Molecular characterization of a distinct monopartite begomovirus associated with betasatellites and alphasatellites infecting Pisum sativum in Nepal

Shahid, Muhammad; Pudashini, B. J.; Khatri-Chhetri, G. B.; Briddon, R. W.; Natsuaki, Keiko T.

doi:10.1007/s11262-016-1424-9

Cited by 8 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total nucleic acid was extracted from leaf samples using a cetyltrimethylammonium bromide-based method [12] and kept at − 20 °C. Extracted DNA was used as a template in polymerase chain reaction (PCR) with primer pairs for the detection of begomoviruses (TYLCD-356 (5′-ATCATTTCCACKCCCGYCTCGA-3′/TYLCD-1044 5′-GCRTGMGTACABGCCATATACA-3′), amplifying an ~ 800 nt product, betasatellites (Sat101/Sat102), amplifying an ~ 1350 nt product [13], and alphasatellites (DNA101/DNA102), amplifying an ~ 1380 nt product [14]. Additionally the primer pair βC1F (5′-AGACCCGGGATGACGATCAGATATAATAACA-3′)/βC1R (5′-ACGTCGACTCACACACACACTTTCGTACA-3′), amplifying a ~ 350 nt product, was used in PCR for the detection of betasatellites.…”

Section: Methodsmentioning

confidence: 99%

Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman

Shahid

Shafiq

Raza

et al. 2019

Virol J

View full text Add to dashboard Cite

BackgroundIn Oman tobacco (Nicotiana tabacum; family Solanaceae) is a minor crop, which is produced only for local consumption. In 2015, tobacco plants exhibiting severe downward leaf curling, leaf thickening, vein swelling, yellowing and stunting were identified in fields of tobacco in Suhar Al-Batina region, Oman. These symptoms are suggestive of begomovirus (genus Begomovirus, family Geminiviridae) infection.MethodsCircular DNA molecules were amplified from total DNA extracted from tobacco plants by rolling circle amplification (RCA). Viral genomes were cloned from RCA products by restriction digestion and betasatellites were cloned by PCR amplification from RCA product, using universal primers. The sequences of full-length clones were obtained by Sanger sequencing and primer walking. Constructs for the infectivity of virus and betasatellite were produced and introduced into plants by Agrobacterium-mediated inoculation.ResultsThe full-length sequences of 3 begomovirus and 3 betasatellite clones, isolated from 3 plants, were obtained. Analysis of the full-length sequences determined showed the virus to be a variant of Chilli leaf curl virus (ChiLCV) and the betasatellite to be a variant of Tomato leaf curl betasatellite (ToLCB). Both the virus and the betasatellite isolated from tobacco show the greatest levels of sequence identity to isolates of ChiLCV and ToLCB identified in other hosts in Oman. Additionally clones of ChiLCV and ToLCB were shown, by Agrobacterium-mediated inoculation, to be infectious to 3 Nicotiana species, including N. tabacum. In N. benthamiana the betasatellite was shown to change the upward leaf rolling symptoms to a severe downward leaf curl, as is typical for many monopartite begomoviruses with betasatellites.ConclusionsThe leaf curl disease of tobacco in Oman was shown to be caused by ChiLCV and ToLCB. This is the first identification of ChiLCV with ToLCB infecting tobacco. The study shows that, despite the low diversity of begomoviruses and betasatellites in Oman, the extant viruses/betasatellites are able to fill the niches that present themselves.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman

Shahid

Shafiq

Raza

et al. 2019

Virol J

View full text Add to dashboard Cite

show abstract

“…Electron microscopic examination of leaf extracts from leaves showing symptoms narrow and/or curled, and stunting of pea plants at Rampur revealed pea seed-borne mosaic potyvirus (PSbMV) (Dahal and Albrechtsen, 1996). Pea leaf distortion virus (PLDV) was also reported from Rampur, Nepal (Shahid et al, 2017). Dahal and Albrechtsen, (1996) recorded symptoms of cowpea aphid-borne mosaic potyvirus (CABMV) during 1989-1990 at Rampur which was transmitted by both aphids and sap inoculations.…”

