Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish ( Sebastes schlegelii ): Deciphering its putative antibacterial role

Elvitigala, Don Anushka Sandaruwan; Lee, Sukkyoung; Kim, Hyun-Chul; Park, Hae Chul; Lee, Jehee

doi:10.1016/j.dci.2016.09.011

Cited by 14 publications

(12 citation statements)

References 38 publications

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“…This antimicrobial effector selectively binds to LPS, determining the specificity of recognition for Gram-negative bacteria, and leads to bacterial cell lysis 75 . The conserved antimicrobial function of fish BPIs, demonstrated by multiple studies 76–78 , implies that its induction in salmon might provide increased protection against Gram-negative bacterial infection. This activity could be complementary to that of lysozyme, expected to confer increased protection against Gram-positive bacterial infections.…”

Section: Resultsmentioning

confidence: 99%

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Jalili

Jin

Bones

et al. 2019

Sci Rep

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The quality and relative amounts of dietary lipids may affect the health and growth of cultured Atlantic salmon. So far, little is known about their effects on the performance of the fish immune system during early life stages and, in particular their importance in the transition from endogenous nutrition (yolk) in the alevin stage to exogenous nutrition in the later fry stage. We investigated the immunomodulatory effects of fish oil, vegetable oil and phospholipid-rich oil in feeds for farmed Atlantic salmon using a transcriptomic approach. The experiment allowed a fine-scale monitoring of gene expression profiles in two tissues, the pyloric caeca of the intestine and the liver, in a 94 days-long first feeding experiment. The analysis of transcriptional profiles revealed that first feeding induced a strong immunomodulation in the pyloric caeca after 48 days of feeding, lasting up to day 94 and possibly beyond. On the other hand, the differential effect of the three dietary regimes was negligible. We interpret this upregulation, undetectable in liver, as a potentiation of the immune system upon the first contact of the digestive system with exogenous feed. This process involved a complex network of gene products involved in both cellular and humoral immunity. We identified the classical pathway of the complement system, acting at the crossroads between innate and adaptive immunity, as a key process modulated in response to the switch from endogenous to exogenous nutrition.

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Section: Resultsmentioning

confidence: 99%

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Jalili

Jin

Bones

et al. 2019

Sci Rep

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show abstract

“…Therefore, we hypothesized that the affinity towards LPS is roughly determined by the respective quantity of basic amino acids. Preserved LPS neutralization as well as bactericidal activity was previously described for BPI orthologues derived from distantly related species such as the Pacific oyster (23,24) and ray finned fish (Actinopterygii; 22,27). Upon 10 published sequences of Actinopterygii BPI, scoBPI comprised the highest number of cationic arginine, histidine, and lysine amino acids in the corresponding regions (Fig.…”

Section: Comparison Of the N-terminal Barrel In Orthologous Bpimentioning

confidence: 80%

“…2C). Given previous publications on bactericidal activity (21)(22)(23)(24), we selected muBPI, scoBPI and osBPI for functional analysis. After recombinant expression of huBPI, muBPI, scoBPI and osBPI (Fig.…”

Section: No Recognition Of Scobpi By Bpi-anca From Cf Patientsmentioning

confidence: 99%

“…To restore BPI function, we speculated that orthologous proteins with conserved bactericidal and LPS-neutralizing activity would escape recognition by intrinsic BPI-ANCA and therefore preserve their bactericidal activity when applied to BPI-ANCA positive CF patients. Based on known properties, sequence homologies and structural considerations we selected murine BPI (muBPI) of the house mouse Mus musculus (21), scorpionfish BPI (scoBPI) of the Korean rockfish Sebastes schlegelii (22) and oyster BPI (osBPI) of Magallana gigas (Pacific oyster, previously Crassostrea gigas; 23,24) for comparison to human BPI (huBPI, Homo sapiens). In contrast to huBPI, neither muBPI nor scoBPI were recognized by BPI-ANCA present in the sera of CF patients.…”

