Molecular characterization of a 10‐kDa buckwheat molecule reactive to allergic patients’ IgE

Matsumoto, Reiko; Fujino, Kazuo; Nagata, Yukihiro; Hashiguchi, Shuhei; Ito, Yuji; Aihara, Yukoh; Takahashi, Yuriko; Maeda, Keiji; Sugimura, Kazuhisa

doi:10.1046/j.1398-9995.2003.00412.x

Cited by 41 publications

(34 citation statements)

References 21 publications

(25 reference statements)

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“…Shared IgE epitopes are not unique to 19-kD allergen. Marked homology and shared IgE epitopes between 16- and 10-kD BW allergens, which belong to the 2 S storage protein, were also found [14]. …”

Section: Discussionmentioning

confidence: 99%

“…The 24-kD BW protein was identified as the β-subunit of 11 S globulin [8,9,10], the 9-kD BW allergen as vicilin [11,12,13] and the 10-kD BW protein as 2 S albumin [14]. Specific IgE (sIgE) antibodies to the 24- and 9-kD allergens can be found in both BW-allergic and asymptomatic subjects, making these allergens poor candidate biomarkers for diagnosis of BW reactivity [13, 15].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

Choi

Sohn

Lee

et al. 2007

Int Arch Allergy Immunol

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Background: The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. Methods: cDNA of the 19-kD BW allergen was cloned and expressed in Escherichia coli. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. Results: The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew (Ana o 1), English walnut (Jug r 2) and 7 S globulin from Sesamum indicum. The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839–1.012), p < 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725–0.995), p <0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661–0.945), p = 0.002]. Conclusions: The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

Choi

Sohn

Lee

et al. 2007

Int Arch Allergy Immunol

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“…2). Matsumoto et al [10] reported that the 10-kDa buckwheat protein, which is identical to the 8-kDa buckwheat allergen, is a member of the 2S albumin multigene family. 2S albumin family proteins have been identified as vacuolar-targeting storage proteins in many plants, and their N-terminal amino acid sequences include signal peptides that are absent in the mature proteins [15,16,17,18].…”

Section: Resultsmentioning

confidence: 99%

“…For instance, 24-, 19-, 16- and 9-kDa proteins have been identified as major buckwheat allergens [7,8,9]. Furthermore, Matsumoto et al [10] have reported a 10-kDa protein and its allergenicity. The 10-kDa protein, which is identical to an 8-kDa buckwheat allergen (Genbank accession No.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning of cDNA, Recombinant Protein Expression and Characterization of a Buckwheat 16-kDa Major Allergen

Koyano

Takagi

Teshima

et al. 2006

Int Arch Allergy Immunol

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Background: Buckwheat is a common food in Japan, Korea and other countries. A candidate major buckwheat allergen, a 16-kDa protein (BWp16), was previously characterized as a pepsin-resistant protein associated with immediate-type allergies to buckwheat. However, whether recombinant BWp16 can react with a patient’s IgE remains uncertain. Methods: The cDNA encoding BWp16 from Japanese buckwheat seeds was cloned based on the sequences obtained by the 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE PCR. Recombinant BWp16 protein expressed in Escherichia coli was purified using affinity chromatography. Western blotting, ELISA and cross inhibition tests of the purified recombinant BWp16 were performed using sera from patients with positive IgE binding to buckwheat and controls. Pepsin digestion experiments were also performed. Results: The full-length cDNA encodes 149 amino acid residues with a calculated molecular mass of 16.9 kDa. The deduced amino acid sequence included a putative signal peptide sequence. BWp16 showed significant homologies to the buckwheat 8-kDa allergen and Ricinus communis (castor bean) 2S albumin. Sera from patients with positive IgE binding to buckwheat reacted with the purified BWp16. Cross inhibition tests revealed immunological equivalence of the purified recombinant and natu ral BWp16. The recombinant and natural BWp16 were comparably resistant to pepsin digestion. Conclusions: BWp16 belongs to the 2S albumin family and is a buckwheat allergen. This purified recombinant BWp16 could be used in the diagnosis of buckwheat allergy.

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“…The most represented proteins are the so called "storage proteins" and in particular 13S globulins [30]. Several buckwheat proteins are described as being able to bind IgE in allergic patients, and proteins with molecular weight of 9, 16, 19, and 24 kDa are considered major allergens [31][32][33][34][35] (Table 1). It has recently been suggested that sensitization to specific buckwheat allergens would be related to specific symptoms, leading to three different clinical patterns.…”

Section: Buckwheat Allergens and Known Cross-reactivitiesmentioning

confidence: 99%

Buckwheat Allergy: An Emerging Clinical Problem in Europe

Heffler¹,

Pizzimenti²,

Badiu³

et al. 2014

J Allergy Ther

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Buckwheat allergy is a clinical entity known since long time ago and frequent in Asia, where this crop is commonly eaten. In Europe, buckwheat allergy was an anedoctical description since few years ago, when the consumption of buckwheat dramatically increased together with the publication of case reports and case series of European patients with buckwheat allergy.This review article describes and analyzes the history of buckwheat allergy, its clinical presentation, its increase in European countries during the last few years, and summarizes the main case reports, case series and epidemiological surveys on buckwheat allergy.A summary of main buckwheat allergens and clinical relevant cross-reactivity are also described and discussed.

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Molecular characterization of a 10‐kDa buckwheat molecule reactive to allergic patients’ IgE

Abstract: Molecular cloning experiments indicated that BW10KD as a BW allergen was a member of the 2S-albumin multigene family.

Cited by 41 publications

References 21 publications

Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

Characterization of Buckwheat 19-kD Allergen and Its Application for Diagnosing Clinical Reactivity

Molecular Cloning of cDNA, Recombinant Protein Expression and Characterization of a Buckwheat 16-kDa Major Allergen

Buckwheat Allergy: An Emerging Clinical Problem in Europe

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