tabaci. Dr. Blackmer conducted some of the first and still the most comprehensive studies on the migratory and short-range flight behavior of Bemisia tabaci including the role of environment, physiology, morphology, life history and host quality. She advanced our understanding of host quality factors in whitefly life history, developed an artificial diet system, and examined the flight behavior of whitefly parasitoids. She was a tireless, innovative and inspirational researcher as well as being a generous collaborator, colleague and friend to all with whom she worked. Her significant contributions to entomology will be long remembered and will continue to inspire advances in whitefly biology, ecology and management.v

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Section: South Americamentioning

confidence: 99%

Section: Central Americamentioning

confidence: 99%

Bemisia: Bionomics and Management of a Global Pest

Brown

2010

143

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“…), Potato yellow mosaic Panama virus (PYMPV) (Engel et al. ; Valderrama et al. ), Tomato golden mottle virus (ToGMoV) (Maxwell et al.…”

Section: Introductionmentioning

confidence: 99%

Begomoviruses Infecting Tomato Crops in Panama

Herrera-Vásquez¹,

Ortega

Romero³

et al. 2015

Journal of Phytopathology

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The key regions in Panama involved in open field- and greenhouse-grown commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santos, Coclé and Panama Oeste provinces, were surveyed for the incidence and distribution of begomoviruses in the growing seasons of 2011 and 2012. The surveys took place in 14 of the 51 districts of the above-mentioned provinces and comprised all relevant tomato production areas of the provinces. A total of 28 tomato plots were surveyed. The exact location of each plot was geo-referenced using a hand-held Global Positioning System unit. In total, 319 individual tomato plants (181 in 2011 and 138 in 2012) were sampled. Plants displayed diverse combinations of virus-like symptoms of different severity, including necrosis, yellowing, mosaic, mottling, rolling, curling, distortion and puckering of leaves, reduced leaf size, and stunted growth. DNA was extracted from each plant for a subsequent polymerase chain reaction (PCR) analysis, using two sets of degenerate primers able to detect members of the genus Begomovirus. The samples displaying a positive reaction were subsequently analysed with specific primer pairs to identify the affecting begomoviruses. A total of 42.3% of all collected samples showed a positive signal to PCRs. Three begomovirus species were detected with the species-specific set of primers; in particular, in the samples obtained in 2011, Potato yellow mosaic Panama virus (PYMPV), Tomato leaf curl Sinaloa virus (ToLCSiV) and Tomato yellow mottle virus (TYMoV) were detected, while in the 2012 samples, only PYMPV and ToLCSiV were found. To our knowledge, this is the first reported incidence of ToLCSiV and TYMoV in Panamanian tomato crops

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“…BLAST analysis of two PCR products' sequences of the distinct viruses (GenBank Accession Nos. KP313717 for PYMPV and KP318651 for ToLCSiV, respectively) revealed that KP313717 shared 99 % DNA sequence identity with PYMPV -[Panama:Divisa:Tomato:1996] (PYMPV-[PA:Div:Tom:96], (Y15034) (Engel et al, 1998), and KP318651 99 % identity with ToLCSiV -[Nicaragua:Santa Lucia] (ToLCSiV-[NI:SL], (AJ608286) (Rojas et al, 2005) and three other ToLCSiV sequences: [Nicaragua:Santa Lucia] (AJ508779), [Nicaragua:Sebaco] (AJ508780) (Rojas et al, 2005), and [Costa Rica:Alajuela] (AF131213); as well as 98 % identity with ToLCSiV-[Nicaragua:Condega] (AJ508778) (Rojas et al, 2005).…”

Section: This Analysis Revealed Potato Yellow Mosaic Panama Virus (Pymentioning

confidence: 99%

“…This corresponds to an incidence of 26% (8 plants) in open field, and 43% (41 plants) in greenhouse crops. Primers specific for ten tomato-infecting begomoviruses found in Central America (Engel et al, 1998;Nakhla et al, 2005; Table 1) were used to typify the PCR-positive samples.…”

Section: Figurementioning

confidence: 99%