Molecular characterization and phylogenetic analysis of Bacillus pumilus causing trunk bulges of RRIM 3001 superclone rubber tree in Malaysia

Mazlan, S.; Jaafar, Noraini Md; Wahab, Aswad; Sulaiman, Zulkefly; Rajandas, Heraa; Zulperi, Dzarifah

doi:10.1007/s10658-021-02208-9

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…We additionally used the gyrB and pycA genes for the identification of Bacillus strains. Partial sequences of gyrB and pycA were amplified using specific primers [ 28 , 29 ]. The pure DNA products were delivered to Sangon Biotech Co., Ltd. (Shanghai, China) for sequencing.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

et al. 2023

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The adoption of intensive farming has exacerbated disease outbreaks in aquaculture, particularly vibriosis caused by Vibrio parahaemolyticus. The use of probiotics to control V. parahaemolyticus is recognized as a good alternative to antibiotics for avoiding the development of antibiotic-resistant bacteria. In this study, two strains of B. HLJ1 and B. C1 with strong inhibitory activity on V. parahaemolyticus were isolated from aquaculture water and identified as Bacillus subtilis and Bacillus pumilus, respectively. Both B. HLJ1 and B. C1 lacked antibiotic resistance and virulence genes, suggesting that they are safe for use in aquaculture. In addition, these two strains can tolerate acid environments, produce spores, secrete extracellular enzymes, and co-aggregate as well as auto-aggregate with V. parahaemolyticus. B. HLJ1 and B. C1 produced the same anti-V. parahaemolyticus substance, which was identified as AI-77-F and belongs to amicoumacins. Both B. C1 and B. HLJ1 showed inhibitory activity against 11 different V. parahaemolyticus and could effectively control the growth of V. parahaemolyticus in simulated aquaculture wastewater when the concentration of B. C1 and B. HLJ1 reached 1 × 107 CFU/mL. This study shows that B. HLJ1 and B. C1 have great potential as aquaculture probiotics.

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Section: Methodsmentioning

confidence: 99%

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

et al. 2023

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“…Rubber trees have been domesticated for approximately one century, and only four breeding generations have been undertaken thus far (Cheng et al ., 2020 ). Despite that, the yield of rubber trees has increased from 496 kg ha −1 in unselected trees to 3000 kg ha −1 in RRIM 3001, a recently released clone (Mazlan et al ., 2021 ; Priyadarshan, 2017 ). Benefiting from clone improvement, Asia now produces approximately 92% of the NR in the world ( http://www.anrpc.org/ ).…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level wild Hevea brasiliensis genome provides new tools for genomic‐assisted breeding and valuable loci to elevate rubber yield

Cheng

Song

et al. 2023

Plant Biotechnology Journal

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Summary The rubber tree (Hevea brasiliensis) is grown in tropical regions and is the major source of natural rubber. Using traditional breeding approaches, the latex yield has increased by sixfold in the last century. However, the underlying genetic basis of rubber yield improvement is largely unknown. Here, we present a high‐quality, chromosome‐level genome sequence of the wild rubber tree, the first report on selection signatures and a genome‐wide association study (GWAS) of its yield traits. Population genomic analysis revealed a moderate population divergence between the Wickham clones and wild accessions. Interestingly, it is suggestive that H. brasiliensis and six relatives of the Hevea genus might belong to the same species. The selective sweep analysis found 361 obvious signatures in the domesticated clones associated with 245 genes. In a 15‐year field trial, GWAS identified 155 marker–trait associations with latex yield, in which 326 candidate genes were found. Notably, six genes related to sugar transport and metabolism, and four genes related to ethylene biosynthesis and signalling are associated with latex yield. The homozygote frequencies of the causal nonsynonymous SNPs have been greatly increased under selection, which may have contributed to the fast latex yield improvement during the short domestication history. Our study provides insights into the genetic basis of the latex yield trait and has implications for genomic‐assisted breeding by offering valuable resources in this new domesticated crop.

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“…The genus Bacillus that was known well as plant growthpromoting bacteria has been reported (Saxena et al, 2020;Zhang et al, 2015); however, some Bacillus species can cause a variety of plant diseases. For example, Bacillus pumilus is a notorious pathogenic bacteria causing diseases to the host plants, including peach (Saleh et al, 1997), mango (Galal et al, 2006), pear (Li et al, 2009), ginger (Peng et al, 2013), ficus lacor (Hakim et al, 2015), pine (Kovaleva et al, 2015), muskmelon (Song et al, 2018), and rubber (Mazlan et al, 2021). Furthermore, B. megaterium (Hosford Jr, 1982) and B. altitudinis (Elbanna et al, 2014;Liu et al, 2021) were also reported as plant pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Identification of Bacillus species causing bacterial leaf spot of peach in China

Jin

Wang

Zhou

et al. 2022

Journal of Phytopathology

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Peach (Prunus persica L.) is an economically important fruit and is cultivated widely in the world. Bacterial leaf spot (BLS) is a major disease limiting the yield of peach in most peach-producing areas worldwide. To identify the pathogen causing bacterial leaf spot, evaluate its pathogenicity, and explore its host range, the diseased leaves were collected. Phylogenetic analysis of the partial 16S rRNA genes showed that the five

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Molecular characterization and phylogenetic analysis of Bacillus pumilus causing trunk bulges of RRIM 3001 superclone rubber tree in Malaysia

Cited by 4 publications

References 19 publications

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

Chromosome‐level wild Hevea brasiliensis genome provides new tools for genomic‐assisted breeding and valuable loci to elevate rubber yield

Identification of Bacillus species causing bacterial leaf spot of peach in China

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