Molecular characterization and phylogenetic analysis of Babesia and Theileria spp. in ticks from domestic and wild hosts in Sardinia

Chisu, Valentina; Alberti, Alberto; Zobba, Rosanna; Foxi, Cipriano; Masala, Giovanna

doi:10.1016/j.actatropica.2019.05.013

Cited by 21 publications

(26 citation statements)

References 35 publications

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“…(Uilenberg, 2006). High temperature and anemia are the common clinical signs of babesiosis (Chisu et al, 2019). Hemoglobinuria is a typical sign not commonly observed in other protozoan diseases.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle

Farooq¹

2020

PVJ

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Babesiosis is a key tick born disease caused by the protozoal parasites belonging to the genus Babesia, cosmopolitan in nature and infecting enormous range of large ruminants. Study was intended to determine the presence of local isolates of Babesia species (B. bovis, B. bigemina) their molecular characterization and phylogenetic relationship in cattle (district Narowal) Punjab. Blood samples were collected from 200 suspected cattle, from seven villages of selected district. DNA was extracted by using DNA zole, Gene-all Kit, manual method and subjected to PCR for amplification using specific RLB and Universal Primers. Quantification of protozoan parasite was performed by RT PCR using Solis biodine kit. A total of 36 out of 200 animal samples, were found positive by microscopy. PCR positive samples (17) were amplified and bands of strength 520-bp and 800-bp with specific RLB and for universal primers respectively were obtained for Babesia species. These 17 samples were sent for sequencing and by using Clustal W; Bioedit software with Neighbor-joining method phylogenetic tree was established. Current study reported the presence of B. bigemina i.e. 18% (conventional method) in large ruminants of district Narowal, Pakistan, and confirmation by (molecular characterization) PCR (8.5%) and phylogenetic relationship. Phylogenetic results showed that our study has homology with Babesia bigemina strain.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle

Farooq¹

2020

PVJ

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“…The PCR primers and cycling conditions used were the same as those documented in previous studies. 12–14 …”

Section: Methodsmentioning

confidence: 99%

“…The PCR primers and cycling conditions used were the same as those documented in previous studies. [12][13][14] The reactions were performed in automated DNA thermal cyclers (GeneAmp PCR System 9700; Applied Biosystems, Courtaboeuf, France). PCR products were analysed by electrophoresis on 1.5 per cent agarose gels stained with SYBR Safe DNA Gel Stain (Thermo Fisher) and examined under UV transillumination.…”

Section: Detection and Characterisation Of Tickborne Pathogensmentioning

confidence: 99%

Detection of potentially pathogenic bacteria from Ixodes ricinus carried by pets in Tuscany, Italy

Chisu

Foxi

Masu

et al. 2020

Veterinary Record Open

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BackgroundTicks are vectors of disease-causing pathogens that pose a serious threat to animals and people. Dogs and cats are exposed to tick infestation in multiple ways and can easily transport infected ticks into domestic environments and potentially transfer them to people. Pet owners are at increased risk of picking up ticks from their pets and developing tickborne diseases. This study aims to detect the presence of pathogens of potential public health interest in ticks removed from cats and dogs in Tuscany, Italy.MethodsThe collected ticks were screened for the presence of protozoan (Theileria species and Babesia species) and bacterial (Rickettsia species, Anaplasma species, Ehrlichia species, Chlamydia species, Bartonella species and Coxiella burnetii) pathogens using PCR.ResultsPCR and sequencing analysis revealed that 3 per cent of the ticks were PCR-positive for the presence of Rickettsia helvetica DNA, 5 per cent of ticks were PCR-positive for Bartonella henselae DNA, and 46 per cent of ticks were PCR-positive for Chlamydia psittaci and Chlamydia abortus DNA. None of the examined ticks was PCR-positive for Theileria species, Babesia species, Anaplasma species, Ehrlichia canis or Coxiella burnetii DNA.ConclusionThe results of this preliminary study highlight the importance of monitoring companion animals as indicators to evaluate the health status of their owners. Preventive measures are necessary to limit the spread of zoonotic pathogens from companion animals to people within the home environment.

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“…Tick DNAs were screened for the presence of Rickettsia spp., Anaplasma spp., Ehrlichia canis, Coxiella burnetii, Bartonella spp., Chlamydia spp., and Babesia/Theileria spp. by using previously established PCR assays (Chisu et al, 2019(Chisu et al, , 2018a(Chisu et al, , 2018b (Chisu et al, 2019(Chisu et al, , 2018a. A negative control using DNA extracted from uninfected ticks and a positive control were included in each test.…”

Section: Detection and Characterization Of Tick Borne Pathogensmentioning

confidence: 99%

Direct detection of tick-borne pathogens in Ixodes ricinus ticks collected from dogs and cats in Tuscany, Italy

Chisu

Foxi

Masu

et al. 2019

Preprint

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Ticks are able to transmit pathogens which represent an health risk for domestic animals and humans. In this study, ticks morphologically identified as Ixodes ricinus were collected from dogs and cats presented at local veterinary practice in Pistoia, Tuscany. Ticks were examined by PCR in order to detect tick-borne pathogens. Out of 37 I. ricinus ticks analyzed, one tick (3%) was 100% similar to R. helvetica after PCR analyses and sequencing. DNA of Eh. canis was detected in four I. ricinus ticks (12%) . Bartonella spp. DNAs with 100% identity with Bartonella henselae were identified in two I. ricinus (5%) collected from one dog and one cat. One tick showed 100% similarity with C. abortus , while 16 I. ricinus ticks exhibithed DNA of C. psittaci . DNAs of piroplasmids, Anaplasma spp., and C. burnetii were not detected in this study. Findings expand knowledge about the repertoire of tick-borne bacteria present in ticks from Tuscany and indicate that cats and dogs are exposed to tick-borne pathogen which represent a medical risk for their owners.

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Molecular characterization and phylogenetic analysis of Babesia and Theileria spp. in ticks from domestic and wild hosts in Sardinia

Cited by 21 publications

References 35 publications

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle

Molecular Characterization and Phylogenetic Analysis of Babesia Species Isolated from Domestic Cattle

Detection of potentially pathogenic bacteria from Ixodes ricinus carried by pets in Tuscany, Italy

Direct detection of tick-borne pathogens in Ixodes ricinus ticks collected from dogs and cats in Tuscany, Italy

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