Molecular characterization, expression pattern, and function analysis of the OsBC1L family in rice

Dai, Xiaoxia; You, Changjun; Wang, Lei; Chen, Guoxing; Zhang, Qifa; Wu, Changyin

doi:10.1007/s11103-009-9537-3

Cited by 32 publications

(32 citation statements)

References 48 publications

(66 reference statements)

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“…The PCR primers that flanked the T-DNA insertion site were designed for genotyping analysis according to the method described by Dai et al (2009). The PCR was performed in an ABI 9700 thermocycler (Applied Biosystems, Foster City, CA, USA) with the following cycling profile: 94°C for 5 min, followed by 28 cycles of 94°C for 45 s, 55°C for 45 s, and 72°C for 1 min, and a final 5-min extension at 72°C.…”

Section: Cell Wall Composition Analysismentioning

confidence: 99%

“…AtCOBL9 and ZmBk2L1 are required for tip-directed growth in root hair development in Arabidopsis and maize, respectively (Parker et al 2000;Jones et al 2006;Hochholdinger et al 2008). The molecular characterization, expression pattern, and phenotype variations of knockout mutants of OsBC1L genes have been reported in rice (Dai et al 2009). Generally, the Osbc1l4 (Oryza sativa brittle culm 1 like 4) mutants exhibited a dwarf phenotype with fewer tillers, while Osbc1l5 may cause a defect in pollen germination (Dai et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The molecular characterization, expression pattern, and phenotype variations of knockout mutants of OsBC1L genes have been reported in rice (Dai et al 2009). Generally, the Osbc1l4 (Oryza sativa brittle culm 1 like 4) mutants exhibited a dwarf phenotype with fewer tillers, while Osbc1l5 may cause a defect in pollen germination (Dai et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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OsBC1L4 encodes a COBRA-like protein that affects cellulose synthesis in rice

et al. 2011

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Plant morphogenesis is highly dependent on the regulation of cell division and expansion. The organization of the cellulose microfibrils in the cell wall is a key determinant of cell expansion. Previously, a dwarf mutant with fewer tillers, Osbc1l4 (Oryza sativa brittle culm 1 like 4), was identified by screening a rice T-DNA insertion mutant library. It is reported here that OsBC1L4 encodes a COBRA-like protein that exhibits typical structural features of a glycosylphosphatidylinositol-anchor protein. The T-DNA insertion in OsBC1L4 results in abnormal cell expansion. A decrease in cellulose content but the increase in pectin and starch contents was identified in Osbc1l4 mutants by measuring the content of wall components. OsBC1L4 was expressed in all tissues/organs examined, with a low level in leaves. OsBC1L4 protein is mainly located in the cell wall and plasma membrane. Correlation analysis indicated that the expression of OsBC1L4 was highly correlated to that of several primary wall-forming cellulose synthase genes (CESAs). Moreover, the expression level of several cellulose-related genes is increased in Osbc1l4 mutants, which suggests that a feedback mechanism may exist during cellulose synthesis.

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Section: Cell Wall Composition Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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OsBC1L4 encodes a COBRA-like protein that affects cellulose synthesis in rice

et al. 2011

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“…6, 7). In rice, CWA1/BC1 belongs to an 11-member multigene family, and co-expressed of seven BC1-like genes including CWA1/BC1 in the internodes has been reported (Dai et al 2009). Some of the 12 members of the COBRA family, including COBL4, are also known to be co-expressed in Arabidopsis leaf vascular bundles and to be involved in vascular bundle formation (Roudier et al 2002;Brady et al 2007), while Bk2 is similarly co-expressed with some Bk2-like genes in various tissues in maize (Brady et al 2007).…”

