Molecular characterization, expression and response to immune challenges of 3 members of the toll-like receptor superfamily 11 in the golden pompano (Trachinotus ovatus)

Da, Fan; Tan, Huiming; Wan, Xiaoju; Lin, Guixiang; Jian, Jichang; Wen, Zhengyong; Cai, Shuanghu

doi:10.1016/j.aqrep.2022.101268

Cited by 2 publications

(10 citation statements)

References 28 publications

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“…In the present study, when bullfrogs were challenged with microbial pathogens and the expression of LcTLR13 genes in the spleen and liver was analyzed to determine their role in immune response, mRNA transcript levels of LcTLR13a, LcTLR13b, and LcTLR13c decreased during early time points of the infection, which is similar to the expression of TLR13 paralogs in the spleen and kidney of T. ovatus after LPS and Poly I:C challenge (Da et al, 2022). However, when bullfrogs were challenged with the pathogens for 24 and 48 h, the expression levels of LcTLR13a, LcTLR13b, and LcTLR13c were significantly increased in the spleen and liver.…”

Section: Discussionmentioning

confidence: 55%

“…And TLR13 homologs have been reported in several teleosts, including N. albiflora (Y. Liu et al, 2023), T. ovatus (Da et al, 2022), E. coioides (Liang et al, 2018), A. dabryanus (Tang et al, 2020), and O. niloticus (F. Y. Gao, Pang, et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Although in mice only one copy of TLR13 has been reported, multiple homologs of TLR13 are found in L. maculatus (Fan et al, 2019), O. niloticus (F. Y. Gao, Pang, et al, 2021), S. chuatsi (K. L. Wang et al, 2021), and T. ovatus (Da et al, 2022). As previous study showed, the TLR genes exist in large number of duplicate genes in teleost fish; for example, TLR8a1 and TLR8a2 were identified in O. mykiss (Palti et al, 2010), and TLR7a-e, TLR8a-e, and TLR9a-e were identified in Gadus morhua (Star et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity

Yang,

Aweya,

Guo

et al. 2023

J World Aquaculture Soc

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Toll‐like receptors (TLRs) are an important family of pattern‐recognition receptors that recognize a series of pathogen‐associated molecular patterns to trigger the host immune response. In the present study, we identified three TLR13 genes (i.e., LcLTR13a, LcTLR13b, and LcTLR13c) in American bullfrogs, Lithobates catesbeianus, and explored their constitutive and pathogen‐induced expression profile in different tissues. The open reading frames of LcLTR13a, LcTLR13b and LcTLR13c are 2811, 2844, and 2814 bp long, encoding 955, 947, and 933 amino acids, respectively. Phylogenetic analysis showed that three LcTLR13 genes grouping in different subclades indicate the gene duplication of bullfrog TLR13 as in teleost. The three LcTLR13 genes were expressed in all the tissues examined, with the liver and spleen expressing the higher levels. Moreover, the expression levels of LcLTR13a, LcTLR13b, and LcTLR13c were significantly induced in the liver and spleen after bullfrogs were challenged with bacterial (i.e., Citrobacter freundii, Aeromonas hydrophila, and Edwardsiella tarda) pathogens. These results indicate that LcTLR13 genes play an essential role in the bullfrog antibacterial innate immune response, providing new insights into immune mechanisms of bullfrogs.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity

Yang,

Aweya,

Guo

et al. 2023

J World Aquaculture Soc

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“…Golden pompano (Trachinotus ovatus) is widely aquacultured for its rapid growth speed, high disease resistance, and superior nutritional value, and has become one of the essential marine economic fish (Zhu et al, 2019;Da et al, 2022). In this study, we identified two paralogs of caspase-8 in golden pompano using the same method as in previous studies (Da et al, 2022).…”

Section: Introductionmentioning

confidence: 89%

“…Each experiment was repeated three times. The relative mRNA expression level was calculated by the 2 −DDCT method (Da et al, 2022).…”

Section: Quantitative Real-time Reverse Transcriptase-pcrmentioning

confidence: 99%

Cloning, characterization and expression analysis of caspase-8 genes from the golden pompano (Trachinotus ovatus)

Wan

Lin

et al. 2022

Front. Mar. Sci.

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Bacterial infection triggers apoptosis and immunity by regulating the expression of caspases. In this article, we report two caspase-8 paralogs, termed ToCas8 and ToCas8-like, from an economically important teleost golden pompano (Trachinotus ovatus). Caspase-8 is a classic cysteine protease that is essential for the initiation and execution of apoptosis and is a critical regulator in initiating death receptor-induced programmed cell death. The two proteins encoded by ToCas8 and ToCas8-like were characterized by multiple sequence alignment and protein structure analysis showing similarity to their counterparts in vertebrates and exhibiting typical features of the Caspase-8 protein family. Gene synteny analysis further confirmed that two isoforms of caspase-8 genes exactly existed in golden pompano. Phylogenetic analysis showed that the Caspase-8 proteins of the selected species in the study were clustered together, and Cas8 and Cas8-like were clustered into two different subgroups. In addition, ToCas8 and ToCas8-like were widely distributed and both were expressed at high levels in gill, kidney and intestinal tissues. Transcript levels of ToCas8 and ToCas8-like were significantly upregulated in response to LPS and Poly(I:C) challenges, suggesting that caspase-8 genes are involved in the immune response to pathogens. In summary, we have identified two isoforms of caspase-8 in golden pompano that are involved in the immune response. These findings will expand our understanding of the evolutionary history and functional characteristics of caspase-8 genes in vertebrates.

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Molecular characterization, expression and response to immune challenges of 3 members of the toll-like receptor superfamily 11 in the golden pompano (Trachinotus ovatus)

Cited by 2 publications

References 28 publications

Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity

Characterization of the toll‐like receptor 13 homologs in American bullfrogs, Lithobates catesbeianus, and their involvement in antimicrobial immunity

Cloning, characterization and expression analysis of caspase-8 genes from the golden pompano (Trachinotus ovatus)

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