Molecular Characterization and Physical Mapping of Two Classes of 5S rDNA in the Genomes of &lt;i&gt;Gymnotus sylvius&lt;/i&gt; and &lt;i&gt;G. inaequilabiatus&lt;/i&gt; (Gymnotiformes, Gymnotidae)

Scacchetti, Priscilla Cardim; Alves, José Carlos Pansonato; Utsunomia, Ricardo; Claro, Françoise; Toledo, Lurdes Foresti de Almeida; Oliveira, Cláudio; Foresti, Fausto

doi:10.1159/000335658

Cited by 15 publications

(17 citation statements)

References 49 publications

(23 reference statements)

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“…In the species in which it was mapped, the markings were species-specific, 4,33,42 which was also the case for the three species analyzed in this study.…”

Section: Discussionmentioning

confidence: 58%

New Approach Data in Electric Fish (Teleostei: Gymnotus): Sex Chromosome Evolution and Repetitive DNA

Silva

Matoso²,

Artoni³

et al. 2014

Zebrafish

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Antagonist sexual selection is the driving force behind the origin and diversification of sex chromosomes such as XX/XY and ZZ/ZW. However, chromosome mobility, mainly in fishes, may result in the formation of chromosomes of recent origin, a process known as turnover. The family Gymnotidae, which is composed of the genera Electrophorus+Gymnotus, presents a multiple system of the type X1X1X2X2/X1X2Y, which has been described for Gymnotus pantanal. This article describes the karyotype of three Amazon Gymnotus species, revealing the presence of both simple and multiple systems: Gymnotus carapo "Catalão" 2n=40 XX/XY, Gymnotus coropinae 2n=49♂/50♀ X1X1X2X2/X1X2Y, and Gymnotus sp. "Negro" 2n=50 XX/XY. Our hypothesis is that the simple system present in G. carapo "Catalão" is ancestral in relation to G. pantanal's multiple system and that the diversification of the subsequent multiple system occurred after the final separation of the Amazon and Paraná basins. Moreover, G. coropinae's multiple system may have originated from the simple system present in Gymnotus sp. "Negro." The distant position between the species in the Gymnotidae family's phylogeny in addition to differences in sex chromosome formula and number between Clade G1 G. coropinae and G. sp. "Negro" species and "Carapo" Clade. G. carapo and G. pantanal species suggest that both sequences of sexual systems occurred independently, supporting other proposed models and highlighting the fact that species of the genus Gymnotus may serve as a model for studying sex chromosome turnover.

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“…In the species in which it was mapped, the markings were species-specific, 4,33,42 which was also the case for the three species analyzed in this study.…”

Section: Discussionmentioning

confidence: 58%

New Approach Data in Electric Fish (Teleostei: Gymnotus): Sex Chromosome Evolution and Repetitive DNA

Silva

Matoso²,

Artoni³

et al. 2014

Zebrafish

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“…inaequilabiatus, G . pantherinus) have been studied previously [Scacchetti et al, 2011[Scacchetti et al, , 2012.…”

Section: Sampling and Preparation Of Mitotic Chromosomesmentioning

confidence: 99%

“…U2 snDNA probes corresponded to a 150-bp segment containing partial sequences of the U2 snRNA gene. The 5S rDNA probe included 120 bp of the 5S rRNA gene and 229 bp of the nontranscribed spacer [Scacchetti et al, 2012].…”

Section: Repetitive Probes and Fish Experimentsmentioning

confidence: 99%

Comparative Chromosome Mapping of U2 snRNA and 5S rRNA Genes in Gymnotus Species (Gymnotiformes, Gymnotidae): Evolutionary Dynamics and Sex Chromosome Linkage in G. pantanal

Utsunomia

Scacchetti

Pansonato-Alves

et al. 2014

Cytogenet Genome Res

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A comparative mapping of U2 small nuclear RNA (snRNA) and 5S ribosomal RNA (rRNA) genes was performed in 6 Gymnotus species. All species analyzed presented the U2 snDNA organized in conspicuous blocks and not co-located with rRNA genes. In addition, 5 species showed the U2 snDNA located in a single pair of chromosomes, which seems to be a conserved trait in this genus. Conversely, G. pantanal was the only species displaying several terminal signals in different chromosome pairs, including the X₁ sex chromosome but not the Y chromosome. This is the first report of U2 snRNA genes in sex chromosomes of fishes. The absence of sites in the Y chromosome of G. pantanal indicates a possible loss of terminal segments of the chromosomes involved in the Y formation.

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“…In neotropical fish, most comparative cytogenetic studies have focused on repetitive sequences of rRNA genes, which have shown that chromosome structures are very dynamic in relation to the number and location of rRNA clusters [Ferro et al, 2001;Kavalco et al, 2004;Scacchetti et al, 2012]. Otherwise, there is a great lack of studies for a detailed knowledge of the organization and distribution of histone genes within the fish genomes.…”

mentioning

confidence: 99%

Cytogenetic Mapping of H1 Histone and Ribosomal RNA Genes in Hybrids between Catfish Species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum

Hashimoto

Ferguson‐Smith²,

Rens³

et al. 2012

Cytogenet Genome Res

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A physical chromosome mapping of the H1 histone and 5S and 18S ribosomal RNA (rRNA) genes was performed in interspecific hybrids of Pseudoplatystoma corruscans and P. reticulatum. The results showed that 5S rRNA clusters were located in the terminal region of 2 chromosomes. H1 histone and 18S ribosomal genes were co-localized in the terminal portion of 2 chromosomes (distinct from the chromosomes bearing 5S clusters). These results represent the first report of association between H1 histone and 18S genes in fish genomes. The chromosome clustering of ribosomal and histone genes was already reported for different organisms and suggests a possible selective pressure for the maintenance of this association.

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Molecular Characterization and Physical Mapping of Two Classes of 5S rDNA in the Genomes of <i>Gymnotus sylvius</i> and <i>G. inaequilabiatus</i> (Gymnotiformes, Gymnotidae)

Cited by 15 publications

References 49 publications

New Approach Data in Electric Fish (Teleostei: Gymnotus): Sex Chromosome Evolution and Repetitive DNA

New Approach Data in Electric Fish (Teleostei: Gymnotus): Sex Chromosome Evolution and Repetitive DNA

Comparative Chromosome Mapping of U2 snRNA and 5S rRNA Genes in <b><i>Gymnotus</i></b> Species (Gymnotiformes, Gymnotidae): Evolutionary Dynamics and Sex Chromosome Linkage in <b><i>G</i></b>. <b><i>pantanal</i></b>

Cytogenetic Mapping of H1 Histone and Ribosomal RNA Genes in Hybrids between Catfish Species <b><i>Pseudoplatystoma corruscans</i></b> and <b><i>Pseudoplatystoma reticulatum</i></b>

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