Molecular characterization, expression and functional analysis of NOD1, NOD2 and NLRC3 in Nile tilapia ( Oreochromis niloticus )

Gao, Fengying; Pang, Ji-cai; Lu, Maixin; Yang, Xianle; Zhu, Hai‐Lei; Ke, Xiaoli; Liu, Zhigang; Cao, Jianhua; Wang, Miao

doi:10.1016/j.fsi.2017.12.012

Cited by 52 publications

(26 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), Japanese flounder ( Paralichthys olivaceus ) (Li, Chen, Hao, Geng, Zhan et al. ), blunt snout bream ( Megalobrama amblycephala ) (Zhou et al., ), mrigal ( Cirrhinus mrigala ) (Swain, Basu, & Samanta, ), Catla ( Catla catla ) (Swain, Maiti, & Samanta, ), Nile tilapia ( Oreochromis niloticus ) (Gao et al., ) and rohu ( Labeo rohita ) (Swain, Basu, Sahoo, Maiti, Routray et al. ; Swain, Basu, & Samanta, ).…”

Section: Classification and Structure Of Nlr Familymentioning

confidence: 99%

“…NOD1 and NOD2 in goldfish ( Carassius auratus L .) (Xie et al., ), grass carp ( Ctenopharyngodon idella ) (Chen, Xu, Chang, Nie et al., ), mrigal ( Cirrhinus mrigala ) (Swain, Basu, & Samanta, ), orange‐spotted grouper ( Epinephelus coioides ) (Hou et al., ), and Nile tilapia ( Oreochromis niloticus ) (Gao et al., ) and NOD1 in catla ( Catla catla ) (Swain, Maiti, & Samanta, ) have also been identified and characterized. The phylogenetic tree analysis showed that grouper ( Epinephelus coioides ) NOD1 clustered with grass carp ( Ctenopharyngodon idella ), zebrafish ( Danio rerio ) and channel catfish ( Ictalurus punctatus ), whereas NOD2 was most closely related to Japanese pufferfish ( Takifugu rubripes ).…”

Section: Identification Of Nlrs and Splice Variants In Teleost Fishmentioning

confidence: 99%

“…Analysis of the genome database led to the identification of 13 NLR genes in Japanese pufferfish ( Takifugu rubripes ) that were named NLR‐C1‐13, based upon phylogenetic similarities (Biswas et al., ). In Asian seabass ( Lates calcarifer ) (Paria et al., ), blunt snout bream ( Megalobrama amblycephala ) (Zhou et al., ), and Nile tilapia ( Oreochromis niloticus ) (Gao et al., ), NLR‐C3 gene was cloned and characterized. Using the tBLASTn program, analysis of the turbot transcriptome database revealed the presence of two NLR‐C3 splice variants, NLR‐C3a and NLR‐C3b (Gao et al., ; Hou et al., ).…”

Section: Identification Of Nlrs and Splice Variants In Teleost Fishmentioning

confidence: 99%

“…The highest expression levels of the two NLRs were in the spleen. In addition, after injection of Streptococcus agalactiae , expression levels of NOD1 and NOD2 were obviously changed in the blood, spleen, kidney, intestine and gill (Gao et al., ). In the untreated mrigal ( Cirrhinus mrigala ), the highest expression of NOD1 and NOD2 was detected in liver and blood, respectively.…”

Section: Expression Of Nlrs In Teleost Fishmentioning

confidence: 99%

“…Similarly, in Asian seabass ( Lates calcarifer ), the basal expression of NLR‐C3 is highest in the gill and intestine, with modest expression levels also found in the liver (Paria et al., ). Nile tilapia ( Oreochromis niloticus ) NLRC3 gene was extensively expressed in the tissues as NOD1 and NOD2, and the expression was significantly up‐regulated after the infection of Streptococcus agalactiae , especially in the gill and blood (Gao et al., ). In Miiuy croaker ( Miichthys miiuy ), the basal expression of NLR‐C3 is also highest in the gill, intestine and blood (Li et al., ), suggesting that in at least three species, expression of NLR‐C genes is highest in tissues important for immune function.…”

