Molecular characterization, fitness and mycotoxin production of Fusarium graminearum laboratory strains resistant to benzimidazoles

Sevastos, A.; Markoglou, Anastasios N.; Labrou, Nikolaos E.; Flouri, F.; Malandrakis, Anastasios A.

doi:10.1016/j.pestbp.2015.10.004

Cited by 21 publications

(7 citation statements)

References 40 publications

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“…In recent years, food safety has become an important topic, and synthetic fungicides have been shown to cause serious health problems in humans [38][39][40]. Moreover, F. graminearum has developed resistance to synthetic fungicides, such as azoles and carbendazim, because of their long-term usage [41][42][43][44][45]. Therefore, it is of great significance to develop active ingredients of biological fungicides to combat this critical pathogen.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

et al. 2022

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Kiwifruit (Actinidia chinensis) is an important commercial crop in China, and the occurrence of diseases may cause significant economic loss in its production. In the present study, a new pathogen that causes brown leaf spot disease on kiwifruit was reported. The fungus was isolated from an infected sample and identified as Fusarium graminearum based on morphological and molecular evaluation. Koch’s postulates were confirmed when the pathogen was re-isolated from plants with artificially induced symptoms and identified as F. graminearum. Based on the biological characteristics of the pathogen, it was determined that: its optimal growth temperature was 25 °C; optimal pH was 7; most suitable carbon source was soluble starch; most suitable nitrogen source was yeast powder; and best photoperiod was 12 h light/12 h dark. Further investigations were conducted by determining 50% effective concentrations (EC50) of several active ingredients of biological fungicides against F. graminearum. The results showed that among the studied fungicides, tetramycin and honokiol had the highest antifungal activity against this pathogen. Our findings provide a scientific basis for the prevention and treatment of brown leaf spot disease on kiwifruit.

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Section: Discussionmentioning

confidence: 99%

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

et al. 2022

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“…Carbendazim-resistant mutants showed rapid growth in a fan-shaped region on the edge of the colony. The resistant phenotype strains in the fan-shaped region were transferred to fungicide-free PDA medium and continuously sub-cultured for 10 generations [18, 28]. The sensitivity of resistant strains was measured as described above.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease

Wang

et al. 2019

BMC Genomics

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BackgroundPokkah boeng is one of the most serious and devastating diseases of sugarcane and causes significant loss in cane yield and sugar content. Although carbendazim is widely used to prevent fungal diseases, the molecular basis of Fusarium species complex (FSC) resistance to carbendazim remains unknown.ResultsThe EC50 (fungicide concentration that inhibits 50% of mycelial growth) values of carbendazim for 35 FSC isolates collected in cane growing regions of China were ranged from 0.5097 to 0.6941 μg mL− 1 of active ingredient (a.i.), in an average of 0.5957 μg a.i. mL− 1. Among carbendazim-induced mutant strains, SJ51M (F. verticillioides) had a CTG rather than CAG codon (Q134L) at position 134 of the FVER_09254 gene, whereas in the mutant strain HC30M (F. proliferatum) codon ACA at position 351 of the FPRO_07779 gene was replaced by ATA (T351I). Gene expression profiling analysis was performed for SJ51M and its corresponding wild type strain SJ51, with and without carbendazim treatment. The gene expression patterns in SJ51 and SJ51M changed greatly as evidenced by the detection of 850 differentially expressed genes (DEGs). Functional categorization indicated that genes associated with oxidation-reduction process, ATP binding, integral component of membrane, transmembrane transport and response to stress showed the largest expression changes between SJ51M and SJ51. The expression levels of many genes involved in fungicide resistance, such as detoxification enzymes, drug efflux transporters and response to stress, were up-regulated in SJ51M compared to SJ51 with and without carbendazim treatment.ConclusionFSC was sensitive to carbendazim and had the potential for rapid development of carbendazim resistance. The transcriptome data provided insight into the molecular pathways involved in FSC carbendazim resistance.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5479-6) contains supplementary material, which is available to authorized users.

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“…Considering the high impact of FBs and DON on human and animal health, and the economic losses related with the contamination of maize and wheat grains with mycotoxins, it is important to develop strategies in order to prevent their formation and/or to eliminate, inactivate, or reduce their presence in both cereals and food products. For this, several strategies such as a combination of agronomical practices, resistant cultivars, and fungicides are used [27]. However, chemical fungicides are reported to cause problems such as ambient pollution, chemical residues in food and feed, and the development of resistant pathogenic fungi [28].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Study on the Use of Chitosan as an Eco-Friendly Alternative to Control Fusarium Growth and Mycotoxin Production on Maize and Wheat

et al. 2019

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The objectives of the present study were to determine the combined effects of chitosan and water activity (aW) on growth and mycotoxin production in situ on the two most important Fusarium species (F. proliferatum and F. verticillioides) present on maize, and on F. graminearum, the main pathogen causing Fusarium head blight on wheat. Results showed that low-molecular-weight chitosan with more than 70% deacetylation at the lowest dose used (0.5 mg/g) was able to reduce deoxynivalenol (DON) and fumonisin (FBs) production on irradiated maize and wheat grains. Growth rates of F. graminearum also decreased at the lowest chitosan dose used (0.5 mg/g), while F. verticillioides and F. proliferatum growth rates were reduced at 0.98 aW at the highest chitosan dose used (2 mg/g). Since mycotoxins are unavoidable contaminants in food and feed chains, their presence needs to be reduced in order to minimize their effects on human and animal health and to diminish the annual market loss through rejected maize and wheat; in this scenario, pre- and post-harvest use of chitosan could be an important alternative.

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Molecular characterization, fitness and mycotoxin production of Fusarium graminearum laboratory strains resistant to benzimidazoles

Cited by 21 publications

References 40 publications

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease

Preliminary Study on the Use of Chitosan as an Eco-Friendly Alternative to Control Fusarium Growth and Mycotoxin Production on Maize and Wheat

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