Molecular Characterization and Viral Origin of the First Dengue Outbreak in Xishuangbanna, Yunnan Province, China, 2013

Guo, Xin; Yang, Henglin; Wu, Chao; Jiang, Jin-Yong; Fan, Jia; Li, Hongbin; Zhu, Jin; Yang, Zhonghua; Li, Yuanyuan; Zhou, Hongning; Zhang, Jiusong

doi:10.4269/ajtmh.14-0044

Cited by 26 publications

(35 citation statements)

References 9 publications

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“…3). The Laotian DENV-3 genotype II isolates were most closely related to those isolated from China (2013), Myanmar (2009), Bangladesh (2008), the Philippines (2010), Thailand (2010), Cambodia (2008), and Vietnam (2010) [25, 27]. All of the Laotian DENV-3 genotype II viruses obtained in this study and sequences from other Southeast Asian countries formed a monophyletic relationship with very high values bootstrap support (>98 %).…”

Section: Discussionmentioning

confidence: 95%

Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR

et al. 2016

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BackgroundDuring the 2013 outbreak, 4638 infection cases and 32 deaths have been recorded in the southern part of Laos. In recent years, the chikungunya virus (CHIKV) emerged in the part of the country bordering Cambodia. Dengue virus (DENV) and CHIKV are transmitted by common mosquito vectors. Both diseases have similar clinical presentations; therefore, CHIKV infections might go undiagnosed in DENV-endemic areas. Thus, rapid detection and accurate diagnosis are crucial for differentiating between the two viruses (DENV and CHIKV). In this study, we demonstrated that CHIKV and two serotypes of DENV are circulating in Laos. In addition, we encountered patients that had been concurrently infected with multiple DENV serotypes or DENV and CHIKV.MethodsPlasma samples were collected from 40 patients with suspected DENV infections during an outbreak between July and August 2013. The reverse transcription polymerase chain reaction was performed to detect the four DENV serotypes and CHIKV using specific primers. Specifically, the complete envelope gene sequences of the viruses were sequenced and subjected to phylogenetic analysis.ResultsForty acute-phase plasma samples from patients with suspected dengue infections were tested for the presence of DENV viral RNA using molecular methods. Among the 40 samples, 14 samples were positive for DENV, 2 samples were positive for both viruses (DENV-2 and DENV-3), whereas DENV-1 and DENV-4 were not detected during the study period. We also encountered 10 samples that were positive for CHIKV. Of the 10 CHIKV-positive samples, 3 samples were co-infected by DENV-2, and 2 samples were co-infected by DENV-3. Phylogenetic analysis revealed that the 2013 dengue outbreak in Laos involved DENV-2 genotype Asian I and DENV-3 genotype II. Moreover, the Laotian CHIKV strains grouped together with those isolated during outbreaks on the Indian Ocean Islands within the East Central South African genotype.ConclusionsThese findings revealed that two serotypes (DENV-2 and DENV-3) and CHIKV were detected. Furthermore, infection of multiple DENV serotypes and CHIKV was also observed in the 2013 dengue outbreak. This is the first documented evidence of co-infection with CHIKV and one of two DENV serotypes.

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Section: Discussionmentioning

confidence: 95%

Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR

et al. 2016

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“…Since the first outbreak of DF in Yunnan in 2008 [22], the number of DENV cases has been increasing in Xishuangbanna. There were 1319 cases confirmed DENV-3 in 2013, 1132 cases confirmed DENV-2 in 2015 and 1384 cases confirmed DENV-1 in 2017 [25,[29][30][31]. Therefore, it is important to continuously monitor the DF epidemic in Xishuangbanna.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of the viral structural protein genes of 2018 dengue virus serotype 1 epidemic in Yunnan, Southwest China

Lan

Yun²,

et al. 2020

Preprint

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Background There was a dengue virus serotype 1 (DENV-1) epidemic from October to December in 2018 in Xishuangbanna, Yunnan, southwest China, neighboring Myanmar, Laos and Vietnam. And the molecular characterization, evolution and potential source of DENV from Xishuangbanna were investigate. Methods C (capsid)/prM(premembrane)/E (envelope) genes of DNEV isolated from 87 serum samples obtained from local patients were amplified and sequenced, then evaluated using mutation, phylogenetic and homologous recombination analyses and secondary structure prediction. Results Phylogenetic analysis show that all the DENV strains from Xishuangbanna can grouped in two branch of DENV-1. Out of 52, 50 of isolates were grouped in branch one, and were most similar to Fujian (DQ193572) and Singapore 2016(MF314188) strains. The other two isolates have the closest relationship to Myanmar 2017 (MG679801) . When compared with DENV-1SS (standard strain), we found the amino acid residue of E155 was mutation from T to S,which may related to the virulence of virus and no obvious homologous recombination signals. Secondary structure prediction showed that some changes were found in the helical of MN123849 and MN123854 strains and few changes observed in disordered region. Conclusions This study revealed the molecular characterization of structural genes of xishuangbanna, 2018 epidemic strain, providing a reference for molecular epidemiology, infection, pathogenicity and vaccine development .

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“…Xishuangbanna is located in southwestern Yunnan Province, which is a border area of China, Burma and Laos. In 2013 and 2015, DENV-3 and DENV-2 outbreaks were reported in Xishuangbanna with 1319 and 1132 infections, respectively (Guo et al, 2015;Wang et al, 2016;Ren et al, 2015). In 2017, the first DENV-1 outbreak in the history of Xishuangbanna occurred.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of the viral structural gene of the first dengue virus type 1 outbreak in Xishuangbanna: A border area of China, Burma and Laos

Lin¹,

Ma²,

Wen³

et al. 2019

International Journal of Infectious Diseases

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Background: Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source and evolution of the viruses in outbreak. Methods: The structural protein C/prM/E genes of viruses isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock and demographic reconstruction analysis. Results: Phylogenetic analysis revealed that all of the strains were classified as three cluster of DENV-1. Cluster 1, 2 and 3 were most similar to China Guangzhou 2011, China Hubei 2014 and Laos 2008 strain, respectively. Among 236 base mutations, 31 caused nonsynonymous mutations when compared with the DENV-1SS. No homologous recombination signal was discovered. The structural protein of these strains had similar three-dimensional structure. Only site 434 showed differences among five predicted protein binding sites. Molecular clock phylogenetic and demographic reconstruction analysis showed that DENV-1 became highly diversified in 1972 followed by a slightly decreased period until 2017. Conclusions: Dengue isolated strains show diversification between Burma and China. Amino acid substitution (I440T) may lead to weakened virulence of the epidemic strains. DENV-1 became highly diversified in 1972 followed by a slightly decreased period.

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Molecular Characterization and Viral Origin of the First Dengue Outbreak in Xishuangbanna, Yunnan Province, China, 2013

Cited by 26 publications

References 9 publications

Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR

Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR

Molecular characterization of the viral structural protein genes of 2018 dengue virus serotype 1 epidemic in Yunnan, Southwest China

Molecular characterization of the viral structural gene of the first dengue virus type 1 outbreak in Xishuangbanna: A border area of China, Burma and Laos

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