Molecular characterization and RNA interference responses of the lethal giant larvae gene in <i>Diabrotica virgifera virgifera</i> adults

Cooper, Anastasia M.W.; Song, Huifang; Shi, Xuekai; Yu, Zujiang; Kim, Young Ho; Silver, Kristopher; Zhang, Jianzhen; Zhu, Kun Yan

doi:10.1002/arch.21787

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…Lethal giant larvae seems to be encoded by a single copy gene in insects such as Ostrinia nubilalis , Diabrotica virgifera virgifera , Tribolium castaneum , and Drosophila melanogaster (Ohshiro et al ., 2000; Xiao et al ., 2014; Cooper et al ., 2021). Lgl was initially identified to be a tumor suppressor in Drosophila , of which l(2)gl mutant flies died at the larval stage and showed cancer‐like defects with uncontrolled growth of larval brain neuroblasts and imaginal discs (Ohshiro et al ., 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Silencing TcLgl by RNA interference (RNAi) in early larvae and late larvae resulted in 100% and 80% mortality, respectively. However, no phenotypes were observed when this gene was suppressed with RNAi in O. nubilalis , and suppression of DvLgl did not yield an observable phenotype in D. v. virgifera adults (Cooper et al ., 2021). Thus, the function of Lgl is still unclear in some insect species.…”

Section: Introductionmentioning

confidence: 99%

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Lethal giant larvae gene is required for normal nymphal development and midgut morphogenesis in Locusta migratoria

et al. 2022

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Disruption of morphogenesis, an essential process in organismal development, can lead to disruption of biological processes, reduction in fitness, or even death of an organism. The roles of lethal giant larvae (Lgl) protein in maintaining tissue organization have been studied extensively in mammals, but little is known about this gene's roles in promoting correct tissue morphogenesis in insects. In this study, we identified an Lgl ortholog in Locusta migratoria. RT‐qPCR results revealed that LmLgl was constitutively expressed during third, fourth, and fifth instar nymphs. Furthermore, LmLgl showed highest expression in the ovary followed by wing pads, midgut, hindgut, Malpighian tubules, and foregut of the third‐instar nymphs. To examine the role of LmLgl in L. migratoria development, RNA interference was performed during nymphal stages. Silencing of LmLgl increased body size but decreased bodyweight by 9.0%. Histological sections of the midgut revealed abnormal large masses of disordered epithelial cells in dsLmLgl‐injected nymphs. In addition, downregulation of LmLgl transcript levels significantly altered the morphological structure in midgut, resulting in the formation of tumor‐like structures. Our results indicated that LmLgl may act as a tumor‐suppressor gene, which plays an essential role in maintaining a normal morphological structure in the midgut of L. migratoria. Our results also suggest that LmLgl may be explored as a potential target for developing dsRNA‐based biological pesticides for managing insect pests.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lethal giant larvae gene is required for normal nymphal development and midgut morphogenesis in Locusta migratoria

et al. 2022

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Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

Hong,

Hu,

et al. 2024

Arch Insect Biochem Physiol

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Our previous research reported the influence of 50 μM selenium (Se) on the cytosolization (endocytosis) pathway, which in turn stimulates the growth and development of Bombyx mori. Lately, dynamin is recognized as one of the key proteins in endocytosis. To explore the underlying mechanisms of Se impact, the dynamin gene was knocked down by injecting siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3). This was followed by an analysis of the target gene and levels of silk protein genes, as well as growth and developmental indices, Se‐enrichment capacity, degree of oxidative damage, and antioxidant capacity of B. mori. Our findings showed a considerable decrease in the relative expression of the dynamin gene in all tissues 24 h after the interference and a dramatic decrease in the silkworm body after 48 h. RNAi dynamin gene decreased the silkworm body weight, cocoon shell weight, and the ratio of cocoon. In the meantime, malondialdehyde level increased and glutathione level and superoxide dismutase/catalase activities decreased. 50 μM Se markedly ameliorated these growth and physiological deficits as well as decreases in dynamin gene expression. On the other hand, there were no significant effects on fertility (including produced eggs and laid eggs) between the interference and Se treatments. Additionally, the Se content in the B. mori increased after the dynamin gene interference. The dynamin gene was highly expressed in the silk gland and declined significantly after interference. Among the three siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3), the dynamin‐2 displayed the highest interference effects to target gene expression. Our results demonstrated that 50 μM Se was effective to prevent any adverse effects caused by dynamin knockdown in silkworms. This provides practical implications for B. mori breeding industry.

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Development of dsRNA as a Sustainable Bioinsecticide: From Laboratory to Field

Rodrigues

Sridharan

Manley

et al. 2021

ACS Symposium Series

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Molecular characterization and RNA interference responses of the lethal giant larvae gene in Diabrotica virgifera virgifera adults

Cited by 3 publications

References 37 publications

Lethal giant larvae gene is required for normal nymphal development and midgut morphogenesis in Locusta migratoria

Lethal giant larvae gene is required for normal nymphal development and midgut morphogenesis in Locusta migratoria

Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

Development of dsRNA as a Sustainable Bioinsecticide: From Laboratory to Field

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