Molecular characterization, constitutive expression and GTP binding mechanism of Cirrhinus mrigala (Hamilton, 1822) Myxovirus resistance (Mx) protein

Roy, Pragyan; Rout, Ajaya Kumar; Maharana, Jitendra; Sahoo, Deepak Ranjan; Panda, Soumya Prasad; Pal, Arttatrana; Nayak, Kausalya Kumari; Behera, Bijay Kumar; Das, Basanta Kumar

doi:10.1016/j.ijbiomac.2019.06.161

Cited by 12 publications

(7 citation statements)

References 90 publications

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“…Mx is not only an antiviral interferon-inducible gene but also induced by several biotic and abiotic stressors including disease pathogenesis. The induction of Mx gene expression by poly I: C in L. rohita and C. mrigala was recently reported (Das et al, 2019;Roy et al, 2019). In this study, the L. catla Mx gene expression after stimulation with poly I:C is reported.…”

Section: Introductionsupporting

confidence: 51%

“…of healthy catla fishes were selected for the experiment and were distributed into two groups of 28 nos. each, out of which fishes of one group (treatment group) were intraperitoneally injected with 0.1 ml Poly I:C solution, as prescribed previously (Das et al, 2019;Roy et al, 2019). The second set of 28 fishes were maintained as control group.…”

Section: Poly I: C Treatment and Blood Sample Collectionmentioning

confidence: 99%

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Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

Panda¹,

Soren²,

Nayak³

et al. 2021

Indian Journal of Fisheries

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In the current investigation, the kinetics of Mx transcripts expression in the blood of Labeo catla (Hamilton 1822) inoculated with poly I: C, was studied for 14 days. The level of Mx transcript in the blood cells increased as compared to control fish inoculated with phosphate buffered saline. The level of Mx transcript was quantified along with semi-quantitative expression for β-actin. The level of Mx transcript was maximum on day 2 and 3 (β-actin: Mx ratio of about 0.8) which reduced after day 4 and the response disappeared on day 7. Western blots of the blood showed an immune-reactive band of 72 kDa, which is typical for L. catla Mx protein. Results suggest an increase in levels of Mx transcript after poly I: C injection which can stimulate an antiviral phase and the appropriateness of Mx expression investigation as an indicator to assess protective action.

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Section: Introductionsupporting

confidence: 51%

Section: Poly I: C Treatment and Blood Sample Collectionmentioning

confidence: 99%

Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

Panda¹,

Soren²,

Nayak³

et al. 2021

Indian Journal of Fisheries

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“…Comparative modeling's main benefit is that it aids in filling in gaps between available sequence and structural data by generating a reliable and accurate protein model [71]. The approach and parameters of MD simulation were adapted from our early studies to better comprehend the molecular function of unknown/hypothetical molecules [76][77][78][79][80][81].…”

Section: Discussionmentioning

confidence: 99%

Structural insights into the RNA interaction with Yam bean Mosaic virus (coat protein) from Pachyrhizus erosus using bioinformatics approach

et al. 2022

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Plants are constantly threatened by a virus infection, i.e., Potyviruses, the second largest genus of plant viruses which results in several million-dollar losses in various essential crops globally. Yam bean (Pachyrhizus erosus) is considered to be one of the essential tuberous legume crops holding a great potential source of starch. Yam Bean Mosaic Virus (YBMV) of Potyvirus group belonging to the family potyviridae affects Yam bean and several angiosperms both in the tropical and sub-tropical regions causing large economical losses in crops. In this study, we attempted to understand the sequence-structure relationship and mode of RNA binding mechanism in YBMV CP using in silico integrative modeling and all-atoms molecular dynamics (MD) simulations. The assembly of coat protein (CP) subunits from YBMV and the plausible mode of RNA binding were compared with the experimental structure of CP from Watermelon mosaic virus potyvirus (5ODV). The transmembrane helix region is present in the YBMV CP sequence ranging from 76 to 91 amino acids. Like the close structural-homolog, 24 CPs monomeric sub-units formed YBMV a conserved fold. Our computational study showed that ARG124, ARG155, and TYR151 orient towards the inner side of the virion, while, THR122, GLN125, SER92, ASP94 reside towards the outer side of the virion. Despite sharing very low sequence similarity with CPs from other plant viruses, the strongly conserved residues Ser, Arg, and Asp within the RNA binding pocket of YBMV CP indicate the presence of a highly conserved RNA binding site in CPs from different families. Using several bioinformatics tools and comprehensive analysis from MD simulation, our study has provided novel insights into the RNA binding mechanism in YBMV CP. Thus, we anticipate that our findings from this study will be useful for the development of new therapeutic agents against the pathogen, paving the way for researchers to better control this destructive plant virus.

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“…In 2015, in the cytosol of pea plumule cells ARF-like protein was discovered by Rout and Panigrahi. The amalgamation of immunological analysis and GTP-binding studies along with antibodies showed that the known Gα-subunits have identified potential heterotrimeric G protein subunits from barley (Hordeum vulgare) (Zbell et al, 1990), Arabidopsis, zucchini (Cucurbita pepo) (Roy et al, 2019), broad bean (Vicia faba) and Commelina communis (Zhou et al, 2009). There have been identified a 24 kDa GTP-binding protein in etiolated seedlings of oat (Avena sativa) by using western blot analysis using anti-G antibodies, GTP-binding assay and ADP ribosylation with cholera toxin.…”

Section: Detection Of Gtp-binding Proteinsmentioning

confidence: 99%

Era-like GTP protein gene expression in rice

et al. 2022

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The mutations are genetic changes in the genome sequences and have a significant role in biotechnology, genetics, and molecular biology even to find out the genome sequences of a cell DNA along with the viral RNA sequencing. The mutations are the alterations in DNA that may be natural or spontaneous and induced due to biochemical reactions or radiations which damage cell DNA. There is another cause of mutations which is known as transposons or jumping genes which can change their position in the genome during meiosis or DNA replication. The transposable elements can induce by self in the genome due to cellular and molecular mechanisms including hypermutation which caused the localization of transposable elements to move within the genome. The use of induced mutations for studying the mutagenesis in crop plants is very common as well as a promising method for screening crop plants with new and enhanced traits for the improvement of yield and production. The utilization of insertional mutations through transposons or jumping genes usually generates stable mutant alleles which are mostly tagged for the presence or absence of jumping genes or transposable elements. The transposable elements may be used for the identification of mutated genes in crop plants and even for the stable insertion of transposable elements in mutated crop plants. The guanine nucleotide-binding (GTP) proteins have an important role in inducing tolerance in rice plants to combat abiotic stress conditions.

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Molecular characterization, constitutive expression and GTP binding mechanism of Cirrhinus mrigala (Hamilton, 1822) Myxovirus resistance (Mx) protein

Cited by 12 publications

References 90 publications

Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

Poly I: C stimulates, Mx transcript and promotes an antiviral state in blood of Labeo catla (Hamilton 1822)

Structural insights into the RNA interaction with Yam bean Mosaic virus (coat protein) from Pachyrhizus erosus using bioinformatics approach

Era-like GTP protein gene expression in rice

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