Section: Expression Of Nlrs In Teleost Fishmentioning

confidence: 99%

See 4 more Smart Citations

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Zhang

Gao

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

Nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) are a group of intracellular pathogen recognition receptors (PRRs) that play key roles in pathogen recognition and subsequent activation of innate immune signalling pathways. Expressions of several NLR subfamily members, including NOD1, NOD2, NLR-C3, NLR-C5 and NLR-X1 have been reported in many different teleost fish species. These receptors are activated by a variety of ligands, including lipopolysaccharides (LPS), peptidoglycans (PGN) and polyinosinic-polycytidylic acid [Poly(I:C)]. Synthetic dsRNA and bacterial or viral infections are known to stimulate these receptors both in vitro and in vivo. In this review, we focus on the identification, expression and function of teleost NLRs in response to bacterial or viral pathogens. Additionally, NLR ligand specificity and signalling pathways involved in the recognition of bacterial or viral stimuli are also summarized. This review focuses on current knowledge in this area and provides future perspectives regarding topics in need of additional investigation. Understanding the response of innate immune system to bacterial or viral infections in diverse species could inform the development of more effective therapies and vaccines.

show abstract

Section: Classification and Structure Of Nlr Familymentioning

confidence: 99%

Section: Identification Of Nlrs and Splice Variants In Teleost Fishmentioning

confidence: 99%

Section: Identification Of Nlrs and Splice Variants In Teleost Fishmentioning

confidence: 99%

Section: Expression Of Nlrs In Teleost Fishmentioning

confidence: 99%

Section: Expression Of Nlrs In Teleost Fishmentioning

confidence: 99%

See 3 more Smart Citations

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Zhang

Gao

et al. 2018

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

Bakry,

Emeish,

Embark

et al. 2024

J Aqua Anim Hlth

View full text Add to dashboard Cite

ObjectiveDuring Egypt's hot summer season, Aeromonas veronii infection causes catastrophic mortality on Nile Tilapia Oreochromis niloticus farms. Egypt is ranked first in aquaculture production in Africa, sixth in aquaculture production worldwide, and third in global tilapia production. This study aimed to investigate, at the molecular level, the early innate immune responses of Nile Tilapia to experimental A. veronii infection.MethodsThe relative gene expression, co‐expression clustering, and correlation of four selected immune genes were studied by quantitative real‐time polymerase chain reaction in four organs (spleen, liver, gills, and intestine) for up to 72 h after a waterborne A. veronii challenge. The four genes studied were nucleotide‐binding oligomerization domain 1 (NOD1), lipopolysaccharide‐binding protein (LBP), natural killer‐lysin (NKL), and interleukin‐1 beta (IL‐1β).ResultThe four genes showed significant transcriptional upregulation in response to infection. At 72 h postchallenge, the highest NOD1 and IL‐1β expression levels were recorded in the spleen, whereas the highest LBP and NKL expression levels were found in the gills. Pairwise distances of the data points and the hierarchical relationship showed that NOD1 clustered with IL‐1β, whereas LBP clustered with NKL; both genes within each cluster showed a significant positive expression correlation. Tissue clustering indicated that the responses of only the gill and intestine exhibited a significant positive correlation.ConclusionThe results suggest that NOD1, LBP, NKL, and IL‐1β genes play pivotal roles in the early innate immune response of Nile Tilapia to A. veronii infection, and the postinfection expression profile trends of these genes imply tissue‐/organ‐specific responses and synchronized co‐regulation.

show abstract

In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

Dinakaran,

Prasad,

Bedekar

et al. 2024

Arch Virol

View full text Add to dashboard Cite

Molecular characterization, expression and functional analysis of NOD1, NOD2 and NLRC3 in Nile tilapia ( Oreochromis niloticus )

Cited by 52 publications

References 55 publications

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Nucleotide‐binding and oligomerization domain (NOD)‐like receptors in teleost fish: Current knowledge and future perspectives

Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

In vitro analysis of the expression of inflammasome, antiviral, and immune genes in an Oreochromis niloticus liver cell line following stimulation with bacterial ligands and infection with tilapia lake virus

Contact Info

Product

Resources

About