Molecular characterization, expression analysis and association study with immune traits of porcine PSMB6 gene

Wu, Ximei; Wang, Yanfang; Sun, Yunzi

doi:10.1007/s11033-011-0866-3

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…OLE can partly protect against HI-induced molecular and cellular pathology in piglet white matter [6,23]. Other than a few molecular cloning studies of swine proteasome subunit genes [69][70][71][72], there is scant information available on swine proteasomes. Little work has been done to characterize newborn piglet proteasomes with the exception of P20S immunolocalization in brain [6].…”

Section: Discussionmentioning

confidence: 99%

Oleuropein Activates Neonatal Neocortical Proteasomes, but Proteasome Gene Targeting by AAV9 Is Variable in a Clinically Relevant Piglet Model of Brain Hypoxia-Ischemia and Hypothermia

Demerdash

Chen

O’Brien

et al. 2021

Cells

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Cerebral hypoxia-ischemia (HI) compromises the proteasome in a clinically relevant neonatal piglet model. Protecting and activating proteasomes could be an adjunct therapy to hypothermia. We investigated whether chymotrypsin-like proteasome activity differs regionally and developmentally in the neonatal brain. We also tested whether neonatal brain proteasomes can be modulated by oleuropein, an experimental pleiotropic neuroprotective drug, or by targeting a proteasome subunit gene using recombinant adeno-associated virus-9 (AAV). During post-HI hypothermia, we treated piglets with oleuropein, used AAV-short hairpin RNA (shRNA) to knock down proteasome activator 28γ (PA28γ), or enforced PA28γ using AAV-PA28γ with green fluorescent protein (GFP). Neonatal neocortex and subcortical white matter had greater proteasome activity than did liver and kidney. Neonatal white matter had higher proteasome activity than did juvenile white matter. Lower arterial pH 1 h after HI correlated with greater subsequent cortical proteasome activity. With increasing brain homogenate protein input into the assay, the initial proteasome activity increased only among shams, whereas HI increased total kinetic proteasome activity. OLE increased the initial neocortical proteasome activity after hypothermia. AAV drove GFP expression, and white matter PA28γ levels correlated with proteasome activity and subunit levels. However, AAV proteasome modulation varied. Thus, neonatal neocortical proteasomes can be pharmacologically activated. HI slows the initial proteasome performance, but then augments ongoing catalytic activity. AAV-mediated genetic manipulation in the piglet brain holds promise, though proteasome gene targeting requires further development.

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Section: Discussionmentioning

confidence: 99%

Oleuropein Activates Neonatal Neocortical Proteasomes, but Proteasome Gene Targeting by AAV9 Is Variable in a Clinically Relevant Piglet Model of Brain Hypoxia-Ischemia and Hypothermia

Demerdash

Chen

O’Brien

et al. 2021

Cells

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“…The 20S proteasome is responsible for the breakdown of shortlived proteins involved in cellular apoptosis, DNA repair, endocytosis, cell cycle regulation and for the rapid removal of misfolded proteins [30]. PSMB6 belongs to the 20S proteasomal subunit family, which participates in catalyzing ubiquitin-protein degradation [31].…”

Section: Introductionmentioning

confidence: 99%

Identification of Proteasome Subunit Beta Type 6 (PSMB6) Associated with Deltamethrin Resistance in Mosquitoes by Proteomic and Bioassay Analyses

Sun

et al. 2013

PLoS ONE

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Deltamethrin (DM) insecticides are currently being promoted worldwide for mosquito control, because of the high efficacy, low mammalian toxicity and less environmental impact. Widespread and improper use of insecticides induced resistance, which has become a major obstacle for the insect-borne disease management. Resistance development is a complex and dynamic process involving many genes. To better understand the possible molecular mechanisms involved in DM resistance, a proteomic approach was employed for screening of differentially expressed proteins in DM-susceptible and -resistant mosquito cells. Twenty-seven differentially expressed proteins were identified by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Four members of the ubiquitin-proteasome system were significantly elevated in DM-resistant cells, suggesting that the ubiquitin-proteasome pathway may play an important role in DM resistance. Proteasome subunit beta type 6 (PSMB6) is a member of 20S proteasomal subunit family, which forms the proteolytic core of 26S proteasome. We used pharmaceutical inhibitor and molecular approaches to study the contributions of PSMB6 in DM resistance: the proteasome inhibitor MG-132 and bortezomib were used to suppress the proteasomal activity and siRNA was designed to block the function of PSMB6. The results revealed that both MG-132 and bortezomib increased the susceptibility in DM-resistant cells and resistance larvae. Moreover, PSMB6 knockdown decreased cellular viability under DM treatment. Taken together, our study indicated that PSMB6 is associated with DM resistance in mosquitoes and that proteasome inhibitors such as MG-132 or bortezomib are suitable for use as a DM synergist for vector control.

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“…Immunological traits g.G443A [67] TAP1 PRRSV resistance c.933A > G [68] TLR3 PRRSV susceptibility c.761A > G [69] IRF7 Health and immunity g.2115T > C [70] LMP2 Haematological traits g.1232C > G [70] LMP7 Haematological traits c.C522T [71] BPI Disease resistance c.A1060G [71] BPI Disease resistance c.C1027A [72] TLR4 Salmonella shedding c.8C > G [73] PSMB6 Immunological traits c.144T > C [74] BCL10 Immunological traits [75][76][77] . Although CRISPR/Cas9 is used extensively to make precise insertions, deletions or any point mutation of interest, a number of limitations are attributed to HDR editing.…”

Section: Snps Responsible For Disease Resistancementioning

confidence: 99%

Base editing in pigs for precision breeding

Song¹,

Wang²,

Zhao³

2020

Front. Agr. Sci. Eng.

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Pigs are one of the most important domesticated animals and have great value in agriculture and biomedicine. Single nucleotide polymorphisms (SNPs) are a dominant type of genetic variation among individual pigs and contribute to the formation of traits. Precision single base substitution provides a strategy for accurate genetic improvement in pig production with the characterization of functional SNPs and genetic variants in pigs. Base editing has recently been developed as the latest gene-editing tool that can directly make changes in single nucleotides without introducing double-stranded DNA breaks (DSBs), providing a promising solution for precise genetic modification in large animals. This review summarizes gene-editing developments and highlights recent genetic dissection related to SNPs in major economic traits which may have the potential to be modified using SNP-editing applications. In addition, limitations and future directions of base editing in pig breeding are discussed.

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Molecular characterization, expression analysis and association study with immune traits of porcine PSMB6 gene

Cited by 4 publications

References 19 publications

Oleuropein Activates Neonatal Neocortical Proteasomes, but Proteasome Gene Targeting by AAV9 Is Variable in a Clinically Relevant Piglet Model of Brain Hypoxia-Ischemia and Hypothermia

Oleuropein Activates Neonatal Neocortical Proteasomes, but Proteasome Gene Targeting by AAV9 Is Variable in a Clinically Relevant Piglet Model of Brain Hypoxia-Ischemia and Hypothermia

Identification of Proteasome Subunit Beta Type 6 (PSMB6) Associated with Deltamethrin Resistance in Mosquitoes by Proteomic and Bioassay Analyses

Base editing in pigs for precision breeding

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