Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

Hameed, Muhammad Salman; Zheng-bing, Wang; Vasseur, Liette; Guang, Yang

doi:10.3390/ijms19041249

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…The diamondback moth, Plutella xylostella , is a highly destructive pest of cruciferous plants worldwide. Nowadays, P. xylostella has become a model species in lepidopteran insects owing to its high fecundity rate and fast dispersal (Hameed et al ., ; Huang et al ., ; Wang et al ., ). The identification of sex‐, stage‐, tissue‐ and germline‐specific cis ‐regulatory elements provides a way for precise gene manipulation in lepidopteran insects.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of the cis‐regulatory region for the vitellogenin gene in Plutella xylostella

Asad

Munir

et al. 2020

Insect Molecular Biology

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The vitellogenin gene promoter (VgP) is an essential cis‐regulatory element that plays a significant role in transcription of the vitellogenin (Vg) gene, leading to the production of yolk protein in insects, including lepidopterans. However, the function of VgP is still not clear in Plutella xylostella. Here, we cloned a 5.1 kb DNA fragment of the cis‐regulatory region adjacent to the 5′ end of the Vg gene of P. xylostella (PxVg). We identified two promoter sites in that 5′ upstream sequence of PxVg and performed in vitro analysis of two promoter sequences (PxVgP1, 4.9 kb, and PxVgP2, 2.9 kb) in the embryonic cell line of P. xylostella. PxVgP2 exhibited higher enhanced green fluorescent protein (EGFP) expression, so PxVgP2 was used for in vivo analysis. Strong EGFP fluorescence was observed in adult females and the fat body of females, with low expression in embryos. Our results suggest that PxVgP is an important stage‐, tissue‐ and sex‐specific endogenous cis‐regulatory element in P. xylostella.

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Section: Introductionmentioning

confidence: 99%

Functional characterization of the cis‐regulatory region for the vitellogenin gene in Plutella xylostella

Asad

Munir

et al. 2020

Insect Molecular Biology

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“…Although RNAi was used for functional genetic research in insects of many orders, RNAi efficiency was reported to vary among species (Terenius et al ., 2011; Zhang et al ., 2017). Although successful RNAi effects have been reported in insects of many families of Lepidoptera such as Sphingidae, Noctuidae, Plutellidae, Tortricidae and Papilionidae (Choi and Vander, 2019; Christiaens et al ., 2018; Hameed et al ., 2018; Poreddy et al ., 2017; Wang et al ., 2012; Wang et al ., 2015), RNAi efficiency was reported to be relatively low in Lepidoptera, especially compared with the high RNAi sensitivity of Coleoptera and Diptera (Zhang et al ., 2017). Here we showed the effectiveness of transgene triggered RNAi effects on the Noctuidae insect M. separata , with HIGS effects comparable to those for other lepidopteran insects such as Manduca sexta and Helicoverpa armigera (Mao et al ., 2007; Poreddy et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

Improvement of host‐induced gene silencing efficiency via polycistronic‐tRNA‐amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata

Bao

Zhang

et al. 2021

Plant Biotechnology Journal

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Host-induced gene silencing (HIGS) emerged as a new strategy for pest control. However, RNAi efficiency is reported to be low in Lepidoptera, which are composed of many important crop pests. To address this, we generated transgenic plants to develop HIGS effects in a maize pest, Mythimna separata (Lepidoptera, Noctuidae), by targeting chitinase encoding genes. More importantly, we developed an artificial microRNA (amiR) based PTA (polycistronic-tRNA-amiR) system for silencing multiple target genes. Compared with hpRNA (hairpin RNA), transgenic expression of a PTA cassette including an amiR for the gut-specific dsRNA nuclease gene MsREase, resulted in improved knockdown efficiency and caused more pronounced developmental abnormalities in recipient insects. When target gene siRNAs were analysed after HIGS and direct dsRNA/siRNA feeding, common features such as sense polarity and siRNA hotspot regions were observed, however, they differed in siRNA transitivity and major 20-24nt siRNA species. Core RNAi genes were identified in M. separata, and biochemical activities of MsAGO2, MsSID1 and MsDcr2 were confirmed by EMSA (electrophoretic mobility shift assay) and dsRNA cleavage assays, respectively. Taken together, we provide compelling evidence for the existence of the RNAi mechanism in M. separata by analysis of both siRNA signatures and RNAi machinery components, and the PTA system could potentially be useful for future RNAi control of lepidopteran pests.

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“…Phylogenetic Tree and Sequence Analysis Ago protein sequences were downloaded from the GenBank database of NONCODE () and NCBI () (43). A multiple-sequence alignment was performed based on the PIWI domain using ClustalW software (version 2.1) (44).…”

Section: Methodsmentioning

confidence: 99%

LncRNA ROR1‑AS1 high expression and its prognostic significance in liver cancer

et al. 2019

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Hepatocellular carcinoma (HCC) is a common disease of the digestive system with no curative treatments. Long noncoding RNA tyrosine protein kinase transmembrane receptor 1 antisense RNA 1 (lncRNA ROR1-AS1) is an lncRNA whose functions have been predicted in human diseases; however, its important role in cancer has been probed only in mantle cell lymphoma, not in HCC. Therefore, the present study aimed to elucidate the prognostic significance of lncRNA ROR1-AS1 in HCC. The Cancer Genome Atlas Liver Hepatocellular Carcinoma was used to analyze the expression of ROR1-AS1 in liver cancer. χ 2 tests were performed to evaluate associations between clinical characteristics and ROR1-AS1 expression. The role of ROR1-AS1 in HCC prognosis was assessed using Kaplan-Meier curves and proportional hazards model (Cox) analysis. Gene set enrichment analysis was performed by using a Gene Expression Omnibus dataset. At the same time, Multi Experiment Matrix was used to predict genes that may be co-expressed with ROR1-AS1. The Database for Annotation, Visualization and Integrated Discovery and KO-Based Annotation System were used to analyze the most closely associated cytological behaviors and pathways in HCC. Then, the genes in the three databases were integrated to screen mRNAs, microRNAs and lncRNAs that had co-expression relationships with ROR1-AS1. Cytoscape, Search Tool for the Retrieval of Interacting Genes/Proteins and Molecular Evolutionary Genetics Analysis were used to map potential regulatory networks and developmental relationships associated with ROR1-AS1. Finally, 12 genes most closely associated with ROR1-AS1 were identified, and their relationship was described using a Circos plot. The results showed that ROR1-AS1 was upregulated in HCC, and its expression was related to clinical stage, T stage and N stage. Furthermore, Kaplan-Meier curves and Cox analysis indicated that high expression of ROR1-AS1 was associated with poor prognosis, and that ROR1-AS1 was an independent risk factor for HCC. Co-expression data suggested that there may be a large regulatory network of 45 genes with indirect associations with ROR1-AS1, a small regulatory network of 15 genes with direct or indirect regulatory relationships, and a special regulatory network containing 12 genes directly associated with ROR1-AS1. The present findings indicated that high expression of ROR1-AS1 suggests poor prognosis in patients with HCC.

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Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

Cited by 8 publications

References 43 publications

Functional characterization of the cis‐regulatory region for the vitellogenin gene in Plutella xylostella

Functional characterization of the cis‐regulatory region for the vitellogenin gene in Plutella xylostella

Improvement of host‐induced gene silencing efficiency via polycistronic‐tRNA‐amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata

LncRNA ROR1‑AS1 high expression and its prognostic significance in liver cancer

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