Molecular characterization of a Cyrtochilum loxense Somatic Embryogenesis Receptor-like Kinase (SERK) gene expressed during somatic embryogenesis

Cueva, Augusta; Concia, Lorenzo; Cella, Rino

doi:10.1007/s00299-012-1236-x

Cited by 29 publications

(18 citation statements)

References 56 publications

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“…Three of these, PhSERK1, 2, and 5, showed typical SERK protein structural features, as reported for other plant species. The high sequence similarity of PhSERK1 and 2 with CnSERK (Pérez-Núñez et al, 2009), AcSERK1 and 2 (Ma et al, 2012a,b), ClSERK (Cueva et al, 2012), OsSERK (Ito et al, 2005), MtSERK1 (Nolan et al, 2003), ZmSERK1 (Baudino et al, 2001), and AtSERK1 suggests that PhSERK1 and 2 are involved in PLB formation or somatic embryogenesis, as has been proposed for orthologs from other species. Clustering of PhSERK5 with PpSERK1 and 2 ( Figure 3) also points to a similar function in embryogenesis (Albrecht et al, 2005).…”

Section: Discussionmentioning

confidence: 85%

“…OsSERK1 is thought to be partially responsible for mediating defense signal transduction and has a basic role in rice somatic embryogenesis (Hu et al, 2005). More homologs of SERK have been identified in several plant species, including Dactylis glomerata (Somleva et al, 2000), Medicago truncatula (Nolan et al, 2003(Nolan et al, , 2009, Ocotea catharinensis (Santa-Catarina et al, 2004), Helianthus annuus (Thomas et al, 2004), Theobroma cacao (Santos et al, 2005), Citrus unshiu (Shimada et al, 2005), Triticum aestivum (Singla et al, 2008), Vitis vinifera (Schellenbaum et al, 2008), Solanum tuberosum (Sharma et al, 2008), Cocos nucifera (Perez-Núñez et al, 2009), Lactuca sativa (Santos et al, 2009), Rosa hybrida (Zakizadeh et al, 2010), Musa acuminata (Huang et al, 2010), Secale cereale (Gruszczyńska and Rakoczy-Trojanowska, 2011), Glycine max (Yang et al, 2011), Cyclamen persicum (Savona et al, 2012), Ananas comosus (Ma et al, 2012a,b), and Cyrtochilum loxense (Cueva et al, 2012). These reports all point to SERK as a specific marker of embryogenic competence during morphogenesis induction.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Yw¹,

Yj²,

Fc³

2014

Genet. Mol. Res.

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ABSTRACT. Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich repeat-receptor-like kinase domain. All 5 PhSERKs were expressed in all Phalaenopsis organs examined (root, leaf, shoot apical meristem, and flower), with the strongest expression, particularly for PhSERK1 and 3, in the shoot apical meristem of mature plants. Expression of all PhSERKs was downregulated during early floral bud development and was upregulated gradually until the semiopen flower stage was reached. All 5 PhSERKs were expressed during both seed germination and protocorm-like-body (PLB) development. In germinated seeds, quantitative real-time PCR revealed upregulation of all PhSERKs except PhSERK4 at 1 week and downregulation after 4 weeks. The 5 PhSERKs were differentially expressed in the early stage Characterization of genes from Phalaenopsis of PLB development and maintained substantial levels during PLB formation, with PhSERK1 and 5 upregulated 1 week after culture and PhSERK2, 3, and 4 downregulated over this period. Because physical wounding of PLB stimulates secondary PLB formation, the PhSERK5 expression peak at week 3 coincided with visible and fully developed secondary PLBs. PhSERK5 may be important in PLB induction and subsequent development. Our PhSERK expression analysis revealed that these genes have a broad role during orchid plant development.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Yw¹,

Yj²,

Fc³

2014

Genet. Mol. Res.

