Molecular characterization of a cold-induced plasma membrane protein gene from wheat

Imai, Ryozo; Koike, Michiya; Sutoh, Keita; Kawakami, Akira; Torada, Atsushi; Oono, Kiyoharu

doi:10.1007/s00438-006-0096-x

Cited by 5 publications

(9 citation statements)

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“…Induction of disease resistance-related plasma membrane proteins by plant hormones has not been reported. Cold-regulated plasma membrane protein genes are induced in wheat and rice by ABA treatment (Breton et al 2003;Imai et al 2005;Morsy et al 2005). Inducible CaPIMP1 may be eYcient at mediating and enhancing plant defense responses against abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…For example, two transmembrane domains have been found in Arabidopsis NDR1 (Century et al 1997), and the barley Mlo protein and the cold-regulated wheat COR-413 protein were found to have seven and nine transmembrane domains, respectively (Breton et al 2003;Bhat et al 2005). Only transmembrane helix structures are suYcient to address the biological roles of plasma membrane proteins, including COR-413 and wpi6 (Breton et al 2003;Imai et al 2005).…”

Section: Introductionmentioning

confidence: 97%

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The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

Hong

Hwang

2008

Planta

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The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 was analyzed by an Agrobacterium-mediated transient expression assay in tobacco leaves. Several stress-related cis-acting elements (GT-1, W-box and ABRE) are located within the CaPIMP1 promoter. In tobacco leaf tissues transiently transformed with a CaPIMP1 promoter-beta-glucuronidase (GUS) gene fusion, serially 5'-deleted CaPIMP1 promoters were differentially activated by Pseudomonas syringae pv. tabaci, ethylene, methyl jasmonate, abscisic acid, and nitric oxide. The -1,193 bp region of the CaPIMP1 gene promoter sequence exhibited full promoter activity. The -417- and -593 bp promoter regions were sufficient for GUS gene activation by ethylene and methyl jasmonate treatments, respectively. However, CaPIMP1 promoter sequences longer than -793 bp were required for promoter activation by abscisic acid and sodium nitroprusside treatments. CaPIMP1 expression was activated in pepper leaves by treatment with ethylene, methyl jasmonate, abscisic acid, beta-amino-n-butyric acid, NaCl, mechanical wounding, and low temperature, but not with salicylic acid. Overexpression of CaPIMP1 in Arabidopsis conferred hypersensitivity to mannitol, NaCl, and ABA during seed germination but not during seedling development. In contrast, transgenic plants overexpressing CaPIMP1 exhibited enhanced tolerance to oxidative stress induced by methyl viologen during germination and early seedling stages. These results suggest that CaPIMP1 expression may alter responsiveness to environmental stress, as well as to pathogen infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

Hong

Hwang

2008

Planta

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“…The binding of the nsLTP to the receptor induces plant resistance to abiotic and biotic stresses (Jung et al 2005). BLT101 (barley lipid transfer protein) and orthologues have been isolated and studied in several plants, including Arabidopsis, moss, wheat, rice, tobacco, barley, and yeast (Goddard et al 1993;Capel et al 1997;Brown 1998 Park andYu 2000;Medina et al 2001;Nylander et al 2001;Imai et al 2005;Morsy et al 2005;Feng et al 2009). Pmp1P, Pmp2P, and Pmp3P (plasma membrane H ?…”

Section: Introductionmentioning

confidence: 97%

“…Therefore, the plants need to enhance the cryostability of the plasma membrane, during the cold acclimation process, in order to minimize the freezing damage. The lipid composition of the plasma membrane is altered in several plant species, during cold acclimation (Uemura et al 1995;Imai et al 2005); however, there are only a few studies regarding the proteins accumulated in the plasma membrane of crop plants during cold acclimation (Zhou et al 1994;Imai et al 2005;Takahashi et al 2013). …”

Section: Introductionmentioning

confidence: 98%

“…In the moss (Physcomitrella patens), PpSHP1 and PpSHP2 were induced as a BLT101 orthologue by cold, ABA, salt, osmoticum, and dehydration. TaWPI6 and OsLti6, BLT101 orthologues in cereal plants were induced by many stresses and ABA, and the encoded proteins were located in the plasma membrane (Imai et al 2005;Morsy et al 2005). BLT101, from barley, was up-regulated by cold (Goddard et al 1993;Brown 1998).…”

Section: Introductionmentioning

confidence: 99%

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The barley lipid transfer protein, BLT101, enhances cold tolerance in wheat under cold stress

Choi

Hwang²

2015

Plant Biotechnol Rep

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To understand the mechanism by which the barley plants tolerate overwintering, we analyzed the BLT101 gene expression in four different cultivars including two each of spring and winter barleys, where the BLT101 transcript level varied among the cultivars with a long-term cold acclimation. The BLT101 transcript was induced at a higher level in the crown tissues in response to low temperature treatment. The BLT101 transcript level correlated with those of the corresponding protein. BLT101-overexpressing transgenic wheat lines (BLT101ox's) grew slowly and showed decreased expression of the genes induced by hormones (such as auxin and cytokinin), compared to non-transgenic (NT) plants. After a prolonged cold treatment, the BLT101ox leaves showed normal phenotypes, while the NT plants displayed blighted and withered leaves. A substances leakage test showed that the BLT101ox wheat retains enhanced freezing tolerance, compared to the NT plants. The transgenic lines lost less water than the NT plants, under a cold-stressed condition. Our findings suggest that tolerance to water stress and the retardation of growth in cold conditions, the essential features in overwintering barleys, involve BLT101.

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Gene Expression Patterns in Near Isogenic Lines for Wheat Rust Resistance Gene Lr34/Yr18

Hulbert¹,

Bai²,

Fellers³

et al. 2007

Phytopathology®

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The Lr34/Yr18 resistance gene provides durable, adult-plant, slow rusting resistance to leaf rust, yellow rust, and several other diseases of wheat. Flag leaves may exhibit spontaneous leaf tip necrosis and tips are more resistant than leaf bases. Despite the importance of this gene, the mechanism of resistance is unknown. Patterns of expression for 55,052 transcripts were examined by microarray analysis in mock-inoculated flag leaves of two pairs of wheat near isogenic lines for Lr34/Yr18 (Jupateco 73S/Jupateco 73R and Thatcher/Thatcher-Lr34). The Thatcher isolines were also examined for patterns of expression after inoculation with leaf rust. Mock-inoculated leaf tips of resistant plants showed up-regulation of 57 transcripts generally associated with ABA inducibility, osmotic stress, cold stress, and/or seed maturation. Several transcripts may be useful as expression markers for Lr34/Yr18. Five transcripts were also up-regulated in resistant leaf bases. The possible role of these transcripts in resistance is discussed. In mock-inoculated plants, pathogenesis-related (PR) proteins were not up-regulated in resistant flag leaves compared with that in susceptible flag leaves. In inoculated plants, the same set of PR proteins was up-regulated in both resistant and susceptible flag leaves. However, expression was often higher in resistant plants, suggesting a possible role for Lr34/Yr18 in priming of defense responses.

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Molecular characterization of a cold-induced plasma membrane protein gene from wheat

Abstract: The online version of the original article can be found at http:// dx

Cited by 5 publications

References 0 publications

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

The barley lipid transfer protein, BLT101, enhances cold tolerance in wheat under cold stress

Gene Expression Patterns in Near Isogenic Lines for Wheat Rust Resistance Gene Lr34/Yr18

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