Molecular characterization of 5-chlorophyll a/b-binding protein genes from Panax ginseng Meyer and their expression analysis during abiotic stresses

Abstract

The chlorophyll a/b-binding protein (CAB) serves in both photosystems (PS), I and II, as a coordinator of antenna pigments in the light-harvesting complex (LHC). The CABs constitute abundant and important proteins in the thylakoid membrane of higher plants. In our study, five CAB genes, which contained full-length cDNA sequences from the 4-yearold ginseng leaves (Panax ginseng Meyer), were isolated and named PgCAB. Phylogenetic comparison of the members of the subfamily between ginseng and higher plants, including Arabidopsis, revealed that the putative functions of these ginseng CAB proteins were clustered into the different family of Arabidopsis CABs; two PgCABs in LHCII family and three PgCABs in LHCI family. The expression analysis of PgCABs consistently showed dark-dependent inhibition in leaves. Expression analysis during abiotic stress identified that PgCAB genes responded to heavy metal, salinity, chilling, and UV stresses differently, suggesting their specific function during photosynthesis. This is the first comprehensive study of the CAB gene family in P. ginseng.

Comparative chloroplast proteome analysis of exogenously supplied trehalose to wheat seedlings under heat stress

“…Previously, we found that the proteins in the thylakoid membranes and photosynthetic capacity damaged by heat stress could be protected by Tre pretreatment in wheat seedlings (Luo et al 2010). However, in this study, when exposed to heat stress, the level of CAB, which serves to maximize and regulate light harvesting (Silva et al 2016), was upregulated (Fig. 3D, 3.19 ± 0.19).…”

“…3D, 3.19 ± 0.19). Silva et al (2016) reported that the PgCAB1 and PgCAB2 expression levels significantly increased compared with control under NaCl stress, which suggested the specific function of PSII in the regulatory role in ginseng response to salinity and drought and this also might represent a strategy to prevent light stress-induced damage. We speculated that the plant itself might depress its energy supply to photosynthesis because of the heat stress and thus hope to meet the needs of energy and its metabolic balance by enhancing light harvesting in order to respond to this decrease in photosynthesis.…”

Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment

“…In a previous report, an increase in the expression of the CAB gene caused the accumulation of chlorophyll and stability in the chloroplast thylakoid membranes in green tomato fruit [47]. Silva et al [48] found that the expression of CABs is affected by abiotic stress, which indicated the CAB gene plays an important role in abiotic resistance. Peralta et al [49] reported that with an increase in the expression levels of CAB1, there is a lack of yellowing in rosette leaves, and therefore, the increase in the expression level delays leaf senescence.…”

Comparative Proteomic Analysis Reveals That the Heterosis of Two Maize Hybrids is Related to Enhancement of Stress Response and Photosynthesis Respectively

“…CABs are a class of important proteins in thylakoid membranes of higher plants, and take part in both photosystems I and II. In addition, CABs may be involved in abiotic stress responses [55]. It was also found that CABs were highly expressed in the F1 hybrid of Chinese cabbage at two days after sowing, means that early developmental events in the germinating seedling of the hybrid may be important for later developmental vigor and yield advantage [56].…”