Molecular characterization, expression analysis, and ontogeny of complement component C9 in southern catfish (Silurus meridionalis)

Fu, Yao-Wu; Zhu, Changlian; Zhang, Qizhong; Hou, Ting-Long

doi:10.1016/j.fsi.2018.11.069

Cited by 11 publications

(16 citation statements)

References 33 publications

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“…TSP1 (thrombodopsin 1) protein is an adhesive glycoprotein that mediates cell‐to‐cell and cell‐to‐matrix interactions. The presence of a second TSP1 domain in C‐terminal region of C9 protein is the characteristics of primitive carp complement system, which is reported in several teleosts (Japanese flounder, rainbow trout, trout, southern catfish and grass crap), but absent in Chiloscyllium plagiosum , mammalian and birds (Fu et al, ; Meng et al, ). EGF (epidermal growth factor) helps in cellular proliferation, differentiation and survival of a cell.…”

Section: Discussionmentioning

confidence: 99%

“…Rohu C9 possesses a coding region of 666 amino acids with a signal peptide of 19 amino acids as observed in the present study. Similarly, C9 gene includes 593 amino acids in rock bream (Wickramaarachchi et al, ), 650 amino acids in grass carp (Li et al, ), 602 amino acids in southern catfish (Fu et al, ) and 694 amino acids in largemouth bronze gudgeon (Fu et al, ). However, a small C9 protein of 461 amino acids was observed in miiuy crocker (Meng et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Japanese flounder ( Paralichthys olivaceus ) (Katagiri, Hirono, & Aoki, ), puffer fish ( Fugu rubripes ) (Yeo, Elgar, Sandford, & Brenner, ), carp ( Cyprinus carpio ) (Uemura, Yano, Shiraishi, & Nakao, ), rainbow trout ( Oncorhynchus mykiss ) (Tomlinson, Stanley, & Esser, ), grass carp ( Ctenopharyngodon idella ) (Li, Chang, & Nie, ), largemouth bronze gudgeon ( Coreius guichenoti ) (Fu, Zhu, & Zhang, ) and southern catfish ( Silurus meridionalis ) (Fu, Zhu, Zhang, & Hou, ). The liver seems to be the primary site of synthesis for C9 with most abundant expression (Fu et al, ; Li et al, ; Wang, Xu, Yuhao, Ye, & Hua, ; Wickramaarachchi et al, ). However, the expression of this gene during the developmental stages has not yet been studied in any carps except recently looked into in southern catfish (Fu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The liver seems to be the primary site of synthesis for C9 with most abundant expression (Fu et al, ; Li et al, ; Wang, Xu, Yuhao, Ye, & Hua, ; Wickramaarachchi et al, ). However, the expression of this gene during the developmental stages has not yet been studied in any carps except recently looked into in southern catfish (Fu et al, ). Its expression has been noticed to be up‐regulated during bacterial and viral infections in fish.…”

Section: Introductionmentioning

confidence: 99%

“…Its expression has been noticed to be up‐regulated during bacterial and viral infections in fish. C9 was reported to be potentially induced by infection with Edwardsiella tarda , Streptococcus iniae , Aeromonas hydrophila , LPS and iridovirus in rock bream, indicating its important role in immune defence (Fu et al, ; Wickramaarachchi et al, ). C9 transcripts were found to be raised in fins, liver, spleen and head kidney at 48 hr post challenge with viral haemorrhagic septicaemia virus (VHSV) infection in zebrafish (Encinas et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and induced expression analysis of the terminal complement component C9 in rohu,Labeo rohita

et al. 2019

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The complement component 9 (C9) plays a significant role in the formation of membrane attack complex (MAC) on the targeted cell surface. The current study is dealt with molecular characterization of C9 gene from rohu, Labeo rohita, an important cultured carp species in India. An open reading frame (ORF) of 1998 bp was amplified by polymerase chain reaction (PCR) that encodes a polypeptide of 666 amino acids having a signal peptide of 19 amino acids and a mature peptide of 647 amino acids. The SMART domain architecture analysis revealed two thrombospondin type‐1 domains (TSP1), a low‐density lipoprotein receptor domain class A (LDLa), a membrane attack complex and perforin (MACPF) domain, and an epidermal growth factor (EGF)‐like domain. Multiple sequence alignment and evolutionary analysis revealed a primitive C9 sequence of rohu with maximum similarity and clustering with common carp, grass carp and zebrafish. C9 was highly expressed in liver and constitutively expressed in wide array of tissues except in eye of rohu juveniles. It was expressed during early developmental days of rohu including in milt. A variable level of up‐regulation in C9 expression was noticed upon poly I:C induction, Aeromonas hydrophila and Argulus siamensis infections in liver, spleen and gill tissues of rohu at different time points. A constitutive expression of C9 in different stages of rohu during the ontogeny and in response to pathogen exposures along with high degree of sequence homology with other fish species proved it as an important primitive immune molecule of the complement system lytic pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and induced expression analysis of the terminal complement component C9 in rohu,Labeo rohita

et al. 2019

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Innate Immunity

Dalmo

Bøgwald

2022

Principles of Fish Immunology

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Research on the innate immunity has accelerated over the last decades. The main reason for this has been the discovery of receptors recognizing danger molecules from pathogens. This has been facilitated through genome and transcriptome sequencing of different fish species. Also, endogenous host molecules from sterile physiological insults may also bind to certain receptors and induce immunological processes. The magnitude and quality of adaptive immunity are known to be dependent on the instructions the innate response gives. This chapter gives an overview of selected innate immune organs/tissues, factors, and processes that have been suggested to possess important roles during innate immune response in fish.

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Transcriptomic assessment of dietary fishmeal partial replacement by soybean meal and prebiotics inclusion in the liver of juvenile Pacific yellowtail (Seriola lalandi)

et al. 2021

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Molecular characterization, expression analysis, and ontogeny of complement component C9 in southern catfish (Silurus meridionalis)

Cited by 11 publications

References 33 publications

Molecular characterization and induced expression analysis of the terminal complement component C9 in rohu,Labeo rohita

Molecular characterization and induced expression analysis of the terminal complement component C9 in rohu,Labeo rohita

Innate Immunity

Transcriptomic assessment of dietary fishmeal partial replacement by soybean meal and prebiotics inclusion in the liver of juvenile Pacific yellowtail (Seriola lalandi)

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