Molecular characterization, dynamic transcription, and potential function of KIF3A/KIF3B during spermiogenesis in Opsariichthys bidens

Lin, Chenwen; Tang, Daojun; Gao, Xinming; Jiang, Huayu; Du, Chang; Zhu, Junquan

doi:10.1016/j.gene.2021.145795

Cited by 12 publications

(5 citation statements)

References 57 publications

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“…The Opsariichthys bidens (Cyprinidae) is a small economic fish widely distributed in mountain streams, lakes and rivers across East Asia, due to its delicious taste and high nutritional value, with widely cultured in the southeast of China (Lin et al, 2021). For a long time, this species has been considered a carnivorous fish because a large number of insect limbs have been found in the intestines of wild individuals.…”

Section: Introductionmentioning

confidence: 99%

Effects of fishmeal replacement with eight protein sources on growth performance, blood biochemistry and stress resistance in Opsariichthys bidens

et al. 2021

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Nine experimental diets were formulated to study the effects of fishmeal replacement on Opsariichthys bidens. Weight gain rate, protein efficiency rate and apparent digestibility coefficient of fish in fishmeal (FM), maggot meal (MM) and soybean meal (SM) groups were significantly the highest. There was no significant difference in intestinal amylase activity between FM and SM groups. Principal component analysis showed that SM group and wild fish (WF) individual had similar essential amino acid compositions in muscle. The lowest value of serum malondialdehyde and the highest value of total antioxidant capacity were found in SM group, but fish in MM group did the opposite. Fish in MM group had the higher whole-body lipid, serum triglycerides, glucose, complement C3, C4 and immunoglobulin contents and lysozyme activity. After ammonia exposure, cumulative mortality of fish in FM and SM groups was significantly the lowest. Liver carbamyl phosphate synthetase activity of fish in FM group was significantly the highest, but the highest values of glutamine synthetase and glutamate dehydrogenase were found in SM group. This study suggests that fishmeal replacement with soybean meal is acceptable, and muscle amino acid compositions of fish in SM group were more similar to wild individual.

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Section: Introductionmentioning

confidence: 99%

Effects of fishmeal replacement with eight protein sources on growth performance, blood biochemistry and stress resistance in Opsariichthys bidens

et al. 2021

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“… 24 The combination of KIF3A and KIF3B is essential for forward transportation in the cytoplasm, especially during spemiogenesis. 25 The combination of KIF3A and KIF3C is responsible for particle transportation of axons and dendrites of nerve cells. 26 KIF3A knockout causes the loss of renal cilia, leading to polycystic kidney disease (PKD).…”

Section: Kif3a In Kinesin Familymentioning

confidence: 99%

Kinesin family member 3A induces related diseases via wingless-related integration site/β-catenin signaling pathway

Liu

et al. 2023

Science Progress

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Kinesin family member 3A is an important motor protein that participates in various physiological and pathological processes, including normal tissue development, homeostasis maintenance, tumor infiltration, and migration. The wingless-related integration site/β-catenin signaling pathway is essential for critical molecular mechanisms such as embryonic development, organogenesis, tissue regeneration, and carcinogenesis. Recent studies have examined the molecular mechanisms of kinesin family member 3A, among which the wingless-related integration site/β-catenin signaling pathway has gained attention. The interaction between kinesin family member 3A and the wingless-related integration site/β-catenin signaling pathway is compact and complex but is fascinating and worthy of further study. The upregulation and downregulation of kinesin family member 3A influence many diseases and patient survival through the wingless-related integration site/β-catenin signaling pathway. Therefore, this review mainly focuses on describing the kinesin family member 3A and wingless-related integration site/β-catenin signaling pathways and their associated diseases.

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“…Then, cDNA was synthetized using a PrimeScript®RT Reagent Kit (Takara, Beijing, China) for qPCR. We performed qPCR to evaluate phb mRNA expression levels in all of the tissues examined and stage II-V testes of O. bidens as described by Lin et al [57]. According to the cloned full-length Ob-phb cDNA, specific primers and primers for β-actin (internal reference) were designed with the conditions of 80-150 bp between primers, 17-25 bp primer lengths, and 45-55% GC (Supplementary file).…”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

Wang

Gao

et al. 2022

IJMS

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Spermatogenesis is the intricate and coordinated process by which spermatogonia develop into haploid differentiated spermatozoa. Mitochondria are essential for spermatogenesis, and prohibitin (PHB) is closely associated with mitochondrial structure and function during spermatogenesis. Although PHB has been implicated in spermatogenesis in some taxa, its roles in Opsariichthys bidens have not been determined. In this study, the expression patterns and potential functions of PHB in spermatogenesis in O. bidens were characterized using histological microscopic observations, PCR cloning, real-time quantitative PCR (qPCR), Western blotting (WB) and immunofluorescence (IF). The full-length cDNA of Ob-phb was 1500 bp encoding 271 amino acids. A sequence alignment demonstrated that the PHB protein is conserved among different animals. qPCR revealed that phb mRNA is widely distributed in O. bidens and highly expressed in the testes at stages IV and V. WB revealed that Ob-PHB is located in the mitochondria of testes. IF revealed the colocalization of PHB signals and mitochondria. Signals were detected around nuclei in spermatogonia and spermatocytes, gradually moving to the tail region during spermiogenesis, and finally aggregating in the midpiece. These results indicate that Ob-PHB was expressed in the mitochondria during spermatogenesis. In addition, this study proposed Ob-PHB may participate in the degradation of mitochondria and cell differentiation during spermatogenesis.

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Molecular characterization, dynamic transcription, and potential function of KIF3A/KIF3B during spermiogenesis in Opsariichthys bidens

Cited by 12 publications

References 57 publications

Effects of fishmeal replacement with eight protein sources on growth performance, blood biochemistry and stress resistance in Opsariichthys bidens

Effects of fishmeal replacement with eight protein sources on growth performance, blood biochemistry and stress resistance in Opsariichthys bidens

Kinesin family member 3A induces related diseases via wingless-related integration site/β-catenin signaling pathway

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

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