Section: Cwa1 Encodes Gpi-anchored Cobra-like Proteinmentioning

confidence: 99%

“…COBRA belongs to a plant-speciWc multigene family; there are 12 members in Arabidopsis (Roudier et al 2002;Brady et al 2007), 11 in rice (Brady et al 2007;Dai et al 2009), and 9 in maize (Brady et al 2007). A mutation in the COBRA gene in Arabidopsis results in defective cellulose synthesis and cellulose microWbril orientation in its roots, aVecting the orientation of cell expansion in its root elongation zone (Schindelman et al 2001;Roudier et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Isolation of a novel cell wall architecture mutant of rice with defective Arabidopsis COBL4 ortholog BC1 required for regulated deposition of secondary cell wall components

Sato

Suzuki

Nishikubo

et al. 2010

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The plant secondary cell wall is a highly ordered structure composed of various polysaccharides, phenolic components and proteins. Its coordinated regulation of a number of complex metabolic pathways and assembly has not been resolved. To understand the molecular mechanisms that regulate secondary cell wall synthesis, we isolated a novel rice mutant, cell wall architecture1 (cwa1), that exhibits an irregular thickening pattern in the secondary cell wall of sclerenchyma, as well as culm brittleness and reduced cellulose content in mature internodes. Light and transmission electron microscopy revealed that the cwa1 mutant plant has regions of local aggregation in the secondary cell walls of the cortical fibers in its internodes, showing uneven thickness. Ultraviolet microscopic observation indicated that localization of cell wall phenolic components was perturbed and that these components abundantly deposited at the aggregated cell wall regions in sclerenchyma. Therefore, regulation of deposition and assembly of secondary cell wall materials, i.e. phenolic components, appear to be disturbed by mutation of the cwa1 gene. Genetic analysis showed that cwa1 is allelic to brittle culm1 (bc1), which encodes the glycosylphosphatidylinositol-anchored COBRA-like protein specifically in plants. BC1 is known as a regulator that controls the culm mechanical strength and cellulose content in the secondary cell walls of sclerenchyma, but the precise function of BC1 has not been resolved. Our results suggest that CWA1/BC1 has an essential role in assembling cell wall constituents at their appropriate sites, thereby enabling synthesis of solid and flexible internodes in rice.

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Molecular characterization and functional analysis of “fruit-weight2.2-like” gene family in rice

Xiong

Cao

et al. 2013

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Tomato fruit-weight 2.2 (FW2.2) was reported to control up to 30 % fruit weight. Recent studies demonstrated that FW2.2-like (FWL) genes also play important roles in plant growth and development. For instance, a maize homolog of FW2.2, named cell number regulator 1 (CNR1), negatively regulates plant and organ size. However, FWL genes in rice have not been characterized yet. In this study, eight FWL genes were identified in rice genome and designated as OsFWL1-8. The chromosome location, gene structure, protein motif, and phylogenetic relationship of OsFWL genes were analyzed. RT-PCR result and microarray data revealed that OsFWL genes exhibited diverse expression patterns and the detailed expression patterns of OsFWL5, 6, and 7 negatively correlated with leaf growth activity. Rice protoplast transient transformation experiment showed that most OsFWL proteins locate at cell membrane but OsFWL8 is present in the nucleus. In addition, the functions of OsFWL genes were investigated by analyzing two T-DNA insertion lines for OsFWL3 and 5. Compared with wild type, the grain weight of osfwl3 mutant and the plant height of osfwl5 mutant were increased by 5.3 and 12.5 %, respectively. We also found that the increase in grain length of osfwl3 mutant was due chiefly to incremental cell number, not cell size and the expression of OsFWL3 negatively correlated with glume growth activity. These results provide a comprehensive foundation for further study of OsFWL functions in rice.

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Molecular characterization, expression pattern, and function analysis of the OsBC1L family in rice

Cited by 32 publications

References 48 publications

OsBC1L4 encodes a COBRA-like protein that affects cellulose synthesis in rice

OsBC1L4 encodes a COBRA-like protein that affects cellulose synthesis in rice

Isolation of a novel cell wall architecture mutant of rice with defective Arabidopsis COBL4 ortholog BC1 required for regulated deposition of secondary cell wall components

Molecular characterization and functional analysis of “fruit-weight2.2-like” gene family in rice

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