Section: Peamentioning

confidence: 99%

Viral Diseases of Crops in Nepal

Poudel

Khanal

2018

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

Viral diseases are the important diseases next to the fungal and bacterial in Nepal. The increase in incidence and severity of viral diseases and emergence of new viral diseases causes the significant yield losses of different crops in Nepal. But the research and studies on plant viral diseases are limited. Most of the studies were focused in viral diseases of rice (Rice tungro virus and Rice dwarf virus), tomato (Yellow leaf curl virus) and potato (PVX and PVY). Maize leaf fleck virus and mosaic caused by Maize mosaic virus were recorded as minor disease of maize. Citrus Tristeza Virus is an important virus of citrus fruit in Nepal while Papaya ringspot potyvirus, Ageratum yellow vein virus (AYVV), Tomato leaf curlJava betasatellite and Sida yellow vein Chinaalphasatellite were recorded from the papaya fruit. The Cucumber mosaic virus (CMV) and Zucchini yellow mosaic potyvirus (ZYMV) are the viral diseases of cucurbitaceous crop reported in Nepal. Mungbean yellow mosaic India virus (MYMIV) found to infect the many crops Limabean, Kidney bean, blackgram and Mungbean. Bean common mosaic necrosis virus in sweet bean, Pea leaf distortion virus (PLDV), Cowpea aphid‐borne mosaic potyvirus (CABMV), Peanut bud necrosis virus (PBNV) in groundnut, Cucumber mosaic virus (CMV). Chili veinal mottle potyvirus (CVMV) and Tomatoyellow leaf curl gemini virus (TYLCV) were only reported and no any further works have been carried out. The 3 virus diseases Soyabean mosaic (SMV), Soybean yellow mosaic virus and Bud blight tobacco ring spot virus (TRSV) were found in soybean.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 75-80

show abstract

“…Information on the occurrence, distribution, and economic loss of begomovirus in Nepal is limited. Until now, only a few begomoviruses, such as Mungbean yellow vein mosaic Indian virus (MYMIV) [26], Pea leaf distortion virus (PLDV) [27], Ageratum enation virus (AEV) [28] and Ageratum yellow vein virus (AYVV) [29] have been reported in Nepal. Ghimire et al [30] reported the occurrence of Tomato yellow leaf curl virus (TYLCV) disease in most tomato growing pockets in Nepal and recorded more than 40% yield loss.…”

Section: Introductionmentioning

confidence: 99%

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

et al. 2021

View full text Add to dashboard Cite

The Bemisia tabaci species complex consists of at least 44 cryptic species, which are potential vectors of approximately 320 begomovirus species, most of which are significant plant viruses. However, the relationship of begomovirus transmission through vectors at the cryptic species level is uncertain. In our previous study, three cryptic species (Asia I, Asia II 1, and Asia II 5) of B. tabaci were identified from 76 B. tabaci samples collected across 23 districts in Nepal. Using the same individuals we identified seven different begomovirus species (Squash leaf curl China virus [SLCCNV], Tomato leaf curl New Delhi virus [ToLCNDV], Okra enation leaf curl virus [OELCuV], Synedrella leaf curl virus [SyLCV], Tomato leaf curl Kerala virus [ToLCKeV], Ageratum enation virus [AEV], and Tomato leaf curl Karnataka virus [ToLCKV]) by PCR using universal begomovirus primers. The begomoviruses were detected in 55.26% of whitefly samples, and SLCCNV was the most prevalent species (27.63%). Among the three cryptic species of B. tabaci, the virus detection rate was highest in Asia I (60%), followed by Asia II 1 (58.82%) and Asia II 5 (53.06%). Most viruses were detected in all three species, but AEV and ToLCKV were found only in Asia I and Asia II 1, respectively. Geographic analysis showed that SLCCNV was distributed in the whole country, which is similar to the distribution of the Asia II 5 species, but OELCuV and SyLCV were detected only in the middle region of Nepal. Our results provide important information on the begomovirus profile in Nepal which can be beneficial for plant virus risk assessment and develop the management strategies to reduce the damage of whitefly transmitted viruses.

show abstract

Molecular characterization of a distinct monopartite begomovirus associated with betasatellites and alphasatellites infecting Pisum sativum in Nepal

Cited by 8 publications

References 32 publications

Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman

Molecular and biological characterization of Chilli leaf curl virus and associated Tomato leaf curl betasatellite infecting tobacco in Oman

Viral Diseases of Crops in Nepal

Identification of Begomoviruses from Three Cryptic Species of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Nepal

Contact Info

Product

Resources

About