Molecular characterization of a genetically divergent equine pegivirus strain identified in China

Lu, Gang; Fu, Cheng; Huang, Ji; Xu, Haibin; Wu, Peixin; Ping, Xiaokun; Li, Shoujun

doi:10.1007/s00705-017-3602-z

Cited by 6 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For each sample, 8 µL of viral RNA was subsequently used for cDNA synthesis. EqHV, EPgV, and TDAV RNA were detected using the cDNA as a PCR template according to the experimental procedure described in our previous studies 15 , 23 , 24 . EqPV-H DNA was detected by nested PCR using Genestar taq polymerase premix (Kangrun, China) according to the manufacturer’s instructions with the PCR primers reported by Thomas J.…”

Section: Methodsmentioning

confidence: 99%

Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China

Sun

et al. 2018

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

A novel equine parvovirus, equine parvovirus-hepatitis (EqPV-H), was first discovered in a horse that died of equine serum hepatitis in the USA in 2018. EqPV-H was shown to be a novel etiological agent associated with equine serum hepatitis. Following this initial report, no additional studies on EqPV-H have been published. In this study, a total of 143 serum samples were collected from racehorses at 5 separate farms in China and were analyzed to detect EqPV-H DNA via nested PCR. The results indicated a high prevalence of EqPV-H (11.9%, 17/143) in the studied animals. In addition, a remarkably high coinfection rate (58.8%, 10/17) with 2 equine flaviviruses (equine hepacivirus and equine pegivirus) was observed in the EqPV-H positive equines. However, all equines tested negative for Theiler’s disease-associated virus, an etiological agent associated with equine serum hepatitis. The genomes of six field EqPV-H strains were sequenced and analyzed, with the results indicating that the Chinese EqPV-H strains have low genetic diversity and high genetic similarity with the USA EqPV-H strain BCT-01. A phylogenetic analysis demonstrated that the Chinese EqPV-H strains clustered with BCT-01 in the genus Copiparvovirus but were distantly related to another equine parvovirus identified in horse cerebrospinal fluid. In addition, liver enzyme levels were detected in the EqPV-H positive serum samples, and all the values were in the normal range, indicating that infection can occur without concurrent liver disease. This study will promote an understanding of the geographical distribution, genetic diversity, and pathogenicity of EqPV-H.

show abstract

Section: Methodsmentioning

confidence: 99%

Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China

Sun

et al. 2018

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

show abstract

“…TDAV is closer genetically to the GB virus than are HCV and NPHV. By detecting viral RNA and/or antibody against viral proteins in serum or plasma, EqHV and EPgV have been identified in the equine population in American, European and Asian countries[ 10 , 12 – 18 ]. However, equines infected with TDAV have not been identified in other studies worldwide since the first reports[ 12 , 13 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and genetic characterization of hepacivirus and pegivirus in commercial equine serum products in China

Huang

Yang

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Equine hepacivirus (EqHV), equine pegivirus (EPgV) and Theiler’s disease-associated virus (TDAV) are three novel equine viruses in the family Flaviviridae. EqHV and EPgV have been identified to circulate in the equine population worldwide, whereas TDAV has not been detected in equines since the first reported case. To date, no studies have focused on investigating EqHV, EPgV and TDAV in commercial equine sera or equine blood products in China. Considering the potential threat of EqHV, EPgV and TDAV to biosecurity and considering their possible influences on research results, equine sera for cell culture propagation and pregnant mare serum gonadotropin (PMSG) were purchased from different companies in China and investigated for EqHV, EPgV and TDAV in this study. By performing nested PCR or two rounds of PCR targeting the viral NS3 gene, four serum samples were confirmed to be EqHV-, EPgV-, or TDAV-RNA positive; all of the PMSG samples were negative for these three viruses. Subsequent sequencing results indicated that the serum samples contained multiple viral variants of EqHV, EPgV or TDAV, and a genetic analysis based on the partial NS3 gene of the three equine viruses was performed. Our study is the first to confirm the presence of EqHV, EPgV and TDAV in equine sera for cell culture propagation that is commercially available in China and provides the first demonstration of the presence of TDAV in China.

show abstract

Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco

Abbadi

Lkhider²,

Kitab

et al. 2021

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Molecular characterization of a genetically divergent equine pegivirus strain identified in China

Cited by 6 publications

References 10 publications

Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China

Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China

Identification and genetic characterization of hepacivirus and pegivirus in commercial equine serum products in China

Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco

Contact Info

Product

Resources

About