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“…In several species, SERK expression has been linked to acquisition of embryogenic competence Thomas et al, 2004;Pérez-Núñez et al, 2009;Cueva et al, 2012;Talapatra et al, 2014). Monitoring of expression during somatic embryogenesis in in vitro cultures revealed that CaSERK was most strongly expressed in embryogenic callus.…”

Section: Discussionmentioning

confidence: 99%

“…The involvement of SERK proteins in regulating somatic embryogenesis was first described for DcSERK in embryogenic cell suspension cultures of carrot more than 15 years ago (Schmidt et al, 1997). Since then SERK gene homologs have been identified and their role in mediating somatic embryogenesis demonstrated in several monocot and dicot species such as Arabidopsis thaliana , Medicago truncatula (Nolan et al, 2003), Triticum aestivum (Singla et al, 2008), Vitis vinifera (Maillot et al, 2009), Cocos nucifera (Pérez-Núñez et al, 2009), Ananas comosus (Ma et al, 2012) and Cyrtochilum loxense (Cueva et al, 2012). Moreover, a gymnosperm SERK homolog has recently been discovered and its association with somatic embryogenesis was shown (Steiner et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul¹,

Rakmit²,

Boonsorn³

et al. 2014

JAS

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Somatic embryogenesis provides a useful tool to facilitate efficient mass propagation in plants. The SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene serves a fundamentally important role in somatic embryogenesis of many plant species. The isolation of a SERK gene homolog, namely CaSERK, from Curcuma alismatifolia Gagnep. cv. Blue Tung, was reported. Prediction of coding sequence showed that it encoded a protein of 628 amino acids showing high similarity to previously characterized SERK sequences and containing all the features shared by members of the SERK family, including five leucine-rich repeats and the distinctive proline-rich SPP domain. Investigation of CaSERK expression revealed that its transcripts were found throughout the whole somatic embryogenesis process with highest abundance in embryogenic callus. These results indicate that CaSERK might have somatic embryogenesis-associated functions in this economically important ornamental ginger. Detection of CaSERK transcript accumulation in flower and coma bract tissues is suggestive of its additional roles in other developmental signaling pathways.

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“…LRR-RLKs are divided into 13 subclasses according to the structure and quantity of different extracellular LRRs (Gou et al, 2010) 2010, and SERK belongs to the second subclass (Shiu and Bleecker, 2001). The protein structure of SERK begins with a signal N-terminal peptide followed by one leu-zipper domain, five LRRs, one ser-pro-ro (SPP) motif, one single transmembrane (TM) domain, three conserved serine/threonine kinase subdomains, and one C-terminal leucine-rich domain (Cueva et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

et al. 2015

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Cloning and characterization of theABSTRACT. This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The fulllength sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid sequences were conducted to identify similarity between MhSERK1 and MhdSERK1 and SERK sequences in other species, and a neighbor-joining phylogenetic tree was constructed to elucidate their phylogenetic relations. Expression levels of MhSERK1 and MhdSERK1 in different tissues and developmental stages were investigated using quantitative real-time PCR. The coding sequence lengths of MhSERK1 and MhdSERK1 were 1899 bp (encoding 632 amino acids) and 1881 bp (encoding 626 amino acids), respectively. Sequence analysis demonstrated that MhSERK1 and MhdSERK1 display high similarity to SERKs in other species, with a conserved intron/exon structure that is unique to members of the SERK 14577 Roles of SERK1 in Pingyi Tiancha and a hybrid strain ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14576-14586 (2015) family. Additionally, the phylogenetic tree showed that MhSERK1 and MhdSERK1 clustered with orange CitSERK (93%). Furthermore, MhSERK1 and MhdSERK1 were mainly expressed in the reproductive organs, in particular the ovary. Their expression levels were highest in young flowers and they differed among different tissues and organs. Our results suggest that MhSERK1 and MhdSERK1 are related to plant reproduction, and that MhSERK1 is related to apomixis in triploid Pingyi Tiancha.

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Molecular characterization of a Cyrtochilum loxense Somatic Embryogenesis Receptor-like Kinase (SERK) gene expressed during somatic embryogenesis

Cited by 29 publications

References 56 publications

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

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