Molecular Characterization and Potential of Fungal Species Associated with Cassava Waste

Elijah, Aniekpeno Isaac; Asamudo, N. U.

doi:10.9734/bbj/2016/16559

Cited by 3 publications

(5 citation statements)

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“…Bakri et al (2009), Geetha et al, (2011), Fadahunsi and Garuba (2012) in their separate studies reported that Aspergillus flavus was the best producer of amylase among the fungal isolates screened for production of the enzyme. A recent study carried out by Elijah and Asamudo (2016) reported the presence of Aspergillus flavus among the fungal species isolated from cassava peel (CP) in a dumpsite using molecular characterization methods. Among the Aspergillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…The soil around garri processing sites are usually contaminated by spill or underground leakage of cassava waste water which are usually not treated by the local producers (Oboh, 2005;Kolawole, 2014). Nevertheless, exploiting microorganisms found in a garri processing site which is usually polluted for the production of microbial products is a research-driven effort with economic benefits (Elijah and Asamudo, 2016;Pham et al, 2019). Using traditional microbiological methods, Ogbonna et al (2014) identified fungal isolates namely Aspergillus niger, Penicillium sp.…”

Section: Introductionmentioning

confidence: 99%

“…from garri processing site with varying potentials to produce amylase. In a recent study, Elijah and Asamudo (2016) identified twenty-seven fungal species from cassava waste water using molecular methods and reported Saccharomyces cerevisiae (8.7 %) and Candida krusei (8.7 %) as the dominant species. According to Ayansina et al (2014), microorganisms capable of producing amylase could be found in soils around mills, cassava farms etc.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Ire

Wokoma

Okoli

2020

JLBSR

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Amylases (E.C.3.2.1.1) are enzymes which catalyze the breakdown of -1,4 glycosidic linkages of starch to simple sugars and different monomeric products. Microbial amylases are essential enzymes compared with animals and plants amylases in industrial applications. Thus, in southern Nigeria, garri processing waste-water from mills poses a serious environmental challenge and this situation could be exploited by utilizing microorganisms colonizing the area to manufacture microbial products. This present study was aimed at evaluating the potential of fungi isolated from garri processing environment for amylase secretion and conduct a time course study of the enzyme production. Fungi were isolated from the soil and waste water from garri processing sites using Standard Microbiological Procedures. The five fungi isolated from garri processing environmental samples were screened using Lugols iodine. Three fungal isolates were then selected on the hydrolysis of starch in qualitative zonation agar plates but one isolate named B among them was better in starch hydrolysis related to highest clear zone plates. After qualitative screening, the three hyper production amylase extracted fungi were identified according to the morphological characteristics. The hyper producing amylase isolate B was then identified as Aspergillus flavus (RCBBR_AEAFUN2) and recorded as a novel strain in southern Nigeria according to molecular characteristics, which was selected for further studies. The current results of time course study showed that the maximum growth (2.453 g) occurred after 24 h of incubation at pH 4.5 while maximum enzyme production (2.3 U/ml/min) was obtained after 96 h of incubation at pH 3.5. Therefore, the hyper producing amylase from garri processing sites, Aspergillus flavus (RCBBR_AEAFUN2) novel strain has great prospects for future biotechnological and industrial applications and help in ameliorating the environmental pollution posed by the waste-water.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Ire

Wokoma

Okoli

2020

JLBSR

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“…Cassava wastewater is the main effluent from the cassava press due to the industrial production of flour. The chemical composition basically constitutes carbohydrates, nitrogen, and several mineral salts, characterizing itself as a supplement with significant potential [25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

Maia

Santos

Fereira

et al. 2018

Colloids and Interfaces

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In this work, we investigated the potential of Bacillus subtilis UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE24) and the surface tension (ST). The ionic charge, stability (temperature, salinity, and pH measured by IE24 and viscosity), and ability to remove and disperse oil and textile dye were investigated. B. subtilis produced an anionic bioemulsifier in the medium containing 100% cassava wastewater under Condition 4 of the factorial design (inoculum 9% at a temperature of 35 °C and shaken at 100 rpm), and showed a surface tension of 39 mN/m, an IE24 of 95.2%, and a yield of 2.69 g·L−1. The bioemulsifier showed stability at different pH (2–8), temperatures (0–120 °C), and NaCl concentrations, a dispersion oil displacement area (ODA) test of 55.83 cm2, and a reduction of the viscosity of the burned engine oil (90.5 Cp). The bioemulsifier was able to remove petroleum (94.4%) and methylene blue azo dye (62.2%). The bioemulsifier and its synthesis from bacteria also emphasizes the role of surfactants in oil remediation.

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“…Thus, cassava wastewater is the main effluent from the cassava press due to the industrial production of flour. The chemical composition is basically constituted by carbohydrates, nitrogen and several mineral salts, characterizing itself as a supplement with significant potential [25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Producing-Bioemulsifier by <em>Bacillus Subtilis </em>UCP 0146 Isolated from Mangrove Sediments

Maia¹,

Santos²,

Ferreira³

et al. 2018

Preprint

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In this work was investigated the potential of Bacillus subtilis UCP 0146 in the bioconversion of the medium containing 100% of cassava flour wastewater to obtain bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE24) and surface tension (TS). The ionic charge, stability (temperature, salinity and pH measured by IE24 and viscosity), ability to remove and disperse oil and textile dye were investigated. B.subtilis produced an anionic bioemulsifier in the medium containing 100% of cassava wastewater in condition 4 of the factorial design (9% of the inoculum, at 35 °C and agitation of 100 rpm) with surface tension of 39mN/m, IE24 of 95.2 % and yield 2.69 g.L-1. Stability at different pH (2-8), temperatures (0-120ºC) and NaCl, dispersed (55.83 cm2-ODA) and reduced the viscosity of the burned engine oil (90.5 cP) , removed 94.4% petroleum and demonstrated efficiency in methylene blue removal (62.2%). The bioemulsifier and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation.

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Molecular Characterization and Potential of Fungal Species Associated with Cassava Waste

Cited by 3 publications

References 0 publications

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

An Efficient Producing-Bioemulsifier by <em>Bacillus Subtilis </em>UCP 0146 Isolated from Mangrove Sediments

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