Section: Introductionmentioning

confidence: 99%

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Scorpionfish BPI is highly active against multiple drug-resistantPseudomonas aeruginosaisolates from cystic fibrosis patients

Holzinger

Toelge

Werner

et al. 2023

Preprint

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Chronic pulmonary infection is a hallmark of cystic fibrosis (CF) and requires continuous antibiotic treatment. In this context, Pseudomonas aeruginosa (Pa) is of special concern since colonizing strains frequently acquire multiple drug resistance (MDR). Bactericidal/permeability-increasing protein (BPI) is a neutrophil-derived, endogenous protein with high bactericidal potency against Gram-negative bacteria. However, a significant range of CF patients produce anti-neutrophil cytoplasmic antibodies against BPI (BPI-ANCA) thereby neutralizing its bactericidal function. In accordance with literature, we describe that 54.3% of a total of 46 CF patients expressed BPI-ANCA. Importantly, an orthologous protein to human BPI (huBPI) derived from the scorpionfish Sebastes schlegelii (scoBPI) completely escaped recognition by these autoantibodies. Moreover, scoBPI exhibited high anti-inflammatory potency towards Pa LPS, and was bactericidal against MDR Pa derived from CF patients at nanomolar concentrations. In conclusion, our results highlight the potential of highly active orthologous proteins of huBPI in treatment of MDR Pa infections, especially in the presence of BPI-ANCA.

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“…RbBPI/LBPI which belongs to the family of AMPs (Antimicrobial proteins) has been isolated from a wide range of teleost fishes. The characterization and differential expression of RbBPI/LBPI in response to several immune-stimulants and bacteria has been studied (Lee et al, 2017). The up-regulation of RbBPI/LBPI in E. suratensis indicates their role in the immune system.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic approach to study salinity tolerance in euryhaline cichlid, Etroplus suratensis (Bloch, 1790)

Kumar¹,

Pradeep²,

Arshad³

et al. 2020

J. Mar. Biol. Ass. India

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Increasing levels of salinity in coastal and freshwater environments as a result of rise in sea level is linked to climate change and it impacts fish growth adversely. Etroplus suratensis (Pearlspot) is the largest species among the indigenous cichlid which is essentially brackishwater fish that has become naturally acclimated to freshwater. Understanding the basis of tolerance and acclimatization to increased environmental salinity and freshwater condition can help the regional aqua-farmers to mitigate predicted impacts of climate change in that region. Technically the last decade contributed many methodologies to understand the salinity adaptation mechanisms; suppression subtractive hybridization (SSH) library generation being one of them. In this study we performed SSH technique to identify genes involved in salinity adaptation and osmoregulation in gills of E. suratensis, generating from fishes exposed to seawater (SW-36 ‰) as compared to freshwater (FW-0 ‰). Here, SSH was used to identify alterations in gene transcription of E. suratensis after exposure to 36‰. A random 105 clones were selected and sanger sequenced from the subtractive c-DNA libraries which gave a total of 80 gene fragment sequences. Sequencing analysis and homology searches showed that these EST's represented 68 unique gene and 12 duplicates. Of the 68 unique genes, 62 shared high homology with fish genes of known functions including immunity, stress, cellular process, cytoskeleton and transporter.Gene expression profile of the selected 10 EST's acclimated to SW and FW conditions from SSH library was carried out. The candidate genes identified in E. suratensis gills provided valuable information to study and understand new functional genes that act as osmosensors in osmotic regulations.

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Molecular characterization of a bactericidal permeability-increasing protein/lipopolysaccharide-binding protein from black rockfish ( Sebastes schlegelii ): Deciphering its putative antibacterial role

Cited by 14 publications

References 38 publications

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Scorpionfish BPI is highly active against multiple drug-resistantPseudomonas aeruginosaisolates from cystic fibrosis patients

Transcriptomic approach to study salinity tolerance in euryhaline cichlid, Etroplus suratensis (Bloch, 1790)

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