Molecular characterization of a cytosolic ascorbate peroxidase in strawberry fruit

Kim, In-Jung; Chung, Won Sang

doi:10.1016/s0168-9452(98)00029-6

Cited by 31 publications

(15 citation statements)

References 28 publications

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“…We have also isolated a partial cDNA encoding a cytosolic APX. At present, there are many reports about cytosolic APX in different plants and fruits (Mittler and Zilinskas, 1991;Ishikawa et al, 1995;Kim and Chung, 1998), but there are no reports on table grapes related to APX gene. In the present study, the analysis of APX gene expression revealed that transcript levels increased slightly both in treated and non-treated grapes, but a sharp increase was observed after 28 days in non-treated grapes where total decay was evident .…”

Section: Article In Pressmentioning

confidence: 99%

“…Ascorbate peroxidases (APX) (EC 1.11.1.11) utilize ascorbic acid and its specific electron to reduce H 2 O 2 to water. APX genes have been isolated from some plants and their expression has been determined under fruit ripening, oxidative stress and low temperature, respectively (Kim and Chung, 1998;Yoshimura et al, 2000;Kawakami et al, 2002). In grape, there have been no reports on the involvement of APX in Botritys attack or on the effect of a beneficial high CO 2 pretreatment in controlling decay; we therefore isolated an APX partial cDNA and analyzed the changes in gene expression in treated and nontreated table grapes stored at low temperatures.…”

Section: Introductionmentioning

confidence: 99%

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Anthocyanin, antioxidant activity and stress-induced gene expression in high CO2-treated table grapes stored at low temperature

Romero¹,

Sanchez-Ballesta²,

Maldonado³

et al. 2008

Journal of Plant Physiology

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Section: Article In Pressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anthocyanin, antioxidant activity and stress-induced gene expression in high CO2-treated table grapes stored at low temperature

Romero¹,

Sanchez-Ballesta²,

Maldonado³

et al. 2008

Journal of Plant Physiology

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“…APX activity was up-regulated substantially when plants were subjected to environmental stresses, for instance, heavy metals, high salinity, drought, high temperature, and wounding (Shi et al, 2001). APX has been cloned from many plant species such as pea (Mittler and Zilinskas, 1991), Arabidopsis (Kubo et al, 1992;Santos et al, 1996;Maruta et al, 2012), maize (van Breusegem et al, 1995), spinach (Webb and Allen, 1995;Yoshimura et al, 1999), tobacco (Orvar and Ellis, 1995), strawberry (Kim and Chung, 1998), barley (Shi et al, 2001), potato (Kawakami et al, 2002), rice (Lu et al, 2005), Vitis pseudoreticulata (Lin et al, 2006), birch (Wang et al, 2009), muskmelon (Cheng et al, 2009), lily (Chen et al, 2010), grain sorghum (Chen et al, 2011), and Nelumbo nucifera (Dong et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum

Liu

Tian

Liu

et al. 2013

J. Zhejiang Univ. Sci. B

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An 888-bp full-length ascorbate peroxidase (APX) complementary DNA (cDNA) gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame (ORF). This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction (RT-PCR) analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage (EL) and malondialdehyde (MDA) content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cell membrane damage and ultimately enhance the plant cold tolerance.

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“…The different modes of regulation and functions of APX isoenyzmes are not well understood. Therefore, most information on cDNAs and genes encoding plant APX has been obtained for pea (Mittler and Zilinskas, 1991), Arabidopsis (Kubo et al, 1992;Santos et al, 1996), spinach (Ishikawa et al, 1996), pumpkin (Yamaguchi et al, 1996), maize (Breusegem et al, 1995), rice (Morita et al, 1997), and strawberry (Kim and Chung, 1998).…”

Section: Southern Blot Analysismentioning

confidence: 99%

“…Studies on APX gene expression in bell pepper, a climacteric fruit, suggested that its expression is related to fruit ripening Putative Ascorbate Peroxidase from Citrus 13 (Schantz et al, 1995). Also, in strawberry, a climacteric fruit, an increased expression of cAPX gene was observed during fruit ripening (Kim and Chung, 1998). All citrus fruit are non climacteric, therefore, studies on relationship between fruit ripening and APX gene expression may provide better insight into the complex physiological changes involved during the process of fruit ripening.…”

Section: Southern Blot Analysismentioning

confidence: 99%

Isolation and Molecular Characterization of a Putative Ascorbate Peroxidase Gene from Citrus

Kunta

Rio

Skaria

et al. 2010

International Journal of Fruit Science

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Ascorbate peroxidase is the key enzyme in detoxifying hydrogen peroxide (H 2 O 2 ) and other reactive oxygen species from chloroplast and cytosol. A cDNA encoding a putative citrus ascorbate peroxidase, APXcit was isolated from mature 'Dancy' tangerine (Citrus reticulata Blanco) juice vesicles using differential display reverse transcription-polymerase chain reaction. The full-length APXcit sequence was composed of 1,082 bp nucleotides, including an open reading frame of 753 bp, putatively encoding a protein of 250 amino acids with a predicted molecular mass of 27 kDa. The 5¢ untranslated region consisted of 90 nucleotides and the 3¢ untranslated region of 239. The genomic clone of 2,457 bp was composed of 8 introns and 9 exons. A homology search for APXcit at the GenBank database showed high similarity to ascorbate peroxidase from several plant species. Southern analysis revealed that there may be 2-4 APXcit gene copies in citrus.

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Molecular characterization of a cytosolic ascorbate peroxidase in strawberry fruit

Cited by 31 publications

References 28 publications

Anthocyanin, antioxidant activity and stress-induced gene expression in high CO2-treated table grapes stored at low temperature

Anthocyanin, antioxidant activity and stress-induced gene expression in high CO2-treated table grapes stored at low temperature

Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum

Isolation and Molecular Characterization of a Putative Ascorbate Peroxidase Gene from Citrus

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